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The use of serology in evaluating invertebrate prey-predator relationships : a review

Published online by Cambridge University Press:  10 July 2009

P. F. L. Boreham  and
C. E. Ohiagu
P. F. L. Boreham*
Affiliation:
Department of Zoology and Applied Entomology, Imperial College of Science and Technology, Prince Consort Road, London, SW7 2AZ, UK
C. E. Ohiagu
Affiliation:
Department of Zoology and Applied Entomology, Imperial College of Science and Technology, Prince Consort Road, London, SW7 2AZ, UK
*
*Address for correspondence: Imperial College Field Station, Silwood Park, Ascot, Berks.
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Abstract

Several methods, including the use of serology, are available for evaluating prey-predator relationships. Two basic methods involving precipitation and agglutination reactions have been utilised, both of which depend on the production of specific antisera with high titres. A critical account is presented of methods of preparing antisera, with particular reference to the amount of antigen required, the route of inoculation and testing antisera, together with a review of the five precipitation and two agglutination techniques available. The main limitations of serological methods are tje specificity of the arrtisera and the difficulties in quantifying results unless assumptions are made as to the number of prey eaten at each feed. Details of studies employing serological techniques to identify the predators of specific prey and the food od invertebrates are given intwo tables. The value of serological techniques is discussed in relation to the ecology of invertebrates by considering, as examples, te insect fauna on broon (Sarothamnus scoparius), predation of mosquitoes and the food of triclads. Errors in the quantification of predation may occur by including positive reactions resulting from carrion feeding or material ingested acidentally on plants by phytophagous arthropods.

Type
Review Article
Information
Bulletin of Entomological Research , Volume 68 , Issue 2 , July 1978 , pp. 171 - 194
Copyright
Copyright © Cambridge University Press 1978

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References

Anderson, J. M. (1972). Food and feeding of Notiophilus biguttatus F. (Coleoptera, Carabidae).—Revue Ecol. & Biol. Sol 9, 177–184.Google Scholar
Ascoli, M. (1902). Über den mechanismus der Albumeneree durch Eierweiss.—Münch. med. Wschr. 49, 398–401.Google Scholar
Ashey, J. W. (1974). A study of arthropod predation of Pieris rapae L. using serological and exclusion techniques.—J. appl. Ecol. 11, 419–425.CrossRefGoogle Scholar
Aspock, H. & Kunz, C. (1967). Untersuchungen über die ökologie des Tahyna-Virus.— J (Abt. I) 203, 1–24.Google Scholar
Baldwin, W. F., James, H. G. & Welch, H. E. (1955). A study of predators of mosquito larvae and pupae with a radio-active tracer.—Can. Ent. 87, 350–356.CrossRefGoogle Scholar
Becker, M. (1975). The biology and population ecology of Macrosteles sexnotatus (Fallen) (Cicadellidae, Hemiptera).—Ph.D. thesis, Univ. London.Google Scholar
Boddinoton, M. J. & Mettrick, D. F. (1974). The distribution, abundance, feeding habits, and population biology of the immigrant triclad Dugesia polychroa (Platyhelminthes: Turbellaria) in Toronto Harbour, Canada.—J. Anim. Ecol. 43, 681–699.CrossRefGoogle Scholar
