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Establishing abiotic and biotic factors necessary for reliable male pheromone production and attraction to pheromones by female plum curculios Conotrachelus nenuphar (Coleoptera: Curculionidae)

Published online by Cambridge University Press:  31 March 2014

Virginia Hock ,
Gérald Chouinard ,
Éric Lucas ,
Daniel Cormier ,
Tracy Leskey ,
Starker Wright ,
Aijun Zhang  and
André Pichette
Virginia Hock*
Affiliation:
Laboratoire de Production Fruitière Intégrée, Institut de recherche et de développement en agroenvironnement (IRDA), 335, chemin des Vingt-Cinq Est, Saint-Bruno-de-Montarville, Québec, Canada J3V 0G7 Laboratoire de lutte biologique, Département des sciences biologiques, Université du Québec à Montréal (UQAM), Case postale 8888, succursale Centre-ville, Montréal, Québec, Canada H3C 3P
Gérald Chouinard
Affiliation:
Laboratoire de Production Fruitière Intégrée, Institut de recherche et de développement en agroenvironnement (IRDA), 335, chemin des Vingt-Cinq Est, Saint-Bruno-de-Montarville, Québec, Canada J3V 0G7
Éric Lucas
Affiliation:
Laboratoire de lutte biologique, Département des sciences biologiques, Université du Québec à Montréal (UQAM), Case postale 8888, succursale Centre-ville, Montréal, Québec, Canada H3C 3P
Daniel Cormier
Affiliation:
Laboratoire de Production Fruitière Intégrée, Institut de recherche et de développement en agroenvironnement (IRDA), 335, chemin des Vingt-Cinq Est, Saint-Bruno-de-Montarville, Québec, Canada J3V 0G7
Tracy Leskey
Affiliation:
United States Department of Agriculture, Agricultural Research Service – Appalachian Fruit Research Station, 2217 Wiltshire Road, Kearneysville, West Virginia 25430, United States of America
Starker Wright
Affiliation:
United States Department of Agriculture, Agricultural Research Service – Appalachian Fruit Research Station, 2217 Wiltshire Road, Kearneysville, West Virginia 25430, United States of America
Aijun Zhang
Affiliation:
Invasive Insect Biocontrol and Behaviour Laboratory, United States Department of Agriculture (USDA), Agricultural Research Service – Plant Science Institute, 10300 Baltimore Avenue, Beltsville, Maryland 20705-2350, United States of America
André Pichette
Affiliation:
Université du Québec à Chicoutimi, 555, boul. de l'Université, Chicoutimi, Québec, Canada G7H 2B1
*
1Corresponding author: (e-mail: [email protected]).
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Abstract

The plum curculio (PC), Conotrachelus nenuphar Herbst (Coleoptera: Curculionidae), is a key pest of stone and pome fruit in North America. Though grandisoic acid (GA) was identified as a male-produced aggregation pheromone for this species, other components likely exist, as have been identified for various curculionids. To better determine these components, an understanding of the conditions necessary for optimum pheromone production and attraction is needed, this is essential for the improvement of monitoring techniques and to achieve better biological control. The goal of this study was to determine the biotic and abiotic factors influencing both the response to pheromones and pheromone production. Tests were conducted in a dual-choice still-air vertical olfactometer using live male PCs as odour sources and live females as responders, to determine which physiological factors (age, number of males, mating status) influenced female response to males. Head-space collections of GA production under various conditions (airflow rate and frequency, collection zone strata, variation of humidity, temperature, and presence of a harbourage) were also done, as were electroantennograms (EAG) using synthetic pheromone mixtures. Results revealed that for both strains, the odour of two virgin mature males elicited significantly greater and more consistent attraction from mature virgin females than other ages and numbers of males when compared with the control. Head-space collections indicate that male PC have increased production of GA under high humidity in the presence of fruit, indicating that these conditions are necessary for optimal pheromone production and collection. EAG studies revealed significant responses to GrandLures I, II, III/IV and to the positive enantiomer of GA, and the amplitude of the signal varied with concentration. Our data identify the optimal physiological state and conditions at which pheromone collections should be performed, and what physiological life stages respond to these stimuli. These results have implications for optimising monitoring tools for this serious crop pest. This species has a northern univoltine strain and a southern multivoltine strain, both of which were examined in this study.

