Hostname: page-component-78c5997874-4rdpn Total loading time: 0 Render date: 2024-11-08T02:09:55.721Z Has data issue: false hasContentIssue false
  • English
  • Français

A METHOD FOR SEPARATING AND SURFACE STERILIZING THE EGGS OF THE SPRUCE BUDWORM, CHORISTONEURA FUMIFERANA (LEPIDOPTERA: TORTRICIDAE)1

Published online by Cambridge University Press:  31 May 2012

Arthur Retnakaran  and
John French
Arthur Retnakaran
Affiliation:
Insect Pathology Research Institute, Canadian Forestry Service, Sault Ste. Marie, Ontario
John French
Affiliation:
Insect Pathology Research Institute, Canadian Forestry Service, Sault Ste. Marie, Ontario
Get access Rights & Permissions [Opens in a new window]

Abstract

A simple method for separating and surface sterilizing spruce budworm eggs is described. The egg masses are incubated with constant agitation in a 1% solution of NaOH for 10 minutes at 35 °C. The separated eggs are rinsed serially in distilled water, 1% bovine albumen solution, and 70% ethanol, and placed on a moist, sterile filter paper in a petri dish. When surface sterilized eggs are not required the 70% ethanol wash can be omitted.

Type
Articles
Information
The Canadian Entomologist , Volume 103 , Issue 5 , May 1971 , pp. 712 - 716
Copyright
Copyright © Entomological Society of Canada 1971

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Beck, S. D. and Stauffer, J. F.. 1950. An aseptic method for rearing European corn borer larvae. J. econ. Ent. 43: 4–6.CrossRefGoogle Scholar
Bobbitt, J. M. 1963. Thin-layer chromatography. Reinhold, New York. 208 pp.Google Scholar
Condrashoff, S. F. 1967. An extraction method for rapid counts of insect eggs and small organisms. Can. Ent. 49: 300–303.Google Scholar
Grisdale, D. 1970. An improved laboratory method for rearing large numbers of spruce budworm, Choristoneura fumiferana (Lepidoptera: Tortricidae). Can. Ent. 102: 1111–1117.CrossRefGoogle Scholar
McMorran, A. 1965. A synthetic diet for the spruce budworm, Choristoneura fumiferana (Clem.) (Lepidoptera: Tortricidae). Can. Ent. 97: 58–62.Google Scholar
Retnakaran, A. 1970 a. Preliminary results of radiation induced sterility of the male spruce budworm. Bi-mon. Res. Notes, Can. Dep. For. 26: 13–14.Google Scholar
Retnakaran, A. 1970 b. Metepa as a chemosterilant for the adult male spruce budworm, Choristoneura fumiferana. J. econ. Ent. 63: 1394–1395.CrossRefGoogle ScholarPubMed
Retnakaran, A. 1970 c. Blocking of embryonic development in the spruce budworm, Choristoneura fumiferana (Lepidoptera: Tortricidae), by some compounds with juvenile hormone activity. Can. Ent. 102: 1592–1596.CrossRefGoogle Scholar
Retnakaran, A. and Grisdale, D.. 1970. The ovicidal effect of hydrochlorination reaction mixure on the spruce budworm, Choristoneura fumiferana (Lepidoptera: Tortricidae). Ann. ent. Soc. Am. 63: 907–909.CrossRefGoogle Scholar