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EVIDENCE OF HONEYDEW FEEDING IN BLACK FLIES (DEPTERA: SIMULIIDAE)

Published online by Cambridge University Press:  31 May 2012

Steven G. Burgin  and
Fiona F. Hunter
Steven G. Burgin
Affiliation:
Department of Biological Sciences, Brock University, St. Catharines, Ontario, Canada L2S 3A1
Fiona F. Hunter*
Affiliation:
Department of Biological Sciences, Brock University, St. Catharines, Ontario, Canada L2S 3A1
*
1Author to whom all correspondence should he addressed.
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Abstract

Black flies (Diptera: Simuliidae) were collected from a tamarack stand, Larix laricina (Du Roi) Koch, heavily infested with Adelges lariciatus (Patch) (Homoptera: Adelgidae). Insect nets were used to sweep the tamarack branches to capture black flies associated with the trees. Six black fly species were sweep-netted, with 85.5% of all flies belonging to Simulium venustum Say complex. Samples of honeydew and the crops and midguts of individual black flies were tested by thin layer chromatography using fructose, glucose, sucrose, turanose, melezitose, raffinose, and stachyose as standards. The sugars fructose, glucose, sucrose, raffinose, and stachyose were found in the adelgid honeydew samples. Of the 201 black flies tested, 194 contained sugars, which occurred in 16 combinations. It is argued that stachyose can be used to indicate when black flies have fed on the adelgid honeydew. We conclude that 49.7% of the S. venustum collected from the tamarack had fed recently on this honeydew source. In addition, it was observed that black flies reared in the laboratory readily ingested freshly excreted and older (dry) honeydew when presented with branches from the tamarack stand.

Résumé

Des mouches noires (Diptera : Simuliidae) ont été récoltées dans une forêt de mélèzes [Larix laricina (Du Roi) Koch] fortement infestée de pucerons Adelges lariciatus (Patch) (Homoptera : Adelgidae). Des filets fauchoirs ont été utilisés pour capturer les mouches noires dans les rameaux des mélèzes. Six espèces ont été capturées, mais 85,5% des mouches noires appartenaient au complexe Simulium venustum Say. Des échantillons de miellat et des prélèvements de jabots et d’estomacs de mouches noires ont été soumis à la technique de chromatographie en couche mince et les sucres fructose, glucose, sucrose, turanose, mélézitose, raffinose et stachyose ont servi à la calibration. Le fructose, le glucose, le sucrose, le raffinose et le stachyose ont été trouvés dans les échantillons de miellat des pucerons. Des 201 mouches noires expérimentales, 194 contenaient des sucres présents en 16 combinaisons différentes. Le stachyose s’est avéré être le sucre tout indiqué pour déterminer si les mouches noires s’étaient nourries de miellat. Au total, 49,7% des S. venustum capturées dans la forêt de mélèzes s’étaient nourries de miellat depuis peu. De plus, des mouches noires de laboratoire mises en présence de rameaux provenant de la forêt de mélèzes se sont nourries de miellat frais et de miellat plus ancien (séché) présents sur les branches.

[Traduit par la Rédaction]

Type
Articles
Information
The Canadian Entomologist , Volume 129 , Issue 5 , October 1997 , pp. 859 - 869
Copyright
Copyright © Entomological Society of Canada 1997