Type
Behaviour & Ecology
Information
The Canadian Entomologist , Volume 146 , Issue 5 , October 2014 , pp. 528 - 547
Copyright
© Entomological Society of Canada 2014 

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Footnotes

Subject editor: Véronique Martel

References

Akotsen-Mensah, C. 2010. Ecology and management of plum curculio, Conotrachelus nenuphar (Coleoptera: Curculionidae) in Alabama peaches [online]. Ph.D. dissertation. Auburn University, Alabama, United States of America. Available from http://etd.auburn.edu/etd/bitstream/handle/10415/2300/PhD%20Dissertation%208-3-2010.pdf?sequence=2 [accessed 15 January 2014].Google Scholar
Alm, S.R. and Hall, F.R. 1986. Antennal sensory structures of Conotrachelus nenuphar (Coleoptera: Curculionidae). Annals of the Entomological Society of America, 79: 324–333.CrossRefGoogle Scholar
Altuzar, A., Malo, E.A., Gonzalez-Hernandez, H., and Rojas, J.C. 2007. Electrophysiological and behavioural responses of Scyphophorus acupunctatus (Col., Curculionidae) to Agave tequilaza volatiles . Journal of Applied Entomolology, 13: 121–127.Google Scholar
Amis, A.A. and Snow, J.W. 1985. Conotrachelus nenuphar handbook of insect rearing. Elsevier Science Publishers, Amsterdam, The Netherlands. Pp. 227–235.Google Scholar
Armstrong, J.S. 2011. Boll weevil (Coleoptera: Curculionidae) response to and volatilization rates of GrandLure when combined with varying doses of Eugenol in the extended-life pheromone lure. Journal of Economic Entomology, 103: 356–359.Google Scholar
Aspi, J. and Hoffman, AA. 1998. Female encounter rates and fighting costs of males are associated with lek size in Drosophila mycetophaga. Behavioural Ecology and Sociobiology, 42: 163–169.Google Scholar
Bobb, M.L. 1952. The life history and control of the plum curculio in Virginia. Bulletin of the West Virginia University Agricultural Experiment Station, 453: 1–30.Google Scholar
Burkholder, W.E. 1990. Practical use of pheromones and other attractants for stored-product insect. In Behavior-modifying chemicals for insect management. Edited by R. Ridgway, R. Silverstein, and M. Inscoe. Marcel Dekker, New York, New York, United States of America. Pp. 531–537.Google Scholar
Butkewich, S.L. and Prokopy, R.J. 1993. The effects of short-range host odour stimuli on host fruit finding and feeding behaviour of plum curculio adults (Coleoptera: Curculionidae). Journal of Chemical Ecology, 19: 825–835.Google Scholar
Butkewich, S.L., Prokopy, R.J., and Green, T.A. 1987. Discrimination of occupied host fruit by plum curculio females (Coleoptera: Curculionidae). Journal of Chemical Ecology, 13: 1833–1841.Google Scholar
Chouinard, G., Hill, S.B., and Vincent, C. 1993. Spring behaviour of the plum curculio, (Coleoptera Curculionidae), within caged dwarf apple trees in spring. Journal of Insect Behaviour, 5: 385–394.Google Scholar
Chouinard, G., Hill, S.B., and Vincent, C. 1994. Spatial distribution and movements of plum curculio adults within caged apple trees. Entomologica Experimentalis et Applicata, 70: 129–142.Google Scholar
Coffelt, J.A. and Burkholder, W.E. 1972. Reproductive biology of the cigarette beetle Lasioderma serricorne. 1. Quantitative laboratory bioassay of the female sex pheromone from females of different ages. Annals of the Entomological Society of America, 65: 447–450.CrossRefGoogle Scholar