Boreham, P. F. L. (1972). Serological identification of arthropod bloodmeals and its application.—PANS 18, 205–209.Google Scholar
Boreham, P. F. L. (1975). Some applications of bloodmeal identifications in relation to the epidemiology of vector-borne tropical diseases.—J. trop. Med. Hyg. 78, 83–91.Google Scholar
Boreham, P. F. L. & Gill, G. S. (1973). Serological identification of reptile feeds of Glossina.—Acta trop. 30, 356–365.Google ScholarPubMed
Brooke, M. M. & Proske, H. O. (1946). Precipitin test for determining natural insect predators of immature mosquitoes.—J. natn. Malar. Soc. 5, 45–56.Google Scholar
Coaker, T. H. & Williams, D. A. (1963). The importance of some Carabidae and Staphylinidae as predators of the cabbage root fly Erioschia brassicae (Bouchè).— Entomologia exp. appl. 6, 156–164.CrossRefGoogle Scholar
Csizmas, L. (1960). Preparation of formalinized erythrocytes.—Proc. Soc. exp. Biol. Med. 103, 157–160.CrossRefGoogle ScholarPubMed
Danthanarayana, W. (1969). Population dynamics of the weevil Sitonia regensteinensis (Hbst.) on broom.—J. Anim. Ecol. 38, 1–18.CrossRefGoogle Scholar
Davies, R. W. (1969). The production of antisera for detecting specific triclad antigens in the gut contents of predators.—Oikos 20, 248–260.CrossRefGoogle Scholar
Davies, R. W. & Reynoldson, T. B. (1971). The incidence and intensity of predation on lake-dwelling triclads in the field.—J. Anim. Ecol. 40, 191—214.CrossRefGoogle Scholar
DeBach, P. (1946). An insecticidal check method for measuring the efficacy of entomophagous insects.—J. econ. Ent. 39, 695–697.CrossRefGoogle ScholarPubMed
DeBach, P. (1955). Validity of the insecticidal check method as a measure of the effectiveness of natural enemies of diaspine scale insects.–J. econ. Ent. 48, 584–588.CrossRefGoogle Scholar
DeBach, P., Fleschner, C. A. & Dietrick, E. J. (1950). Studies on the efficacy of natural enemies of citrus red mite in southern California.—J. econ. Ent. 43, 807–819.CrossRefGoogle Scholar
Dempster, J. P. (1958). A study of the predators of the broom beetle (Phytodecta olivacea Forster) using the precipitin test.—Proc. R. ent. Soc. Lond. (C) 23, 34.Google Scholar
Dempster, J. P. (1960). A quantitative study of the predators on the eggs and larvae of the broom beetle, Phytodecta olivacea Forster, using the precipitin test.—J. Anim. Ecol. 29, 149–167.CrossRefGoogle Scholar
Dempster, J. P. (1963). The natural prey of three species of Anthocoris (Heteroptera: Anthocoridae) living on broom (Sarothamnus scoparius L.).—Entomologia exp. appl. 6, 149–155.CrossRefGoogle Scholar
Dempster, J. P. (1964). The feeding habits of the Miridae (Heteroptera) living on broom (Sarothamnus scoparius (L.) Wimm.).—Entomologia exp. appl. 7, 149–154.CrossRefGoogle Scholar
Dempster, J. P. (1966). Arthropod predators of the Miridae (Heteroptera) living on broom (Sarothamnus scoparius).—Entomologia exp. appl. 9, 405–412.CrossRefGoogle Scholar
Dempster, J. P. (1967). The control of Pieris rapae with DDT. I. The natural mortality of the young stages of Pieris.—J. appl. Ecol. 4, 485–500.CrossRefGoogle Scholar
Dempster, J. P. (1968). Intra-specific competition and dispersal: as exemplified by a psyllid and its anthocorid predator, pp. 8–17in Southwood, T. R. E. (Ed.). Insect abundance.—160 pp. Oxford, Blackwell Scientific.Google Scholar
