Hostname: page-component-78c5997874-v9fdk Total loading time: 0 Render date: 2024-11-06T01:02:24.945Z Has data issue: false hasContentIssue false
  • English
  • Français

INDUCTION OF PHEROMONE PRODUCTION IN FEMALES OF HELIOTHIS VIRESCENS (F.) AND H. SUBFLEXA (GN.) (LEPIDOPTERA: NOCTUIDAE) DURING THE PHOTOPHASE

Published online by Cambridge University Press:  31 May 2012

P.E.A. Teal ,
A. Oostendorp  and
J.H. Tumlinson
P.E.A. Teal
Affiliation:
Insect Attractants, Behavior and Basic Biology Research Laboratory, USDA, ARS, PO Box 14565, Gainesville, Florida, USA 32604
A. Oostendorp
Affiliation:
Insect Attractants, Behavior and Basic Biology Research Laboratory, USDA, ARS, PO Box 14565, Gainesville, Florida, USA 32604
J.H. Tumlinson
Affiliation:
Insect Attractants, Behavior and Basic Biology Research Laboratory, USDA, ARS, PO Box 14565, Gainesville, Florida, USA 32604
Get access Rights & Permissions [Opens in a new window]

Abstract

Production of sex pheromone was induced during the photophase, a time during which pheromone is not normally produced, in females of Heliothis virescens (F.) and H. subflexa (Gn.) by injection of homogenates and partially purified extracts of the brain – subesophageal ganglion complex of conspecific females or H. zea (Boddie) females or synthetic pheromone biosynthesis activating neuropeptide (PBAN). The amount of (Z)-11-hexadecenal, the major component of the pheromone of both species, increased during the first 60 min after injection of PBAN then leveled off and declined in H. virescens. In H. subflexa, the amount of this aldehyde increased during the first 120 min and declined after 240 min. Studies indicated that extracts of the brain – subesophageal ganglion complex that did not contain neurally produced biogenic amines induced production of as much pheromone as did homogenates containing these compounds. Dose-response studies indicated that the optimal dose of synthetic PBAN for induction of pheromone during a 60-min incubation was 5.0 pmol. In vivo maintenance of pheromone production required continuous stimulation of the pheromone gland.

Résumé

Une injection d’homogénats et d’extraits partiellement purifiés du complexe cerveau – ganglion suboesophagien de femelles de même espèce ou de femelles de Heliothis zea ou encore une injection du neuropeptide synthétique activateur de la synthèse de phéromone (PBAN) provoque la production de la phéromone sexuelle chez les femelles de Heliothis virescens (F.) et de H. subflexa (Gn.) durant la photophase, une période où la phéromone n’est pas normalement produite. Chez H. virescens, la quantité de (Z)-11-hexadéccnal, la constituant principal de la phéromone chez les deux espèces, augmente durant les 60 premières minutes après l’injection de PBAN pour ensuite se stabiliser et décliner. Chez H. subftexa, la teneur de cet aldéhyde augmente pendant les 120 premières minutes et commence à décroître après 240 min, Les extracts de cerveau – ganglion suboesophagien qui ne contenaient pas d’aminés biogéniques produites par le système nerveux ont provoqué autant de production de phéromone que ceux qui en contenaient. Des dosages indiquent que la dose optimale de PBAN synthétique pour la production de phéromone lors d’une incubation de 60 min est de 5,0 pmol. Le maintien de la production de phéromone in vivo exige une stimulation continuelle de la glande à phéromone.

[Traduit par la rédaction]

Type
Articles
Information
The Canadian Entomologist , Volume 125 , Issue 2 , April 1993 , pp. 355 - 366
Copyright
Copyright © Entomological Society of Canada 1993