Cross, J.V., Helen Hesketh, H., Jay, C.N., Hall, D.R., Innocenzi, P.J., Farman, D.I., et al. 2006. Exploiting the aggregation pheromone of strawberry blossom weevil Anthonomus rubi Herbst (Coleoptera: Curculionidae): part 1. Development of lure and trap. Crop Protection, 25: 144–154.Google Scholar
De Graaf, J., Govender, P., Schoeman, A.S., and Viljoen, A. 2005. Efficacy of pseudostem and pheromone seasonal trapping of the banana weevil, Cosmopolites sordidus in South Africa. International Journal of Pest Management, 51: 209–218.Google Scholar
Dickens, J.C 1986. Orientation of boll weevil, Anthonomus grandis Boh. (Coleoptera: Curculionidae) to pheromone and volatile host compound in the laboratory. Journal of Chemical Ecology, 12: 91–98.Google Scholar
Dickerson, W.A., Ridgeway, R.L., and Planer, F.R. 1987. Southeastern boll weevil eradication program, improved pheromone trap and program status. Proceedings of Beltwide Cotton Research and Production Conferences. Dallas, Texas. 10–11 January 1967. National Cotton Council of America, Memphis, Tennessee, United States of America. Pp. 335–337.Google Scholar
Dixon, B.M., Prokopy, R.J., and Schultz, B.B. 1999. Influence of weather and time of day on plum curculio (Coleoptera: Curculionidae) tree canopy entry behaviors and evaluation of traps for predicting fruit injury. Journal of Entomological Science, 34: 191–202.CrossRefGoogle Scholar
Eller, F.J. and Bartelt, R.J. 1996. Grandisoic acid a male produced aggregation pheromone from the plum curculio, Conotrachelus nenuphar . Journal of Natural Products, 59: 451–453.CrossRefGoogle Scholar
Eller, F.J., Bartelt, R.J., Shasha, B.S., Schuster, D.J., Riley, D.G., Stansly, P.A., et al. 1994. Aggregation pheromone for the pepper weevil, Anthonomus eugenii Cano (Coleoptera: Curculionidae): identification and field activity. Journal of Chemical Ecology, 20: 1537–1555.Google Scholar
Garman, P. and Zappe, M.P. 1929. Control studies on the plum curculio in Connecticut apple orchards. Connecticut Agricultural Station, Bulletin, 301: 373–437.Google Scholar
Hallett, R.H., Oehlschlager, A.C., and Borden, J.B. 1999. Pheromone trapping protocols for the Asian palm weevil, Rhynchophorus ferrugineus (Coleoptera: Curculionidae). International Journal of Pest Management, 45: 231–237.CrossRefGoogle Scholar
Hardee, D.D. 1982. Mass trapping and trap cropping of the boll weevil, Anthonomus grandis Boheman. In Insect suppression with controlled release pheromone systems. Volume 2. Edited by A.F. Kydonieus and M. Berosa. CRC Press, Boca Raton, Florida, United States of America. Pp. 65–71.Google Scholar
Hardee, D.D., McKibben, G.H., Rummel, D.R., Huddleston, P.M., and Coppedge, J.R. 1974. Response of boll weevils to component ratios and doses of the pheromone, GrandLure. Environmental Entomology, 3: 135–138.CrossRefGoogle Scholar
Hedin, P.A., Dollar, D.A., Collins, J.K., Dubois, J.G., Mulder, P.G., Hedger, G.H., et al. 1997. Identification of male pecan weevil pheromone. Journal of Chemical Ecology, 23: 965–977.Google Scholar
Hock, V., Chouinard, G., Lucas, É., and Cormier, D. 2013. Mites affect plum curculio (Coleoptera: Curculionidae) behavioural responses to attractive volatiles. The Canadian Entomologist, 145: 82–87.Google Scholar
Hoffman, E.J., Combs, A.B., and Whalon, M.E. 2004. Reproductive development of northern and southern strains of plum curculio (Coleoptera: Curculionidae). Journal of Economic Entomology, 97: 27–32.CrossRefGoogle Scholar
