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A simple technique for observing and collecting the saliva of tsetse flies (Diptera, Glossinidae)*

Published online by Cambridge University Press:  10 July 2009

Anthony Youdeowei
Affiliation:
The International Centre of Insect Physiology and Ecology, P.O. Box 30772, Nairobi, Kenya

Abstract

Hungry tsetse flies (Glossina morsitans Westw.) are confined individually on the stretched wing membrane of a bat. Only 10–20 s after the tarsi touch the membrane, the flies probe and salivate, the main saliva flow following after penetration. The clear colourless saliva is easily collected onto a microscope slide.

Type
Original Articles
Copyright
Copyright © Cambridge University Press 1975

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References

Bruce, Sir D., Harvey, D., Hamerton, A. E., Davey, J. B. & Bruce, Lady M. E. (1914). Rep. Sleep. Sickn. Commn R. Soc. no. 15, 137, 138, 141.Google Scholar
Burtt, E. (1946). Salivation by Glossina morsitans on to glass slides: a technique for isolating infected flies.—Ann. trop. Med. Parasit. 40, 141144.CrossRefGoogle Scholar
Fairbairn, H. & Williamson, J. (1956). The composition of tsetse-fly saliva. I. A histo-chemical analysis.—Ann. trop. Med. Parasit. 50, 322333.CrossRefGoogle Scholar
Rice, M. J., Kamugisha, C. K. & Sebugwawo, B. S. (1972). Bat's wing membrane for tsetse-fly synthetic feeding.—Trans. R. Soc. trop. Med. Hyg. 66, 328.CrossRefGoogle ScholarPubMed
Williamson, J. (1956). The composition of tsetse-fly saliva. II. Analysis of amino acids and sugars by paper partition chromatography.—Ann. trop. Med. Parasit. 50, 334344.CrossRefGoogle ScholarPubMed