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Altstein, M., Gazit, Y., and Dunkelblum, E.. 1990. Regulation of sex pheromone biosynthesis in Chrysodeixis chalcites and Heliothis peltigera by PBAN. pp. 303–307 in Borkovec, A.B., and Masler, E.P. (Eds.), Insect Neurochemistry and Neurophysiology. Humana Press, Clifton, NJ.Google Scholar
Altstein, M., Harel, M., and Dunkelblum, E.. 1989. Effect of a neuroendocrine factor on sex pheromone biosynthesis in the tomato looper, Chrysodexis chalcites (Lepidoptera: Noctuidae). Insect Biochemistry 19: 645–649.CrossRefGoogle Scholar
Ando, T., Arima, R., Uchiyama, M., Hiromichi, N., Inoue, T., and Suzuki, A.. 1988. Pheromone biosynthesis activating neuropeptide hormone in heads of the silkworm moth. Agricultural and Biological Chemistry 52: 881–883.Google Scholar
Bestmann, H.J., Herrig, M., Attygalle, A.B., and Hupe, M.. 1989. Regulatory steps in sex pheromone biosynthesis in Mamestra brassicae (Lepidoptera: Noctuidae). Experientia 45: 778–781.CrossRefGoogle Scholar
Chauthani, A.R., and Callahan, P.S.. 1967. The nervous system of the corn earworm moth, Heliothis zea (Lepidoptera: Noctuidae). Annals of the Entomological Society of America 60: 248–255.CrossRefGoogle Scholar
Christensen, T.C., Itagaki, H., Teal, P.E.A., Jasensky, R.D., Tumlinson, J.H., and Hildebrand, J.G.. 1991. Innervation and neural regulation of the sex pheromone gland in female Heliothis moths. Proceedings of the National Academy of Sciences 88: 4971–4975.CrossRefGoogle ScholarPubMed
Christensen, T.C., Lehman, H.K., Teal, P.E.A., Itagaki, H., Tumlinson, J.H., and Hildebrand, J.G.. 1992. Diel changes in the presence and physiological actions of octopamine in the female sex-pheromone glands of heliothine moths. Insect Biochemistry and Molecular Biology 22: 841–849.CrossRefGoogle Scholar
Cusson, M., and McNeil, J.. 1989. Involvement of juvenile hormone in the regulation of sex pheromone release activities in a moth. Science 243: 210–212.CrossRefGoogle Scholar
Gazit, J., Dunkelblum, E., Benichio, M., and Altstein, M.. 1990. Effect of synthetic PBAN and derived peptides on sex pheromone biosynthesis in Heliothis peltigera (Lepidoptera: Noctuidae). Insect Biochemistry 20: 853–858.CrossRefGoogle Scholar
Goldstein, D.S. 1983. Modified sample preparation for high performance liquid chromatographic-electrochemical assay of urinary catecolamines. Journal of Chromatography 175: 174–177.CrossRefGoogle Scholar
Heath, R.R., McLaughlin, J.R., Proshold, F., and Teal, P.E.A.. 1991. Periodicity of female sex pheromone titer and release in Heliothis subflexa and H. virescens (Lepidoptera: Noctuidae). Annals of the Entomological Society of America 84: 182–189.CrossRefGoogle Scholar
Jaffe, H., Raina, A.K., and Hayes, D.K.. 1986. HPLC isolation and purification of pheromone biosynthesis activating neuropeptide of Heliothis zea. pp. 219–224in Borkovec, A.B., and Gelman, D.B. (Eds.), Insect Neurochemistry and Neurophysiology. Humana Press, Clifton, NJ.Google Scholar
Jurenka, R.A., Fabrais, G., and Roelofs, W.L.. 1991. Hormonal control of female sex pheromone biosynthesis in the redbanded leafroller moth, Argyrotaenia velutinana. Insect Biochemistry 21: 81–89.CrossRefGoogle Scholar
Jurenka, R.A., Jacquin, E., and Roelofs, W.L.. 1991 a. Control of the pheromone biosynthetic pathway in Helicoverpa zea by pheromone biosynthesis activating neuropeptide. Archives of Insect Biochemistry and Physiology 17: 81–91.CrossRefGoogle Scholar
Jurenka, R.A., Jacquin, E., and Roelofs, W.L.. 1991 b. Stimulation of pheromone biosynthesis in the moth Helicoverpa zea: Action of a brain hormone on pheromone glands involves Ca2+ and cAMP as second messengers. Proceedings of the National Academy of Sciences 88: 8621–8625.CrossRefGoogle Scholar