Hoffman, E.J., Vanderjagt, J., and Whalon, M.E. 2007. Pyriproxyfen reproduction in prediapause northern strain plum curculio (Conotrachelus nenuphar Herbst). Pest Management Science, 63: 835–840.Google Scholar
Hölgund, J. and Alatalo, R. 1995. Leks. Princeton University, Princeton, New Jersey, United States of America.Google Scholar
Hoyt, S.C., Leeper, J.R., Brown, G.C., and Croft, B.A. 1983. Basic biology and management components for insect IPM. In Integrated management of insect pests of pome and stone fruits. Edited by B.A. Croft and S.C. Hoyt. Wiley, New York, New York, United States of America. Pp. 93–151.Google Scholar
Innocenzi, P.J., Hall, D.R., and Cross, J.V. 2001. Components of male aggregation pheromone of strawberry blossom weevil, Anthonomus rubi Herbst (Coleoptera: Curculionidae). Journal of Chemical Ecology, 27: 1203–1218.Google Scholar
Jaffé, K., Sánchez, P., Cerda, H., Hernández, J.V., Jaffé, R., Urdaneta, N., et al. 1993. Chemical ecology of Rhynchophorus palmarum: attraction to host plants and to a male-produced aggregation pheromone. Journal of Chemical Ecology, 19: 1703–1720.Google Scholar
Johnson, A.W. and Hays, S.B. 1969. Laboratory mating behaviour of the plum curculio. Journal of Economic Entomology, 62: 438–440.CrossRefGoogle ScholarPubMed
Jones, T.M., Balmford, A., and Quinnell, R.J. 2000. Adaptive female choice for middle-aged mates in a lekking sand fly. Proceedings of the Royal Society of London Biological Sciences, 267: 681–686.Google Scholar
Jutsum, A.R. and Gordon, R.F.S. 1989. Pheromones: importance to insects and role in pest management. In Insect pheromones in plant protection. Edited by A.R. Jutsum and R.F.S. Gordon. John Wiley and Sons, Chichester, United Kingdom. Pp. 1–13.Google Scholar
Keitt, T., Lewis, M., and Holt, R. 2001. Allee effects, invasion pinning and species’ borders. American Naturalist, 157: 203–216.Google Scholar
Klassen, W., Ridgway, R.L., and Inscoe, M. 1982. Chemical attractants in integrated pest management programs. In Insect suppression with controlled release pheromone systems. Volume 1. Edited by A.F. Kydonieus and M. Berosa. CRC Press, Boca Raton, Florida, United States of America. Pp. 65–71.Google Scholar
Kuwahara, Y., Fukami, H., Ishii, S., Matsumura, F., and Burkholder, W.E. 1975. Studies on the isolation and bioassay of the sex pheromone of the drugstore beetle, Stegobium paniceum (Coleoptera: Anobiidae). Journal of Chemical Ecology, 1: 413–422.CrossRefGoogle Scholar
Landolt, P.J. and Phillips, T.W. 1997. Host plant influences on sex pheromone behavior of phytophagous insects. Annual Review of Entomology, 42: 371–391.CrossRefGoogle ScholarPubMed
Le Blanc, J.P.R. 1992. Trapping and monitoring techniques for plum curculio, Conotrachelus nenuphar (Herbst), (Coleoptera: Curculionidae) in a southwestern Quebec apple orchard. Ph.D. dissertation. McGill University, St.-Anne-de-Bellevue, Québec, Canada.Google Scholar
Le Blanc, J.P.R., Hill, S.B., and Paradis, R.O. 1984. Oviposition in scout-apples by plum curculio, Contrachelus nenuphar (Herbst) (Coleoptera: Curculionidae) and its relationship to subsequent damage. Environmental Entomology, 13: 286–291.Google Scholar
Lee, J.C., Negróón, J.F., McElwey, S.J., Williams, L., Witcosky, J.J., Popp, J.B., et al. 2011. Biology of the invasive banded elm bark beetle (Coleoptera: Scolytidae) in the western United States. Annals of the Entomological Society of America, 104: 705–717.Google Scholar
