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ENZYME POLYMORPHISM IN THE CORN EARWORM, HELIOTHIS ZEA (LEPIDOPTERA: NOCTUIDAE). HEMOLYMPH ESTERASE POLYMORPHISM

Published online by Cambridge University Press:  31 May 2012

Douglas K. Sell ,
Gregory S. Whitt ,
Robert L. Metcalf  and
Lu-ping Kan Lee
Douglas K. Sell
Affiliation:
Illinois Natural History Survey and Illinois Agricultural Experiment Station, Urbana
Gregory S. Whitt
Affiliation:
Illinois Natural History Survey and Illinois Agricultural Experiment Station, Urbana
Robert L. Metcalf
Affiliation:
Illinois Natural History Survey and Illinois Agricultural Experiment Station, Urbana
Lu-ping Kan Lee
Affiliation:
Illinois Natural History Survey and Illinois Agricultural Experiment Station, Urbana
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Abstract

Esterases present in the hemolymph of last instar Heliothis zea (Boddie) were separated by polyacrylamide gel electrophoresis. Four systems, all polymorphic, were observed. One system, Est-II, appeared especially suited for the study of some of the important population processes of this species; these include host–plant relations, migration, and the effects of environmental pressures such as pesticides on the species.

Est-II was classified as a carboxylesterase, being sensitive only to organophosphate inhibitors. Dichlorvos at 10−5M completely inhibited Est-II activity.

Populations collected from corn from Tifton, Ga., and in two locations in Illinois, at approximately the same time were very similar genetically when compared on the basis of Est-II allele frequencies. A population collected from leguminous hosts in Tifton, Ga., at the same time as the above three populations was different genetically.

Est-IIc, an allele normally rare or absent in the populations studied, was present at a high frequency in one Urbana population collection. Est-IIc may be useful in studying the long-range dispersal of H. zea.

Type
Articles
Information
The Canadian Entomologist , Volume 106 , Issue 7 , July 1974 , pp. 701 - 709
Copyright
Copyright © Entomological Society of Canada 1974

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References

REFERENCES

Balakrishnan, V. and Sanghvi, L. D.. 1968. Distance between populations on the basis of attribute data. Biometrics 24: 859–865.CrossRefGoogle Scholar
Booth, G. M. and Whitt, G. S.. 1970. Histochemical specificity of cholinesterases to phenylthioacetate in differentiated neural tissues of insects and teleosts. Tissue & Cell 2: 521–528.CrossRefGoogle ScholarPubMed
Bush, F. M., Price, J. R., and Townsend, J. I.. 1970. Plasma esterases, their definitions and status as isozymes in the house sparrow. Int. J. Biochem. 1: 85–107.CrossRefGoogle Scholar
Bush, G. L. and Huettel, R. N.. 1972. Starch gel electrophoresis of tephritid proteins: A manual of techniques. Population Genetics Project, Phase I. In International biological program working group on fruit flies. 56 pp.Google Scholar
Dessauer, H. C. and Nevo, E.. 1969. Geographic variation of blood and liver proteins in cricket frogs. Biochem. Genet. 3: 171–188.CrossRefGoogle ScholarPubMed
Hardwick, D. F. 1965. The corn earworm complex. Mem. ent. Soc. Can., No. 78. 124 pp.Google Scholar
Knipling, E. F. 1970. Suppression of pest lepidoptera by releasing partially sterile males. Bioscience 20: 465–470.CrossRefGoogle Scholar
Kojima, K., Gillespie, J., and Tobari, Y. N.. 1970. A profile of Drosophila species' enzymes assayed by electrophoresis. I. Number of alleles, heterozygosities, and linkage disequilibrium in glucose-metabolizing systems and some other enzymes. Biochem. Genet. 4: 627–637.CrossRefGoogle ScholarPubMed
Levene, H. 1949. On a matching problem arising in genetics. Ann. math. Statist. 20: 91–94.CrossRefGoogle Scholar
Markert, C. L. and Møller, F.. 1959. Multiple forms of enzymes: Tissue, ontogenetic and species specific patterns. Proc. U.S. natn. Acad. Sci. 45: 753–763.CrossRefGoogle ScholarPubMed
Quaintance, A. L. and Brues, C. T.. 1905. The cotton bollworm. Bull. Bur. Ent. U.S. Dep. Agric., No. 50. 155 pp.Google Scholar
Wallace, B. 1968. Topics in population genetics. Norton, New York.Google Scholar
Whitten, M. J. 1970. Genetics of pests in their management, pp. 119–135. In. Rabb, R. L. and Guthrie, F. E. (Eds.), Concepts of pest management. North Carolina State University, Raleigh.Google Scholar