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Parasitism by Cotesia flavipes alters the haemocyte population and phenoloxidase activity of the sugarcane borer, Diatraea saccharalis

Published online by Cambridge University Press:  18 May 2012

A.M.A. Mahmoud ,
E.J. De Luna-Santillana ,
X. Guo  and
Mario A. Rodríguez-Pérez
A.M.A. Mahmoud*
Affiliation:
Zoology Department, Assiut University, 71515 Assiut, Egypt Centro de Biotecnología Genómica (CBG), Instituto Politécnico Nacional (IPN), Blvd. del Maestro esq. Elías Piña, Col. Narciso Mendoza, Reynosa, Tamaulipas 88710, México
E.J. De Luna-Santillana
Affiliation:
Centro de Biotecnología Genómica (CBG), Instituto Politécnico Nacional (IPN), Blvd. del Maestro esq. Elías Piña, Col. Narciso Mendoza, Reynosa, Tamaulipas 88710, México
X. Guo
Affiliation:
Centro de Biotecnología Genómica (CBG), Instituto Politécnico Nacional (IPN), Blvd. del Maestro esq. Elías Piña, Col. Narciso Mendoza, Reynosa, Tamaulipas 88710, México
Mario A. Rodríguez-Pérez
Affiliation:
Centro de Biotecnología Genómica (CBG), Instituto Politécnico Nacional (IPN), Blvd. del Maestro esq. Elías Piña, Col. Narciso Mendoza, Reynosa, Tamaulipas 88710, México
*
1Corresponding author (e-mail: [email protected]).
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Abstract

Parasitism of Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) larvae by Cotesia flavipes Cameron (Hymenoptera: Braconidae) or injection of C. flavipes polydnavirus causes numerous alterations in host physiology including developmental arrest, abrogation of host immunity, and biochemical changes in proteins, carbohydrates, glycogen, and lipids. This study focused on changes in haemocyte composition occurring in the cellular immune system of parasitised D. saccharalis larvae. We also analysed the effects of parasitisation by C. flavipes on humoral immunity in terms of phenoloxidase (PO) activity in the host. In nonparasitised D. saccharalis larvae, granular cells represented the main haemocyte type (39%) and plasmatocytes were also present at around 35% among the total haemocytes. The percentages of these two major haemocytes decreased significantly in parasitised larvae after 6 days of parasitoid oviposition and the total haemocyte counts exhibited a significant reduction in parasitised larvae 3 and 6 days following parasitisation. The parasitised larvae also showed a significant decrease in humoral immune capacity as evidenced by reduction of PO activity. Moreover, the plasma had more PO activity than the haemocytes and the parasitised larvae showed less PO activity than the control larvae. This research demonstrates that the parasitism of C. flavipes adversely affects the total haemocyte populations and PO activity of D. saccharalis larvae, which would contribute to host immunosuppression.

Résumé

Le parasitisme des larves de Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) par Cotesia flavipes Cameron (Hymenoptera: Braconidae) ou une injection du virus polyADN C. flavipes cause de nombreuses modifications de la physiologie de l'hôte, y compris un arrêt du développement, la suppression de l'immunité de l'hôte, ainsi que des changements biochimiques dans les protéines, les hydrates de carbone, le glycogène et les lipides. Notre étude examine les changements dans la composition des hémocytes qui se produisent dans le système immunitaire cellulaire des larves parasitées de D. saccharalis. Nous analysons aussi les effets du parasitisme par C. flavipes sur l'immunité humorale reflétée par l'activité de la phénoloxydase (PO) de l'hôte. Chez les larves non parasitées de D. saccharalis, les cellules granulaires représentent le type principal (39%) d'hémocytes et les plasmocytes font environ 35% de l'ensemble des hémocytes. Les pourcentages de ces deux principaux hémocytes diminuent significativement chez les larves parasitées 6 jours après la ponte du parasitoïde et les nombres totaux d'hémocytes connaissent une réduction significative chez les larves parasitées 3 et 6 jours après le début du parasitisme. Les larves parasitées montrent aussi une réduction significative de leur capacité immunitaire humorale comme l'indique la diminution de l'activité de la phénoloxydase. De plus, le plasma a une activité de la PO plus importante que les hémocytes et les larves parasitées ont une activité de la PO inférieure à celle des larves témoins. Notre recherche démontre que le parasitisme par C. flavipes affecte négativement la population totale d'hémocytes et l'activité de la PO chez les larves de D. saccharalis, ce qui contribue à l'immunosuppression chez l'hôte.

Type
Original Article
Information
The Canadian Entomologist , Volume 144 , Issue 4 , 03 August 2012 , pp. 599 - 608
Copyright
Copyright © Entomological Society of Canada 2012

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