Leskey, T.C., Hancock, T.J., and Wright, S.E. 2010. Host tree-related differences in trap captures and electroantennogram activity for the plum curculio, Conotrachelus nenuphar (Herbst) (Coleoptera: Curculionidae). The Canadian Entomologist, 142: 284–293.Google Scholar
Leskey, T.C. and Prokopy, R.J. 2001. Adult plum curculio (Coleoptera: Curculionidae) attraction to fruit and conspecific odours. Annals of the Entomological Society of America, 94: 275–288.CrossRefGoogle Scholar
Leskey, T.C. and Prokopy, R.J. 2002. Developing a branchmimicking trap for adult plum curculios. Entomolologica Experimentalis et Applicata, 102: 253–259.Google Scholar
Leskey, T.C., Prokopy, R.J., Wright, S.E., Phelan, P.L., and Haynes, L.W. 2001. Evaluation of individual components of plum odor as potential attractants for adult plum curculios. Journal of Chemical Ecology, 27: 1–17.Google Scholar
Leskey, T.C., Wright, S.E., Anger, W., Chouinard, G., Cormier, D., Pichette, A., et al. 2009. Electroantennogram technique for Conotrachelus nenuphar (Coleoptera: Curculionidae). Environmental Entomology, 38: 870–878.Google Scholar
Leskey, T.C., Zhang, A., and Herzog, M. 2005. Nonfruiting host tree volatile blends: novel attractants for the plum curculio, Conotrachelus nenuphar (Coleoptera: Curculionidae). Environmental Entomology, 34: 785–793.Google Scholar
Moller, A.P. and Legendre, S. 2001. Allee effect, sexual selection and demographic stochasticity. Oikos, 92: 27–34.CrossRefGoogle Scholar
Nielsen, B.S. and Jensen, T.S. 1993. Spring dispersal of Sitona lineatus: the use of aggregation pheromone trap for monitoring. Entomologica Experimentalis et Applicata, 66: 21–30.CrossRefGoogle Scholar
Ocellachain, D.P. and Pyan, N.F. 1977. Production and perception of pheromones by the beetle Tribolium confusum . Journal of Insect Physiology, 23: 1303–1309.Google Scholar
Otte, D. 1974. Effects and functions in the evolution of signaling systems. Annual Review of Ecology and Systematics, 5: 385–417.Google Scholar
Padula, A.L. and Smith, E.H. 1971. Reproductive incompatibility between univoltine males and multivoltine females of the plum curculio. Annals of the Entomological Society of America, 64: 665–668.Google Scholar
Phillips, J.K. and Burkholder, W.E. 1981. Evidence for a male-produced aggregation pheromone in the rice weevil. Journal of Economic Entomology, 74: 539–542.Google Scholar
Piñero, J.C., Agnello, A.M., Tuttle, A., Leskey, T.C., Faubert, H., Koehler, G., et al. 2011. Effectiveness of odor-baited trap trees for plum curculio (Coleoptera: Curculionidae) monitoring in commercial apple orchards in the Northeast. Journal of Economic Entomology, 104: 1613–1621.Google Scholar
Piñero, J.C. and Prokopy, R.J. 2003. Field evaluation of plant odor and pheromonal combinations for attracting plum curculios. Journal of Chemical Ecology, 29: 2735–2748.Google Scholar
Piñero, J.C. and Prokopy, R.J. 2004. Predicting plum curculio immigration into apple orchards in Massachusetts: degrees days versus tree phenology. Fruit Notes, 69: 1–7.Google Scholar
Piñero, J.C., Wright, S.E., and Prokopy, R.J 2001. Response of plum curculio (Coleoptera: Curculionidae) to odor-baited traps near woods. Journal of Economic Entomology, 94: 1386–1397.Google Scholar
Prokopy, R.J., Jacome, I., Gray, E., Trujillo, G., Ricci, M., and Piñero, J.C. 2004. Using odor-baited trap trees as sentinels to monitor plum curculio (Coleoptera: Curculionidae) in apple orchards. Journal of Economic Entomology, 97: 511–517.Google Scholar