Kingan, T.C., Raina, A.K., Blackburn, M., and Ma, M.. 1990. Distribution of PBAN-like immunoreactivity in the CNS of the corn earworm, Heliothis zea. Society of Neurosciences Abstract 16: 856.Google Scholar
Kitamura, A., Hagasawa, H., Kataoka, H., Inoue, T., Matsumoto, S., Ando, T., and Suzuki, A.. 1989. Amino acid sequence of pheromone-biosynthesis-activating neuropeptide (PBAN) of the silkworm, Bombyx mori. Biochemical and Biophysical Research Communications 163: 520–526.CrossRefGoogle ScholarPubMed
Martinez, T., and Camps, F.. 1988. Stimulation of sex pheromone production by head extracts in Spodoptera littoralis at different times of the photoperiod. Archives of Insect Biochemistry and Physiology 9: 211–220.CrossRefGoogle Scholar
Ohguchi, Y., Tatsuki, S., Usui, K., Arai, K., Kurhara, M., Uchiumi, K., and Fukama, J.. 1985. Hormone-like substance present in cephalic organs of the female, Chilo suppressalis (Walker) (Lepidoptera: Pyralidae) and controlling sex pheromone production. Japanese Journal of Applied Entomology and Zoology 29: 265–269.CrossRefGoogle Scholar
Rafaeli, A., Hirsch, J., Soroker, V., Kamensky, B., and Raina, A.K.. 1991. Spatial and temporal distribution of pheromone biosynthesis activating neuropeptide in Helicoverpa (Heliothis) armigera using RIA and in vitro bioassay. Archives of Insect Biochemistry and Physiology 18: 119–129.CrossRefGoogle ScholarPubMed
Rafaeli, A., and Soroker, V.. 1989. Influence of diel rhythm and brain hormone on pheromone production in two Lepidopteran species. Journal of Chemical Ecology 15: 447–455.CrossRefGoogle ScholarPubMed
Rafaeli, A., Soroker, V., Kamensky, B., and Raina, A.K.. 1990. Action of pheromone activating neuropeptide on in vitro pheromone glands of Helicoverpa armigera females. Journal of Insect Physiology 36: 641–646.CrossRefGoogle Scholar
Raina, A.K., Jaffe, H., Kempe, T.G., P. Keim, Blocker, R.W., Falco, H.M., Riley, C.T., Klun, J.A., Ridgway, R.L., and Hayes, D.K.. 1989. Identification of a neuropeptide hormone that regulates sex pheromone production in female moths. Science 244: 796–798.CrossRefGoogle ScholarPubMed
Raina, A.K., and Klun, J.. 1984. Brain factor control of sex pheromone production in female corn earworm moth. Science 225: 531–533.CrossRefGoogle ScholarPubMed
Tang, J.D., Charlton, R.E., Carde, R.T., and Yin, C.J.. 1987. Effect of allatectomy and ventral nerve cord transection on calling, pheromone emission and pheromone production in Lymantria dispar. Journal of Insect Physiology 33: 469–476.CrossRefGoogle Scholar
Tang, J.D., Charlton, R.E., Jurenka, R.A., Wolf, W.A., Phelan, P.L., Streng, L., and Roelofs, W.L.. 1989. Regulation of pheromone biosynthesis by a brain hormone in two moth species. Proceedings of the National Academy of Sciences 86: 1806–1810.CrossRefGoogle ScholarPubMed
Teal, P.E.A., Carslyle, T.C., and Tumlinson, J.H.. 1985. Epidermal glands in terminal abdominal segments of female Heliothis virescens (F.) (Lepidoptera: Noctuidae). Annals of the Entomological Society of America 76: 242–247.CrossRefGoogle Scholar
Teal, P.E.A., and Tumlinson, J.H.. 1989. Neurohormonal induction of pheromone biosynthesis by Heliothis zea (Boddie) during the photophase. The Canadian Entomologist 121: 43–46.CrossRefGoogle Scholar
Teal, P.E.A., and Tumlinson, J.H.. 1991. Sex pheromone production in moths: Endogenous regulation of initiation and inhibition. pp. 78–86 in Hedin, P.A. (Ed.), Naturally Occurring Pest Bioregulators. American Chemical Society, Washington, DC.Google Scholar
Teal, P.E.A., Tumlinson, J.H., and Oberlander, H.. 1989. Neural regulation of sex pheromone biosynthesis in Heliothis moths. Proceedings of the National Academy of Sciences 86: 2488–2492.CrossRefGoogle ScholarPubMed