Dempster, J. P. (1971). Some effects of grazing on the population ecology of the cinnabar moth (Tyria jacobaeae L.), pp. 517–526 in Duffey, E. & Watt, A. S. (Eds.). The scientific management of animal and plant communities for conservation.—652 pp. Oxford, Blackwell Scientific.Google Scholar
Dempster, J. P., Richards, O. W. & Waloff, N. (1959). Carabidae as predators on the pupal stage of the chrysomelid beetle, Phyrodecta olivacea (Forster).—Oikos 10, 65–70.CrossRefGoogle Scholar
Downe, A. E. R. (1965). Note on the effects of interrupted and multiple feeding on digestion of vertebrate serum proteins in mosquitoes (Diptera: Culicidae).—J. Kansas ent. Soc. 38, 284–286.Google Scholar
Downe, A. E. R. & West, A. S. (1954). Progress in the use of the precipitin test in entomological studies.—Can. Ent. 86, 181–184.CrossRefGoogle Scholar
Eligh, G. S. (1952). Factors influencing the performance of the precipitin test in the determination of blood meals of insects.—Can. J. Zool. 30, 213–218.CrossRefGoogle Scholar
Fleschner, C. A. (1958). Field approach to population studies of tetranychid mites on citrus and avocado in California.—Proc. 10th Int. Congr. Ent. (Montreal, 1956) 2, 669–674.Google Scholar
Fox, C. J. S. & Maclellan, C. R. (1956). Some Carabidae and Staphylinidae shown to feed on a wireworm, Agriotes sputator (L.), by the precipitin test.—Can. Ent. 88, 228–231.CrossRefGoogle Scholar
Frank, J. H. (1967a). The insect predators of the pupal stage of the winter moth, Operophtera brumata (L.) (Lepidoptera: Hydriomenidae).—J. Anim. Ecol. 36, 375–389.CrossRefGoogle Scholar
Frank, J. H. (1967b). A serological method used in the investigation of the predators of the pupal stage of the winter moth, Operophtera brumata (L.) (Hydriomenidae).—Quaest. entomol. 3, 95–105.Google Scholar
Frank, J. H. (1971). Carabidae (Coleoptera) as predators of the red-backed cutworm (Lepidoptera: Noctuidae) in central Alberta.—Can. Ent. 103, 1039–1044.CrossRefGoogle Scholar
Franz, J. M. (1958). The effectiveness of predation and food in limiting gradations of Adelges (Dreyfusia) piceae (Ratz) in Europe.—Proc. 10th Int. Congr. Ent. (Montreal, 1956) 4, 781–787.Google Scholar
Greenstone, M. H. (1977). A passive haemagglutination inhibition assay for the identification of stomach contents of invertebrate predators.—J. appl. Ecol. 14, 457–464.CrossRefGoogle Scholar
Hall, R. R., Downe, A. E. R., Maclellan, C. R. & West, A. S. (1953). Evaluation of insect predator-prey relationships by precipitin test studies.—Mosquito News 13, 199–204.Google Scholar
Harcourt, D. G. (1966). Major factors in survival of the immature stages of Pieris rapae (L.).—Can. Ent. 98, 653–662.CrossRefGoogle Scholar
Healy, J. A. & Cross, T. F. (1975). Immunoelectroosmophoresis for serological identification of predators of the sheep tick Ixodes ricinus.—Oikos 26, 97–101.CrossRefGoogle Scholar
Jenkins, D. W. & Hassett, C. C. (1950). Radio-isotopes in entomology.—Nucleonics 6, 5–14.Google Scholar
Kim, C. W. & Kim, B. K. (1975). Evaluation of the Predators on the larvae of the pine-needle gall midge Thecodiplosis japonensis Uchida et lnouye, by the precipitin test.—Korean J. Ent. 5, 1–5.Google Scholar
Kiritani, K. & Dempster, J. P. (1973). Different approaches to the quantitative evaluation of natural enemies.—.J. appl. Ecol. 10, 323–330.Google Scholar
Kiritani, K., Kawahara, S., Sasaba, T. & Nakasugi, F. (1971). An attempt of rice pest control by integration of pesticides and natural enemies.—Gensei 22, 19–23.Google Scholar