Prokopy, R.J., Cooley, S.S., and Phelan, P.L. 1995. Bioessay approaches to assessing behavioural responses of plum curculio adults (Coleoptera: Curculionidae) to host fruit odour. Journal of Chemical Ecology, 21: 1073–1084.Google Scholar
Prokopy, R.J., Wirth, C.B., and Leskey, T.C. 1999. Movement of plum curculio adults toward host trees and traps: fight versus walking. Enomologica Experimantalis et Applicata, 91: 385–392.Google Scholar
Prokopy, R.J. and Wright, S.E. 1998. Plum curculio (Coleoptera: Curculionidae) responses to unbaited pyramid and cone traps. Journal of Economic Entomology, 91: 226–234.Google Scholar
Quaintance, A.L. and Jenne, E.L. 1912. The plum curculio. United States Department of Agriculture, Bureau of Entomology Bulletin, 103: 13–218.Google Scholar
Racette, G., Chouinard, G., Hill, S.B., and Vincent, C. 1991. Activity of adult plum curculio (Coleoptera: Curculionidae) on apple trees in spring. Journal of Economic Entomology, 84: 1827–1832.Google Scholar
Racette, G., Chouinard, G., Vincent, C., and Hill, S.B. 1992. Ecology and management of plum curculio, Conotrachelus nenuphar (Coleoptera: Curculionidae), in apple orchards. Phytoprotection, 73: 85–100.Google Scholar
Ridway, R.L. and Inscoe, M.N. 1990. Role of the boll weevil pheromone in pest management. In Behavior-modifying chemicals for insect management. Edited by R. Ridgway, R. Silverstein, and M. Inscoe. Marcel Dekker Inc., New York, New York, United States of America. Pp. 437–471.Google Scholar
Rochat, D., Malosse, C., Lettere, M., Ducrot, P.H., Zagatti, P., Renou, M., et al. 1991. Male-produced aggregation pheromone of the American palm weevil, Rhynchophorus palmarum (L.) (Coleoptera: Curculionidae): collection, identification, electrophysiological activity and laboratory bioassay. Journal of Chemical Ecology, 17: 2127–2141.Google Scholar
Sarai, D.S. 1969. Seasonal history of the plum curculio in the Missouri Ozarks. Journal of Economic Entomology, 62: 1222–1224.Google Scholar
Shelly, T.E. and Whittier, T.S. 1997. Lek behaviour of insects. In The evolution of mating systems in insects and arachnids. Edited by B. Crespi and J.C. Choe. Cambridge Press, Cambridge, United Kingdom. Pp. 273–293.Google Scholar
Siegel, S. and Castellan, N.J. 1988. Nonparametric statistics for the behavioural sciences, 2nd edition. McGraw–Hill, New York, New York, United States of America.Google Scholar
Silverstein, R.M. 1990. Practical use of pheromones and other behavior-modifying compounds:overview. In Behavior-modifying chemicals for insect management. Edited by R. Ridgway, R. Silverstein, and M. Inscoe. Marcel Dekker Inc., New York, New York, United States of America. Pp. 1–8.Google Scholar
Smart, L.E., Blight, M.M., Pickett, J.A., and Pye, B.J. 1994. Development of field strategies incorporating semiochemicals for the control of the pea and bean weevil, Sitona lineatus L. Crop Protection, 13: 127–135.Google Scholar
Smith, E.H. 1957. A method for rearing plum curculio under laboratory conditions including some biological observations. Journal of Economic Entomology, 50: 187–190.Google Scholar
Smith, E.H. and Flessel, J.K. 1968. Hibernation of the plum curculio and its spring migration to host trees. Journal of Economic Entomology, 61: 193–203.Google Scholar
Smith, E.H. and Salkeld, E.H. 1964. Ovary development and oviposition rates in the plum curculio, Conotrachelus nenuphar (Coleoptera: Curculionidae). Annals of the Entomological Society of America, 57: 781–787.CrossRefGoogle Scholar
