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Simulated field studies with three formulations of Bacillus thuringiensis var. israelensis and Bacillus sphaericus against larvae of Mansonia bonneae (Diptera: Culicidae) in Sarawak, Malaysia

Published online by Cambridge University Press:  10 July 2009

Moh-Seng Chang ,
Beng-Chuan Ho  and
Kai-Lok Chan
Moh-Seng Chang
Affiliation:
Vector Borne Disease Control Department, Medical Department, Sarawak, Malaysia
Beng-Chuan Ho
Affiliation:
Departments of Microbiology & Zoology, National University of Singapore, Kent Ridge, Singapore
Kai-Lok Chan
Affiliation:
Departments of Microbiology & Zoology, National University of Singapore, Kent Ridge, Singapore
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Abstract

The susceptibility levels of Mansonia bonneae Edwards larvae to Bacillus thuringiensis israelensis (B.t.i.) SkeetalR, B.t.i. BactimosR briquettes and Bacillus sphaericus RB80 were evaluated under field conditions. Satisfactory control of larvae was achieved during 24 h–48 h post-treatment. At 72 h after treatment with a 48 h exposure period, over 90% reduction was achieved by SkeetalR at both applied dosages and by B. sphaericus RB80 at the higher applied dosage. BactimosR briquettes achieved less control at both applied dosages, due to slow, sustained release of B.t.i. spores. SkeetalR and B. sphaericus RB80 released their toxic ingredients as early as 2 h after treatment, after which the spore counts (CFU/ml) increased steadily in the middle and bottom water layers and reached maximum level at 72 h post-treatment. However, SkeetalR displayed very little residual activity as evidenced by the drastic decline of spore counts as well as larval reduction at one week post-treatment. By contrast, BactimosR briquette and B. sphaericus RB80 prolonged their residual toxicity throughout the four week period. The peaks of spore counts in the middle and bottom water layers for SkeetalR and B. sphaericus RB80 preceded the corresponding peaks for larval reduction rates by 24 h and 48 h and 1 week to 2 weeks, respectively. The spore counts of BactimosR briquette at the high applied dosage fluctuated closely with the reduction rates of larvae throughout the four week period.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 80 , Issue 2 , June 1990 , pp. 195 - 202
Copyright
Copyright © Cambridge University Press 1990