Kupershtein, M. L. (1974). Utilization of the precipitin test for the quantitative estimation of the influence of Pterostichus crenuliger (Coleoptera, Carabidae) upon the population dynamics of Eurygaster integriceps (Hemiptera, Scutelleridae) [In Russian with English summary].—Zool. Zh. 53, 557–562.Google Scholar
Laurell, C. B. (1965). Antigen-antibody crossed electrophoresis.—Analyt. Biochem. 10, 358–361.CrossRefGoogle ScholarPubMed
Leone, C. A. (1947). A serological study of some Orthoptera.—Ann. ent. Soc. Am. 40, 417–433.CrossRefGoogle Scholar
Leone, C. A. (1968). The immunotaxonomic literature: the animal kingdom.—Bull. serol. Mus., New Brunsw. 39, 1–24.Google Scholar
Lock, M. A. & Reynoldson, T. B. (1976). The role of interspecific competition in the distribution of two stream dwelling triclads, Crenobia alpina (Dana) and Polycelis felina (Dalyell), in North Wales.—J. Anim. Ecol. 45, 581–592.CrossRefGoogle Scholar
Loughton, B. G., Derry, C. & West, A. S. (1963). Spiders and the spruce budworm.—Mem. ent. Soc. Can. no. 31, 249–268.CrossRefGoogle Scholar
Loughton, B. G. & West, A. S. (1961). Serological assessment of spider predation on the spruce budworm Choristoneura fumiferana (Clem.) (Lepidoptera: Tortricidae).— Proc. ent. Soc. Ontario 92, 176–180.Google Scholar
Lund, R. D. & Turpin, F. T. (1977). Serological investigation of black cutworm larval consumption by ground beetles.—Ann. ent. Soc. Am. 70, 322–324.CrossRefGoogle Scholar
McFarlane, A. S. (1942). Behaviour of lipoids in human serum.—Nature, Lond. 149, 439.CrossRefGoogle Scholar
MacLellan, C. R. (1973). Natural enemies of the light brown apple moth, Epiphyas postvittana, in the Australian Capital Territory.—Can. Ent. 105, 681–700.CrossRefGoogle Scholar
McLeod, J. H. & Chant, D. A. (1952). Notes on the parasitism and food habits of the european earwig, Forficula auricularia L. (Dermaptera: Forficulidae).—Can. Ent. 84, 343–345.CrossRefGoogle Scholar
Mancini, G., Carbonara, A. O. & Heremans, J. F. (1965). Immunochemical quantitation of antigens by single radial immunodiffusion.—Immunochemistry 2, 235–254.CrossRefGoogle ScholarPubMed
Morris, I. G. (1964). The transmission of antibodies and normal γ-globulins across the young mouse gut.—Proc. R. Soc. (B) 160, 276–292.Google Scholar
Nakamura, M. & Nakamura, K. (1977). Population dynamics of the chestnut gall wasp, Dryocosmus kuriphilus Yasumatsu (Hymenoptera: Cynipidae). V. Estimation of the effect of predation by spiders on the mortality of imaginal wasps based on the precipitin test.—Oecologia (Berlin) 27, 97–116.CrossRefGoogle ScholarPubMed
Newbould, B. B. (1965). Production of allergic encephalomyelitis in rats by injections of spinal chord adjuvant into the inguinal lymph nodes.—Immunology 9, 613–614.Google Scholar
Nishijima, H. (1964). Utilisation of serological methods in entomology [in Japanese].—P1. Prot., Tokyo 18, 429–435.Google Scholar
Nuttall, G. H. F. (1904). Blood immunity and blood relationships.—444 pp. Cambridge University Press.Google Scholar
Oakley, C. L. & Fulthorpe, A. J. (1953). Antigenic analysis by diffusion.–J. Path. Bact. 65, 49–60.CrossRefGoogle ScholarPubMed
Ohlagu, C. E. & Boreham, P. F. L. (1978). A simple field test for evaluating insect prey-predator relationships.—Entomologia exp. appl. 23, 40–47.Google Scholar
O'Rourke, F. J. (1958). Serological tools in entomological research.—Entomologist's Gaz. 9, 63–72.Google Scholar
