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Analysis of genetic diversity in an invasive population of Asian long-horned beetles in Ontario, Canada

Published online by Cambridge University Press:  02 April 2012

Maureen E. Carter ,
Michael T. Smith ,
Jean J. Turgeon  and
Richard G. Harrison
Maureen E. Carter*
Affiliation:
Department of Entomology, Comstock Hall, Cornell University, Ithaca, New York 14853-2601, United States of America
Michael T. Smith
Affiliation:
United States Department of Agriculture, Agricultural Research Service, Beneficial Insect Introduction Research Unit, Newark, Delaware 19713, United States of America
Jean J. Turgeon
Affiliation:
Great Lakes Forestry Centre, Canadian Forest Service, Natural Resources Canada, Sault Ste. Marie, Ontario, Canada P6A 2E5
Richard G. Harrison
Affiliation:
Department of Ecology and Evolutionary Biology, Cornell University, Ithaca, New York 14853, United States of America
*
1Corresponding author (e-mail: [email protected]).
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Abstract

Adult Asian long-horned beetles, Anoplophora glabripennis (Motschulsky) (Coleoptera: Cerambycidae: Lamiinae), were discovered in Ontario, Canada, in 2003 in the vicinity of a commercial warehouse. Trees were heavily scarred with signs of attack and larvae and adult beetles were common, suggesting that there had been multiple generations at the site. We amplified 16 microsatellite loci from 326 beetles to examine genetic diversity in this population. Based on Hardy – Weinberg equilibrium, 6 of 16 loci were monomorphic and 8 were not, indicating nonrandom mating. Measures of microsatellite genetic diversity and mitochondrial DNA haplotype diversity were significantly lower than those in A. glabripennis from China and Korea but were not significantly different from those in the New York City population. The proportion of different multilocus genotypes in the Ontario population was lower than in the populations in New York City and Linden, New Jersey. These results suggest that limited genetic diversity in the Ontario population has not hampered reproduction of this invasive insect. This genetic signature is common in other invasive species, likely because a population is founded by a few closely related individuals, or a large founding population suffers subsequent genetic bottlenecks.

Résumé

Des adultes du longicorne asiatique, Anoplophora glabripennis (Motschulsky) (Coleoptera : Cerambycidae : Lamiinae), ont été découverts en Ontario, Canada, en 2003 près d’un entrepôt commercial. Les arbres portaient de fortes lacérations dues à l’attaque des coléoptères et les larves et les adultes y étaient nombreux, ce qui laisse croire qu’il y avait eu plusieurs générations à ce site. Nous avons examiné la diversité génétique de la population en amplifiant 16 locus microsatellites chez 326 coléoptères. Six des 16 locus sont monomorphes et 8 locus ne suivent pas l’équilibre Hardy – Weinberg, ce qui indique que les accouplements ne sont pas aléatoires. Les mesures de diversité génétique des microsatellites et de diversité des haplotypes d’ADN mitochondrial sont significativement plus faibles que celles d’A. glabripennis en Chine et en Corée, mais elles ne diffèrent pas significativement des mesures de la population de la ville de New York. La proportion de génotypes différents à locus multiples est plus faible dans la population d’Ontario que dans les populations de la ville de New York et de Linden (New Jersey). Ces résultats indiquent que la diversité génétique restreinte de la population d’Ontario n’a pas nui à la reproduction de cet insecte envahissant. Une telle signature génétique est commune chez d’autres espèces envahissantes, vraisemblablement parce que la population est fondée sur un petit nombre d’individus fortement apparentés ou alors parce qu’une population fondatrice de grande taille subit plus tard des goulots d’étranglement génétiques.

[Traduit par la Rédaction]

Type
Articles
Information
The Canadian Entomologist , Volume 141 , Issue 6 , December 2009 , pp. 582 - 594
Copyright
Copyright © Entomological Society of Canada 2009

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