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References

Berry, W.J., Novak, M.G., Khounlo, S., Rowley, W.A. & Melchior, G.L. (1987) Efficacy of Bacillus sphaericus and Bacillus thuringiensis var. israelensis for control of Culex pipiens and flood water Aedes larvae in Iowa. Journal of the American Mosquito Control Association 3, 579–582.Google Scholar
Chang, M.S. (1987) Simulated field trial of Bacillus thuringiensis var. israelensis (H–14) and Bacillus sphaericus against Mansonia bonneae in swamp forest, Sarawak. Annual Report for National Academy of Science, Washington D.C. (unpublished) 57 pp.Google Scholar
Chang, M.S., Jute, N. & Lah, J. (1988) Breeding-site productivity of the swamp forest mosquitoes Mansonia bonneae and Mansonia dives in Sarawak, East Malaysia. Medical and Veterinary Entomology 2, 19–23.CrossRefGoogle ScholarPubMed
Chow, C.Y. (1953) Pistia clearance with herbicides in rural filariasis control. Bulletin of the World Health Organisation 9, 571–574.Google ScholarPubMed
Chow, C.Y. (1957) Control of Salvinia–a host plant of Mansonia mosquitoes. Bulletin of the World Health Organisation 12, 365–368.Google Scholar
Davidson, E.W., Sweeney, A.W. & Cooper, R. (1981) Comparative field trials of Bacillus sphaericus strain 1593 and B. thuringiensis var. israelensis commercial powder formulations. Journal of Economic Entomology 74, 350–354.CrossRefGoogle Scholar
Davidson, E.W., Urbina, M., Payne, J., Mulla, M.S., Darwazeh, H., Dulmage, H.T. & Correa, J.A. (1984) Fate of Bacillus sphaericus 1593 and 2362 spores used as larvicides in an aquatic environment. Applied Environmental Microbiology 47, 125–129.CrossRefGoogle Scholar
Foo, A.E.S. (1986) Laboratory and field evaluation on the efficacy of Bacillus thuringiensis H–14 for the control of Mansonia and other vector mosquitoes (Diptera: Culicidae), including some comparative studies with Bacillus sphaericus. Ph.D. Thesis, University Sains, Penang, Malaysia, 208 pp.Google Scholar
Foo, A.E.S. & Yap, H.H. (1983) Field trials on the use of Bacillus thuringiensis serotype H–14 against Mansonia mosquitoes in Malaysia. Mosquito News 43, 306–310.Google Scholar
Garcia, R., Federici, B.A., Hall, I.M., Mulla, M.S. & Schaefer, C.H. (1980) BTI–a potent new biological weapon. California Agriculture 34, 18–19.Google Scholar
Golberg, L.J. & Margalit, J. (1977) A bacterial spore demonstrating rapid larvicidal activity against Anopheles sergentii, Uranotaenia unguiculata, Culex univittatus, Aedes aegypti and Culex pipiens. Mosquito News 37, 355–358.Google Scholar
Hambree, S.C., Meisch, M.V. & Williams, D. (1980) Field test of Bacillus thuringiensis var. israelensis against Psorophora columbiae larvae in small rice plots. Mosquito News 40, 67–70.Google Scholar
Lacey, L.A. (1986) Development of operational formulations of Bacillus thuringiensis var. israelensis and Bacillus sphaericus for vector control, pp. 497–500in Samson, R.A., Vlak, J.M. & Peters, D. (Eds) Fundamental and applied aspects of invertebrate pathology. The foundation of the fourth international colloquium of invertebrate pathology, Wageningen, The Netherlands.Google Scholar
Lacey, L.A., Urbina, M.J. & Heizman, C.M. (1984) Sustained release formulations of Bacillus sphaericus and Bacillus thuringiensis (H–14) for control of container-breeding Culex quinquefasciatus. Mosquito News 44, 26–32.Google Scholar
Lee, H.L. & Cheong, W.H. (1987) Field evaluation of the efficacy of Bacillus thuringiensis H–14 for the control of Aedes (Stegomyia) albopictus (Skuse). Mosquito Borne Disease Bulletin 3, 57–63.Google Scholar
Mulla, M.S., Federici, B.A., Darwazeh, H.A. & Ede, L. (1982) Field evaluation of the microbial insecticide Bacillus thuringiensis serotype H–14 against flood water mosquitoes. Applied Environmental Microbiology 43, 1288–1293.CrossRefGoogle Scholar
Mulla, M.S., Darwazeh, H.A., Davidson, E.W. & Dulmage, H.T. (1984a) Efficacy and persistence of the microbial agent Bacillus sphaericus against mosquito larvae in organically enriched habitats. Mosquito News 44, 166–173.Google Scholar
Mulla, M.S., Darwazeh, H.A., Davidson, E.W., Dulmage, H.T. & Singer, S. (1984b) Larvicidal activity and field efficacy of Bacillus sphaericus strains against mosquito larvae and their safety to non-target organisms. Mosquito News 44, 336–342.Google Scholar
Mullen, G.R. & Hinkle, N.C. (1988) Method for determining settling rates of Bacillus thuringiensis serotype H–14 formulation. Journal of the American Mosquito Control Association 4, 132–137.Google Scholar
Mulligan, F.S. III, Schaefer, C.H. & Wilden, W.H. (1980) Efficacy and persistence of Bacillus sphaericus and B. thuringiensis H–14 against mosquitoes under laboratory and field conditions. Journal of Economic Entomology 73, 684–688.CrossRefGoogle Scholar
Pradeep Kumar, N., Sabesan, S., Kuppusamy, M. & Balaraman, K. (1988) Effect of controlled release formulation of Bacillus sphaericus on Mansonia breeding. Indian Journal of Medical Research 87, 15–18.Google Scholar
Silapanuntakul, S., Pantuwatana, S., Bhumiratana, A. & Charoensiri, K. (1983) The comparative persistence of toxicity of Bacillus sphaericus strain 1593 and Bacillus thuringiensis serotype H–14 against mosquito larvae in different kinds of environments. Journal of Invertebrate Pathology 38, 78–87.Google Scholar
Stark, P.M. & Meisch, M.W. (1983) Efficacy of Bacillus thuringiensis serotype H–14 against Psorophora columbiae and Anopheles quadrimaculatus in Arkansas ricelands. Mosquito News 43, 59–62.Google Scholar
Wharton, R.H. (1962) The biology of Mansonia mosquitoes in relation to transmission of filariasis in Malaysia. Bulletin Institute for Medical Research, Malaysia No. 11, 1–114.Google Scholar
Yousten, A.A., Jone, M.E. & Robert, E.B. (1982) Development of selective/differential bacteriological media for the enumeration of Bacillus thuringiensis serovar israelensis (H–14) and Bacillus sphaericus 1593. WHO/VBC/82.844, 7 pp.Google Scholar