Ouchterlony, O. (1948). In vitro method for testing toxin producing capacity of diphtheria bacteria.—Acta path, microbial. scand. 25, 186–191.CrossRefGoogle ScholarPubMed
Penney, M. M. (1966). Studies on certain aspects of the ecology of Nebria brevicollis (F.) (Col. Carabidae).—J. Anim. Ecol. 35, 505–512.CrossRefGoogle Scholar
Pettersson, J. (1972). Technical description of a serological method for quantitative predator efficiency studies on Rhopalosiphum padi (L.).—Swedish. agric. Res. 2, 65–69.Google Scholar
Phillipson, J. (1960). The food consumption of different instars of Mitopus morio (F.) (Phalangida) under natural conditions.—J. Anim. Ecol. 29, 299–307.CrossRefGoogle Scholar
Pickavance, J. R. (1970). A new approach to the immunological analysis of invertebrate diets.—J. Anim. Ecol. 39, 715–724.CrossRefGoogle Scholar
Pickavance, J. R. (1971). The diet of the immigrant planarian Dugesia tignina (Girard). II. Food in the wild and comparison with some British species.—J. Anim. Ecol. 40, 637–650.CrossRefGoogle Scholar
Pimentel, D. (1961). Natural control of caterpillar populations on cole crops.—J. econ. Ent. 54, 889–892.CrossRefGoogle Scholar
Pollard, E. (1969). The effect of removal of arthropod predators on an infestation of Brevicoryne brassicae (Hemiptera, Aphididae) on brussels sprouts.—Entomologia exp. appl. 12, 118–124.CrossRefGoogle Scholar
Proom, H. (1943). The preparation of precipitating sera for the identification of animal species.—J. Path. Bact. 55, 419–426.CrossRefGoogle Scholar
Putman, W. L. (1965). Paper chromatography to detect predation on mites.—Can. Ent. 97, 435–441.CrossRefGoogle Scholar
Reeves, W. C., Tempelis, C. H., Bellamy, R. E. & Lofy, M. F. (1963). Observations on the feeding habits of Culex tarsalis in Kern County, California, using precipitating antisera produced in birds.—Am. J. trop. Med. Hyg. 12, 929–935.CrossRefGoogle ScholarPubMed
Reynoldson, T. B. (1964). Evidence for intra-specific competition in field populations of triclads.—J. Anim. Ecol. 33, 187–201.CrossRefGoogle Scholar
Reynoldson, T. B. (1966). The distribution and abundance of lake-dwelling triclads—towards a hypothesis.—Adv. ecol. Res. 3, 1–71.CrossRefGoogle Scholar
Reynoldson, T. B. & Bellamy, L. S. (1971). The establishment of interspecific competition in field populations with an example of competition in action between Polycelis nigra (Mull.) and P. tenuis (Ijima) (Turbellaria, Tricladida), pp. 282–297 in den Boer, P. J. and Gladwell, G. R. (Eds.). Dynamics of populations. Proceedings of the Advanced Study Institute on Dynamics of Numbers in Populations, Oosterbeek, 17–18 09 1970.—611 pp. Wageningen, Centre for Agricultural Publishing and Documentation.Google Scholar
Reynoldson, T. B. & Bellamy, P. (1975). Triclads (Turbellaria: Tricladida) as predators of lake-dwelling stonefly and mayfly nymphs.—Freshwater Biol. 5, 305–312.CrossRefGoogle Scholar
Reynoldson, T. B. & Davies, R. W. (1970). Food niche and co-existence in lake-dwelling triclads.—J. Anim. Ecol. 39, 599–617.CrossRefGoogle Scholar
Reynoldson, T. B. & Sefton, A. D. (1976). The food of Planaria torva (Müller) (Turbellaria: Tricladida), a laboratory and field study.—Freshwater Biol. 6, 383–393.CrossRefGoogle Scholar
Reynoldson, T. B. & Young, J. O. (1963). The food of four species of lake-dwelling triclads. —J. Anim. Ecol. 32, 175–191.CrossRefGoogle Scholar