Spurgeon, D.W. 2003. Age dependence of pheromone production by the boll weevil (Coleoptera: Curculionidae). Environmental Entomology, 32: 31–38.Google Scholar
Szendrei, Z., Averill, A., Alborn, H., and Rodriguez-Saona, C. 2011. Identification and field evaluation of attractants for the cranberry weevil, Anthonomus musculus Say. Journal of Chemical Ecology, 37: 387–397.Google Scholar
Thompson, J.R. 1932. Sex differentiation of adults of Conotrachelus nenuphar . Journal of Economic Entomology, 25: 807–810.Google Scholar
Tinzaara, W., Gold, C.S., Dicke, M., Huis, A.V., and Ragama, P.E. 2007. Host plant odours enhance the responses of adult banana weevil to the syntheticaggregation pheromone cosmoluret® . International Journal of Pest Management, 53: 127–137.Google Scholar
Tumlinson, J.H., Hardee, D.D., Gueldner, R.C., Thompson, A.C., Hedin, P.A., and Minyard, J.P. 1969. Sex pheromones produced by male boll weevils: isolation, identification and synthesis. Science, 166: 1010–1012.Google Scholar
Tumlinson, J.H., Klein, M.G., Doolittle, R.E., Ladd, T.L., and Proveaux, A.T. 1977. Identification of the female Japanese beetle sex pheromone: inhibition of male response by an enantiomer. Science, 197: 789–792.Google Scholar
Vincent, C. and Roy, M. 1992. Entomological limits to biological control programs in Québec apple orchards. Acta Phytopathologica Academiae Scientiarum Hungaricae, 27: 649–657.Google Scholar
Weissling, T.J. and Giblin-Davis, R.M. 1993. Water loss dynamics and humidity preference of Rhynchophorus cruentatus (Coleoptera: Curculionidae) adults. Environmental Entomology, 22: 94–98.Google Scholar
Weissling, T.J., Giblin-Davis, R.M., Center, B.J., and Hiyakawa, T. 1994. Flight behaviour and seasonal trapping of Rhynchophorus cruentatus (Coleoptera: Curculionidae). Annals of the Entomological Society of America, 87: 641–647.Google Scholar
Weldon, C.W. 2007. Influence of male aggregation size on female visitation in Bactrocera tryoni (Froggatt) (Diptera: Tephritidae). Australian Journal of Entomology, 46: 29–34.Google Scholar
Wigglesworth, V.B. 1953. The principles of insect physiology. Methuen and Co. Ltd., London, United Kingdom.Google Scholar
Yang, H., Yang, W., Liang, X.-Y, Yang, M.-F., Yang, C.-P., Zhu, T.-H., et al. 2011. The EAG and behavioural responses of Batocera horsfieldi (Coleoptera: Cerambycidae) to the composition of volatiles. Journal of the Kansas Entomological Society, 84: 217–231.Google Scholar
Zhang, X., Luckhart, S., Tu, Z., and Pfeiffer, D.G. 2010. Analysis of Wolbachia strains associated with Conotrachelus nenuphar (Coleoptera: Curculionidae) in the eastern United States. Environmental Entomology, 39: 396–405.Google Scholar
Zhang, X. and Pfeiffer, D.G. 2008. Evaluation of reproductive compatibility of interstrain matings among plum curculio populations in the eastern United States. Environmental Entomology, 37: 1208–1213.Google Scholar
Zhang, X., Tu, Z., Luckhart, S., and Pfeiffer, D.G. 2008. Genetic diversity of plum curculio (Coleoptera: Curculionidae) among geographical populations in the eastern United States. Annals of the Entomological Society of America, 101: 824–832.Google Scholar
Zhuge, P.-P., Luo, S.-L., Wang, M.-Q., and Zhang, G. 2010. Electrophysiological responses of Batocera horsfieldi (Hope) adults to plant volatiles. Jounral of Applied Entomology, 134: 600–607.Google Scholar