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DIGESTIVE ENZYMES OF SIXTH-INSTAR LARVAE OF THE PALE WESTERN CUTWORM, AGROTIS ORTHOGONIA (LEPIDOPTERA: NOCTUIDAE)

Published online by Cambridge University Press:  31 May 2012

M. R. Khan  and
R. Kasting
M. R. Khan
Affiliation:
Research Station, Canada Department of Agriculture, Lethbridge, Alberta
R. Kasting
Affiliation:
Research Station, Canada Department of Agriculture, Lethbridge, Alberta
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Abstract

Results of a qualitative survey of some digestive enzymes of sixth-instar larvae of the pale western cutworm are reported. An extract prepared from the mesenteron (midgut) and its contents showed activity for the enzymes, proteinase, aminopeptidase, dipeptidase, amylase, α-glucosidasc, α-galactosidase, and lipase. There was also evidence for a β-glucosidase activity in the brei prepared from the mesenteron. A brei of salivary glands showed activities for α-glucosidase and amylase, as well as a strong proteinase. These enzymes were not found in brei prepared from the stomodaeum (foregut) or proctodaeum (hindgut). There was no evidence for polygalacturonase, celiulase, β-galactosidase, or carboxypeptidase in any part of the digestive system.Values of pH for extracts from the different parts of the digestive system were: proctodaeum, 7.2; mesenteron, 8.2; stomodaeum, 7.8; and salivary glands, 7.8.

Type
Articles
Information
The Canadian Entomologist , Volume 101 , Issue 5 , May 1969 , pp. 494 - 499
Copyright
Copyright © Entomological Society of Canada 1969

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References

Činčerová, A. 1961. Über den Einfluss der Humussaure auf die Veranderungen der freien Zucker in Weizenpflanzen. Symp. Humus and Plant, Praha, Brno28.1X–6 X.Google Scholar
Colowick, S. P., and Kaplan, N. O.. 1955. Methods in enzymology, I. Academic Press, Inc., New York.Google Scholar
Hocking, B., and Depner, K. R.. 1961. Larval nutrition in Agrotis orthogonia (Lepidoptera: Phalaenidae): Digestive enzymes. Ann. ent. Soc. Am. 54: 86–89.CrossRefGoogle Scholar
Jermyn, M. A., and Tompkins, R. G.. 1950. The chromatographic examination of the products of the action of pectinase on pectin. Biochem. J. 47: 437–442.CrossRefGoogle ScholarPubMed
Kasting, R., and McGinnis, A. J.. 1961. Hollow-stemmed spring wheats during growth. II. Food values determined with the pale western cutworm, Agrotis orthogonia Morr. (Lepidoptera: Noctuidae). Can. J. Zool. 39: 273–280.CrossRefGoogle Scholar
Kasting, R., and McGinnis, A. J.. 1962. Nutrition of the pale western cutworm, Agrotis orthogonia (Lepidoptera: Noctuidae). IV. Amino acid requirements determined with glucose-U-C14. J. Insect Physiol. 8: 97–103.CrossRefGoogle Scholar
Kasting, R., and McGinnis, A. J.. 1963. Resistance of plants to insects — the role of insect nutrition. Agric. Inst. Rev. 18: 9–11.Google Scholar
Kasting, R., and McGinnis, A. J.. 1967. An artificial diet and some growth factor requirements for the pale western cutworm. Can. J. Zool. 43: 787–796.CrossRefGoogle Scholar
Khan, M. R. 1961. Feeding and digestion of Dysdercus fasciatus (Sign.). Ph.D. Thesis, University College of North Wales, Bangor.Google Scholar
Khan, M. R., and Ford, J. B.. 1967. The distribution and localization of digestive enzymes in the alimentary canal and salivary glands of the cotton stainer, Dysdercus fasciatus. J. Insect Physiol. 13: 1619–1628.CrossRefGoogle Scholar
Lopatecki, L. E., Longair, E. L., and Kasting, R.. 1962. Quantitative changes of soluble carbohydrates in stems of solid- and hollow-stemmed wheats during growth. Can. J. Botany 40: 1123–1128.CrossRefGoogle Scholar
McGinnis, A. J., and Kasting, R.. 1962. Nutrition of the pale western cutworm, Agrotis orthogonia Morr. (Lepidoptera: Noctuidae). V. Utilization of protein labelled with carbon-14. J. Nutr. 76: 333–340.CrossRefGoogle Scholar
McGinnis, A. J., and Kasting, R.. 1969. Digestibility studies with cellulose-U-C14 for the pale western cutworm, Agrotis orthogonia Morr. J. Insect Physiol. 15: 5–10.CrossRefGoogle Scholar
Miller, D. F. 1958. Composition of cereal grains and forages. Comm. on Feed Comp., Agric. Board, Nat. Acad. Sci. Nut. Res. Council Publ. 585.Google Scholar
Pickard, B. G., and Thimann, K. V.. 1966. Geotropic response of wheat coleoptiles in absence of amyloplast starch. J. Gen. Physiol. 49: 1065–1086.CrossRefGoogle ScholarPubMed
Pickford, G. E., and Dorris, F.. 1934. Micro-methods for the detection of proteases and amylases. Science 80: 317–319.CrossRefGoogle ScholarPubMed
Roberts, D. W. A. 1950. Qualitative and quantitative determination of wheat leaf carbohydrates. Can. J. Res. C 28: 754–779.CrossRefGoogle Scholar
Soo Hoo, C. F., and Dudziński, Anne. 1967. Digestion by the larva of the pruinose scarab, Sericesthis geminata. Ent. exa. appl. 10: 7–15.CrossRefGoogle Scholar
Swingle, M. S. 1928. Digestive enzymes of the Oriental Fruit Moth. Ann. ent. Soc. Am. 21: 469–475.CrossRefGoogle Scholar