Richards, O. W. & Waloff, N. (1961). A study of a natural population of Phytodecta olivacea (Forster) (Coleoptera: Chrysomeloidea).—Philos. Trans. R. Soc. Ser. B 244, 205–257.Google Scholar
Robinson, M. H. & Robinson, B. (1970). Prey caught by a sample population of the spider Argiope argentata (Araneae: Araneidae) in Panama: a year's census data.—Zool. J. Linn. Soc. 49, 345–357.CrossRefGoogle Scholar
Rothschild, G. H. L. (1966). A study of a natural population of Conomelus anceps (Germar) (Homoptera: Delphacidae) including observations on predation using the precipitin test.—J. Anim. Ecol. 35, 413–434.CrossRefGoogle Scholar
Rothschild, G. H. L. (1970). Observations on the ecology of the rice-ear bug Leptocorisa oratorius (F.) (Hemiptera: Alydidae) in Sarawak (Malaysian Borneo).—J. appl. Ecol. 7, 147–167.CrossRefGoogle Scholar
Rothschild, G. H. L. (1971). The biology and ecology of rice-stem borers in Sarawak (Malaysian Borneo).—J. appl. Ecol. 8, 287–322.CrossRefGoogle Scholar
Schlein, Y. & Lewis, C. T. (1976). Lesions in haematophagous flies after feeding on rabbits immunised with fly tissues.—Physiol. Ent. 1, 55–59.CrossRefGoogle Scholar
Sergeeva, T. K. (1970a). Methods of obtaining antiserum to protein of Eurygaster integriceps for study of its predators [in Russian with English summary].—Zool. Zh. 49, 440–444.Google Scholar
Sergeeva, T. K. (1970b). Fixing and detecting proteins of Eurygaster integriceps in the gut of predatory arthropods [in Russian with English summary].—Zool. Zh. 49 1715–1720.Google Scholar
Sergeeva, T. K. (1974). Serological observation of predators of the sawfly Neodiprion sertifer (Hymenoptera, Diprionidae) [in Russian with English summary].—Zool. Zh. 53, 710–719.Google Scholar
Sergeeva, T. K. (1975). Cross immunoelectrophoresis as a way of increasing the sensitivity of serological testing when studying trophic relations in insects [in Russian with English summary].—Zool. Zh. 54, 1014–1019.Google Scholar
Service, M. W. (1973a). Mortalities of the larvae of the Anopheles gambiae Giles complex and detection of predators by the precipitin test.—Bull. ent. Res. 62, 359–369.CrossRefGoogle Scholar
Service, M. W. (1973b). Study of the natural predators of Aedes cantans (Meigen) using the precipitin test.—J. med. Ent. 10, 503–510.CrossRefGoogle ScholarPubMed
Service, M. W. (1973c). Identification of predators of Anopheles gambiae resting in huts, by the precipitin test.—Trans. R. Soc. trop. Med. Hyg. 67, 33–34.CrossRefGoogle ScholarPubMed
Service, M. W. (1975). Some predators of Anopheles gambiae larvae in rice fields in Kenya.— Trans. R. Soc. trop. Med. Hyg. 69, 181.Google Scholar
Service, M. W. (1976). Mosquito ecology. Field sampling methods.—583 pp. London, Applied Science Publishers.Google Scholar
Service, M. W. (1977a). Mortalities of the immature stages of species B of the Anopheles gambiae complex in Kenya: comparison between rice fields and temporary pools, identification of predators, and effects of insecticidal spraying.—J. med. Ent. 13, 535–545.CrossRefGoogle ScholarPubMed
Service, M. W. (1977b). Ecological and biological studies on Aedes cantans (Meig.) (Diptera: Culicidae) in southern England.—J. appl. Ecol. 14, 159–196.CrossRefGoogle Scholar
Service, M. W. & Lyle, P. T. W. (1975). Detection of the predators of Simulium damnosum by the precipitin test.—Ann. trop. Med. Parasit. 69, 105–108.CrossRefGoogle ScholarPubMed
