Hostname: page-component-78c5997874-j824f Total loading time: 0 Render date: 2024-11-06T06:18:18.203Z Has data issue: false hasContentIssue false
  • English
  • Français

Amino Acid Requirements for the Wheat Stem Sawfly Determined with Glucose-U-C14 after Vacuum-infiltration1

Published online by Cambridge University Press:  31 May 2012

R. Kasting  and
A. J. McGinnis
R. Kasting
Affiliation:
Canada Agriculture Research Station, Lethbridge, Alberta
A. J. McGinnis
Affiliation:
Canada Agriculture Research Station, Lethbridge, Alberta
Get access Rights & Permissions [Opens in a new window]

Abstract

Glucose-U-C14 was incorporated into immature larvae of the wheat stem sawfly, Cephus cinctus Nort., by vacuum-infiltration. These insects were too small to be conveniently injected and could not be easily fed on artificial diets. About half of them survived the infiltration treatment. C14O2 was produced by the organism showing that the radioactive substrate was metabolized. Of the amino acids isolated from the larvae, proline, alanine, glutamic acid, serine, aspartic acid, and glycine contained relatively large quantities of carbon-14 indicating biosynthesis, and are classed as nutritionally non-essential. In contrast, arginine, isoleucine, leucine, lysine, phenylalanine, threonine, tyrosine, and valine contained little, if any, radioactivity and are classed as nutritionally essential. The concentrations of some of the amino acids in the larval tissues are also presented.

Type
Articles
Information
The Canadian Entomologist , Volume 96 , Issue 8 , August 1964 , pp. 1133 - 1137
Copyright
Copyright © Entomological Society of Canada 1964

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Auclair, J. L., and Cartier, J. J.. 1963. Rearing the pea aphid on a chemically defined diet. Science 142: 1068–1069.CrossRefGoogle ScholarPubMed
Friend, W. 1958. Nutritional requirements of phytophagous insects. Annu. Rev. Ent. 3: 57–74.CrossRefGoogle Scholar
Kasting, R., Davis, G. R. F. and McGinnis, A. J.. 1962. Nutritionally essential and non-essential amino acids for the prairie grain wireworm, Ctenicera destructor Brown, determined with glucose-U-C14. J. Ins. Physiol. 8: 589–596.CrossRefGoogle Scholar
Kasting, R., and Delwiche, C. C.. 1958. Ornithine, citrulline, and arginine metabolism in watermelon seedlings. Plant Physiol. 33: 350–354.CrossRefGoogle ScholarPubMed
Kasting, R., and McGinnis, A. J.. 1958. Use of glucose labelled with carbon-14 to determine the amino acids essential for an insect. Nature, Lond. 182: 1380–1381.Google Scholar
Kasting, R., and McGinnis, A. J.. 1960. Use of glutamic acid-U-C14 to determine nutritionally essential amino acids for larvae of the blow fly, Phormia regina. Canad. J. biochem. Physiol. 38: 1229–1234.CrossRefGoogle ScholarPubMed
Kasting, R., and McGinnis, A. J.. 1962. Nutrition of the pale western cutworm, Agrotis orthogonia Morr. (Lepidoptera: Noctuidae). IV. Amino acid requirements determined with glucose-U-C14. J. Ins. Physiol. 8: 97–103.CrossRefGoogle Scholar
Plaisted, P. H. 1958. Clearing amino acid solutions of plant extracts for paper chromatography. Contr. Boyce Thompson Inst. 19: 231–244.Google Scholar
Strong, F. E. 1963. Fatty acids: In vivo synthesis by the green peach aphid, Myzus persicae (Sulzer). Science 140: 983–984.CrossRefGoogle ScholarPubMed
Williams, H. H. et al. , 1954. Estimation of growth requirements for amino acids by assay of the carcass. J. biol. Chem. 208: 277–286.Google Scholar