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References

Auclair, J.L. 1963. Aphid feeding and nutrition. Annual Review of Entomology 8: 1439–490.CrossRefGoogle Scholar
Bacon, J.S.D., and Dickinson, B.. 1955. The origin of the trisaccharide melezitose. Biochemistry Journal 61: XV.Google Scholar
Bailey, R.W. 1962. Colour reactions between sugars and diphenylamine – urea and diphenylamine – p – anisidine on paper chromatograms. Journal of Chromatography 8: 57–62.CrossRefGoogle Scholar
Brenner, R.J., and Cupp, E.W.. 1980. Preliminary observations on parity and nectar feeding in the black fly, Simulium jenningsi. Mosquito News 40(3): 390–393.Google Scholar
Burgin, S.G., and Hunter, F.F.. 1997. Sugar meal sources of female black flies (Diptera: Simuliidae): a four-habitat study. Canadian Journal of Zoology 75: 1066–1072.CrossRefGoogle Scholar
Byrne, D.N., and Miller, W.B.. 1990. Carbohydrate and amino acid composition of phloem sap and honeydew produced by Bemisia tabaci. Journal of Insect Physiology 36(6): 433–439.CrossRefGoogle Scholar
Crosskey, R.W. 1990. The natural history of black flies. John Wiley and Sons, Chichester, U.K.711 pp.Google Scholar
Damonte, A., Lombard, A., Tourn, M.L., and Cassone, M.C.. 1971. A modified solvent system and multiple detection technique for the separation and identification of mono- and oligosaccharides on cellulose thin layers. Journal of Chromatography 60: 203–211.CrossRefGoogle Scholar
Davies, D.M. 1953. Longevity of black flies in captivity. Canadian Journal of Zoology 31: 304–312.Google Scholar
Davies, D.M., and Peterson, B.V.. 1956. Observations on the mating, feeding, ovarian development, and oviposition of adult black flies (Simuliidae, Diptera). Canadian Journal of Zoology 34: 615–655.CrossRefGoogle Scholar
Davies, D.M., Peterson, B.V., and Wood, D.M.. 1962. The black flies (Diptera: Simuliidae) of Ontario. Part I. Adult identification and distribution with descriptions of six new species. Proceedings of the Entomological Society of Ontario 92: 70–154.Google Scholar
Davis, D.W., McDougall, E.M., Hendrix, D.L., Steele, T.L., Adaskaveg, J.E., and Butler, E.E.. 1993. Air particulates associated with the ash whitefly. Journal of the Air & Waste Management Association 43: 1116–1121.Google Scholar
Downes, J.A. 1974. The feeding habits of adult Chironomidae. Entomologisk Tidskrift 95: 84–90.Google Scholar
Hendrix, D.L., Wei, Y., and Leggett, J.E.. 1992. Homopteran honeydew sugar composition is determined by both the insect and plant species. Comparative Biochemistry and Physiology B Comparative Biochemistry 101(1–2): 23–27.CrossRefGoogle Scholar
Hocking, B. 1953. The intrinsic range and speed of flight of insects. Transactions of the Royal Entomological Society of London 104: 223–345.Google Scholar
Hocking, B., and Pickering, L.R.. 1954. Observations on the bionomics of some northern species of Simuliidae (Diptera). Canadian Journal of Zoology 32: 99–119.CrossRefGoogle Scholar
Hudson, C.S. 1946. Melezitose and turanose. Advances in Carbohydrate Chemistry 2: 1–36.Google Scholar
Hudson, C.S., and Sherwood, S.F.. 1920. The occurrence of melezitose in honey. Journal of the American Chemical Society 42: 116–125.CrossRefGoogle Scholar
Hunter, D.M. 1977. Sugar-feeding in some Queensland black flies (Diptera: Simuliidae). Journal of Medical Entomology 14(2): 229–232.Google Scholar
Hunter, F.F. 1990. Ecological, morphological and behavioural correlates to cytospecies in the Simulium venustum/verecundum complex (Diptera: Simuliidae). Ph.D. thesis, Queen's University, Kingston, Ont.Google Scholar
Hunter, F.F., Burgin, S.G., and Wood, D.M.. 1994. New techniques for rearing black flies from pupae (Diptera: Simuliidae). Journal of the American Mosquito Control Association 10(3): 456–459.Google Scholar
Hussain, A., Forrest, J.M.S., and Dixon, A.F.G.. 1974. Sugar, organic acid, phenolic acid and plant growth regulator content of extracts of honeydew of the aphid Myzus persicae and of its host plant, Raphanus sativus. Annals of Applied Biology 78: 65–73CrossRefGoogle ScholarPubMed
Lewis, D.J., and Domoney, C.R.. 1966. Sugar meals in Phlebotominae and Simuliidae (Diptera). Proceedings of the Royal Entomological Society of London Series A General Entomology 41: 175–179.Google Scholar
MacVicker, J.A.K., Moore, J.S., Molyneux, D.H., and Maroli, M.. 1990. Honeydew sugars in wild-caught Italian phlebotomine sandflies (Diptera: Psychodidae) as detected by high performance liquid chromatography. Bulletin of Entomological Research 80: 339–344.CrossRefGoogle Scholar
McCreadie, J.W., Colbo, M.H., and Hunter, F.F.. 1994. Notes on sugar-feeding and wild mammalian hosts of Newfoundland black flies (Diptera: Simuliidae). Journal of Medical Entomology 31(4): 566–570.Google Scholar
Mittler, T.E. 1958. Studies on the feeding and nutrition of Tuberolachinus salignus (Gmelin) (Homoptera, Aphididae): II. The nitrogen and sugar composition of ingested phloem sap and excreted honeydew. Journal of Experimental Biology 35: 74–84.Google Scholar
Moore, J.S., Kelly, T.B., Killick-Kendrick, R., Killick-Kendrick, M., Wallbanks, K.R., and Molyneux, D.H.. 1987. Honeydew sugars in wild-caught Phlebotomus ariasi detected by high performance liquid chromatography (HPLC) and gas chromatography (GC). Medical and Veterinary Entomology 1: 427–434.CrossRefGoogle ScholarPubMed
Muller, H. 1873. The fertilization of flowers. Translated by D'Arcy W. Thompson. London. 1883. 669 pp.Google Scholar
Robertson, C. 1928. Flowers and Insects. Carlinville, Ill. 221 pp.Google Scholar
Schutz, S.J., and Gaugler, R.. 1989. Honeydew-feeding behavior of salt marsh horse flies (Diptera: Tabanidae). Journal of Medical Entomology 26(5): 471–473.CrossRefGoogle Scholar
Smart, J. 1943. Simulium feeding on ivy flowers. Entomologist 76: 20–21.Google Scholar
Wallbanks, K.R., Moore, J.S., Bennett, L.R., Soren, R., Molyneux, D.H., Carlin, J.M., and Perez, J.E.. 1990. Aphid derived sugars in the neotropical sandfly–Lutzomyia peruensis. Tropical Medicine and Parasitology 42: 60–62.Google Scholar
Walsh, J.F., and Garms, R. 1980. The detection of plant sugars in Simulium damnosum s.l. by means of the cold anthrone test. Transactions of the Royal Society of Tropical Medicine and Hygiene 74: 811–813.Google Scholar
Watanabe, M. 1977. Observation on nectar-sucking behavior and parous rates of five species of blackflies. Japanese Journal of Sanitary Zoology 31: 215–222. [In Japanese with English summary.]Google Scholar
Wenk, P. 1965. On the biology of bloodsucking simuliids (Diptera). I. Insemination rate of females visiting flowers and attacking blood hosts. Zeitschrift fur Morphologie und Okologie der Tiere 55: 656–670.CrossRefGoogle Scholar