Silverman, P. H. (1963). In vitro cultivation and serological techniques in parasitology, pp. 45–67 in Taylor, A. E. R. (ed.). Techniques in parasitology.—107 pp. Oxford, Blackwell scientific.Google Scholar
Smith, H. S. & Debach, P. (1942). The measurement of the effect of entomophagous insects on population densities of their hosts.—J. econ. Ent. 35, 845–849.CrossRefGoogle Scholar
Smith, S. & Silverman, P. H. (1966). Metamorphic antigens of mosquitoes.—Mosquito News 26, 544–551.Google Scholar
Soboleva-Dokuchayeva, I. I. (1973). Study of the trophic relations of Insecta, using the serological method [in Russian].—Usp. sovrem. Biol. 76, 467–476.Google Scholar
Soboleva-Dokuchayeva, I. I. (1975). Determination of carabid (Coleoptera, Carabidae) role in Agrobiological communities by serological method [in Russian with English summary].—Zh. obshch. Biol. 36, 749–761.Google Scholar
Soboleva-Dokuchayeva, I. I. & Podoplelov, I. I. (1972). The study of specific antisera to the soluble proteins of some items of the diet of Carabidae (Col.) [in Russian with English summary].—Zool. Zh. 51, 280–284.Google Scholar
Solomon, M. G. (1973). Ecological studies of grassland leaf hoppers with particular reference to Psammotettix confinis (Dahlbom) (Cicadellidae, Hemiptera).—Ph.D. Thesis, Univ. London.Google Scholar
Southwood, T. R. E. (1966). Ecological methods with particular reference to the study of insect populations.—391 pp. London, Methuen.Google Scholar
Sunderland, K. D. (1975). The diet of some predatory arthropods in cereal crops.—J. appl. Ecol. 12, 507–515.CrossRefGoogle Scholar
Sunderland, K. D. (1977). The population dynamics of Philoscia muscorum (Crustacea, Oniscoidea) in a dune grassland ecosystem with a serological investigation of mortality due to predation.—Ph.D. Thesis, Univ. Leeds.Google Scholar
Sutton, S. L. (1970). Predation on woodlice; an investigation using the precipitin test.—Entomologia exp. appl. 13, 279–285.CrossRefGoogle Scholar
Tay, E. B. (1972). Population studies of Cicadella viridis (L.) And bionomics of Graphocephala coccinea (Forster) (Homoptera, Cicadellidae).—Ph.D. Thesis, Univ. London.Google Scholar
Telfer, W. H. & Williams, C. M. (1953). Immunological studies of insect metamorphism. I. Qualitative and quantitative description of the blood antigens of the cecropia silkworm.—J. gen. Physiol. 36, 389–413.CrossRefGoogle Scholar
Tempelis, C. H. (1975). Host-feeding patterns of mosquitoes, with a review of advances in analysis of blood meals by serology.—J. med. Ent. 11, 635–653.CrossRefGoogle ScholarPubMed
Tempelis, C. H. & Reeves, W. C. (1962). The production of a specific antiserum to bird serum.—Am.J. trop. Med. Hyg. 11, 294–297.CrossRefGoogle ScholarPubMed
Thomas, J. B & Krywienczyk, J (1966). Preliminary results of a serological examination of some Scolytidae and Curculionidae.—Can. Ent. 98, 1094–1099.CrossRefGoogle Scholar
Tinsley, T. W., Robertson, J. S., Rivers, C. F. & Service, M. W. (1971). An iridescent virus of Aedes cantans in Great Britain.—J. invert. Path. 18, 427–428.CrossRefGoogle Scholar
Titova, E. V. (1970). Use of the precipitin test in a study of interrelationships between Eurygaster integriceps Put. (Heteroptera, Scutelleridae) and predatory arthropods [in Russian].—Ent. Obozr. 49, 270–277.Google Scholar
Titova, E. V. (1971). The possibility of using serological methods of investigating the predators of Eurygaster integriceps [in Russian].—Byull. Vses. nauchno-issled. Inst. Zashch. Rast. 20, 50–54.Google Scholar
Titova, E. V. (1973). Assessment of the specificity of the action on the antiserum to the proteins of Eurygaster integriceps put. (Heteroptera, Scutelleridae) [in Russian].—Ent. Obozr. 52, 565–569.Google Scholar
Titova, E. V. (1974). On the time during which the antigens of the prey can be detected in the body of the predator [in Russian].—Ent. Obozr. 53, 726–731.Google Scholar
Titova, E. V. & Kupershtein, M. L. (1976). The Carabidae (Col.) of the wheatfield biocoensis of the steppe zone of Northern Kavkaz (USSR) and assessment of their trophie relations with Eurygaster integriceps (Het. Scutelleridae) by means of the precipitation reaction [in Russian].—Ent. Obozr. 55, 265–276.Google Scholar
Tod, M. E. (1973). Notes on beetle predators of molluscs.—Entomologist 106, 196–201.Google Scholar
Turnbull, A. L. (1960). The prey of the spider Linyphia triangularis (Clerck) (Araneae: Linyphiidae).—Can. J. Zool. 38, 859–873.CrossRefGoogle Scholar
Vickerman, G. P. & Sunderland, K. D. (1975). Arthropods in cereal crops: nocturnal activity, vertical distribution and aphid predation.—J. appl. Ecol. 12, 755–766.CrossRefGoogle Scholar
Watmough, R. H. (1963). Population studies of two species of Psyllidae on broom.—Ph.D. Thesis, Univ. London.Google Scholar
Way, M. J. (1977). Integrated control—practical realities.—Outl. Agric. 9, 127–135.CrossRefGoogle Scholar
Way, M. J. & Banks, C. J. (1958). The control of Aphis fabae Scop. with special reference to biological control of insects that attack annual crops—proc. 10th Int. Congr, Ent. (Montreal, 1956) 4, 907–909.Google Scholar
Weir, D. M. (1973). (Ed.). Handbook of experimental immunology.—2nd edn, 1064 pp. Oxford, Blackwell scientific.Google Scholar
Weitz, B. (1952). The antigenicity of sera of man and animals in relation to the preparation of specific precipitating antisera.—J. Hyg., camb. 50, 275–294.CrossRefGoogle Scholar
Weitz, B. (1956). Identification of blood meals of blood-sucking arthropods.—Bull. Wld Hlth Org. 15, 473–490.Google ScholarPubMed
Weitz, B. (1957). An automatic dispenser for multiple serological titrations.—J. clin. Path. 10, 200–207.CrossRefGoogle ScholarPubMed
Weitz, B. (1960). Feeding habits of bloodsucking arthropods.—Expl. Parasit. 9, 63–82.Google Scholar
West, A. S. (1950). The precipitin test as an entomological tool.—Can. Ent. 82, 241–244.CrossRefGoogle Scholar
West, A. S. (1953). New tools for entomological research—Proc. New Jers. Mosq. Exterm. Ass. 156–163.Google Scholar
West, A. S. (1957). Serological studies at Queen's University.—Bull. Serol. Mus., New Brunsw. 19, 5–6.Google Scholar
West, A. S., Horwood, R. H., Bourns, T. K. R. & Hudson, A. (1959). Systematics of Neodiprion sawflies. I. Preliminary report on serological chromatographic studies—Rep. ent. Soc. Ont. 89, 59–65.Google Scholar
White, G. B., Boreham, P. F. L. & Dolling, W. R. (1972). Synanthropic emesine bugs (Reduviidae, Emesinae) as predators of endophilic mosquitoes.—Trans. R. Soc. trop. Med. Hyg. 66, 535–536.Google ScholarPubMed
Young, J. O. (1973). The prey and predators of Phaenocora typhlops (Vejdovsky) (Turbellaria: Neorhabdocoela) living in a small pond.—J. Anim. Ecol. 42, 637–643.Google Scholar
Young, J. O., Morris, I. G & Reynoldson, T. B. (1964). A serological study of Asellus in the diet of lake-dwelling triclads.—Arch. Hydrobiol. 60, 366–373.Google Scholar