Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-22T18:49:32.393Z Has data issue: false hasContentIssue false
  • English
  • Français

Proteomic research on diapause-related proteins in the female ladybird, Coccinella septempunctata L.

Published online by Cambridge University Press:  25 November 2015

X.Y. Ren ,
L.S. Zhang ,
Y.H. Han ,
T. An ,
Y. Liu ,
Y.Y. Li  and
H.Y. Chen
X.Y. Ren
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences; Sino-American Biological Control Laboratory, USDA-ARS, Beijing 100081, P.R. China
L.S. Zhang*
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences; Sino-American Biological Control Laboratory, USDA-ARS, Beijing 100081, P.R. China
Y.H. Han
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences; Sino-American Biological Control Laboratory, USDA-ARS, Beijing 100081, P.R. China
T. An
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences; Sino-American Biological Control Laboratory, USDA-ARS, Beijing 100081, P.R. China
Y. Liu
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences; Sino-American Biological Control Laboratory, USDA-ARS, Beijing 100081, P.R. China
Y.Y. Li
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences; Sino-American Biological Control Laboratory, USDA-ARS, Beijing 100081, P.R. China
H.Y. Chen
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences; Sino-American Biological Control Laboratory, USDA-ARS, Beijing 100081, P.R. China
*
*Author for correspondence Phone: +86-010-8210-9581 Fax: +86-10-82105926 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

In the experiments reported here, we used the female ladybird Coccinella septempunctata L. as a model to identify diapause-associated proteins using proteomics technology. Our results indicated that protein expression patterns of diapausing and nondiapausing individuals were highly differentiated. A total of 58 spots showed significant differences in abundance (Ratio > 2 and P < 0.05) according to two-dimensional electrophoresis and GE Image Scanner III analysis. Sixteen protein spots were further investigated using mass spectrometry. Eight proteins were characterized, including chaperones and proteins involved in glucose metabolism, lipid metabolism, and the tricarboxylic acid cycle. Among these proteins, five proteins were upregulated in diapausing female adults, including a chaperone (Symbionin symL), malate dehydrogenase (putative), two proteins linked to lipid metabolism (unknown and conserved hypothetical protein) and phosphoglyceromutase (partial). By contrast, isocitrate dehydrogenase (RH49423p), fumarylacetoacetate hydrolase (AGAP001942-PA), and a putative medium chain acyl-CoA dehydrogenase were downregulated. These results contribute to the understanding of diapause mechanisms of the ladybird C. septempunctata and may suggest methods for improving the application of this natural enemy insect.

Keywords

diapause associated proteinsCoccinella septempunctatafemale adultstwo-dimensional electrophoresis
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 106 , Issue 2 , April 2016 , pp. 168 - 174
Check for updates
Copyright
Copyright © Cambridge University Press 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Balazs, R., Machiyama, Y., Hammond, B.J., Julian, T. & Richter, D. (1970) The operation of the y-aminobutyrate bypath of the tricarboxylic acid cycle in brain tissue in vitro . Biochemical Journal 116, 445–467.Google Scholar
Benoit, J.B. & Denlinger, D.L. (2007) Suppression of water loss during adult diapause in the northern house mosquito, Culex pipiens . Journal of Experimental Biology 210, 217–226.CrossRefGoogle ScholarPubMed
Berger, F. (2004) The new life of a centenarian: signalling functions of NAD(P). Trends in Biochemical Sciences 29, 111–118.CrossRefGoogle ScholarPubMed
Bukau, B. & Horwich, A.L. (1998) The Hsp70 and Hsp60 chaperone machines. Cell 92, 351–366.CrossRefGoogle ScholarPubMed
Cho, J.R., Lee, M., Kim, H.S. & Boo, K.S. (2008) Effect of photoperiod and temperature on reproductive diapause of Scotinophara lurida (Burmeister) (Heteroptera: Pentatomidae). Journal of Asia-Pacific Entomology 11, 53–57.CrossRefGoogle Scholar
De Kort, C.A.D. (1990) Thirty-five years of diapause research with the Colorado potato beetle. Entomologia Experimentalis et Applicata 56, 1–13.CrossRefGoogle Scholar
Denlinger, D.L. (2002) Regulation of diapause. Annual Review of Entomology 47, 93–122.CrossRefGoogle ScholarPubMed
Denlinger, D.L. & Armbruster, P.A. (2014) Mosquito diapause. Annual Review of Entomology 59, 73–93.CrossRefGoogle ScholarPubMed
Goward, C.R. & Nicholls, D.J. (1994) Malate dehydrogenase: a model for structure, evolution, and catalysis. Protein Science 3, 1883–1888.CrossRefGoogle Scholar
Hahn, D.A. & Denlinger, D.L. (2007) Meeting the energetic demands of insect diapause: nutrient storage and utilization. Journal of Insect Physiology 53, 760–773.CrossRefGoogle ScholarPubMed
Hahn, D.A. & Denlinger, D.L. (2011) Energetics of insect diapause. Annual Review of Entomology 56, 103–121.CrossRefGoogle ScholarPubMed
Han, Z., Moores, G.D., Denholm, I. & Devoshire, A.L. (1998) Association between biochemical markers and insecticide resistance in the cotton aphid, Aphis gossypii Glover. Pesticide Biochemistry and Physiology 62, 164–171.CrossRefGoogle Scholar
Harvey, W.R. (1962) Metabolic aspects of insect diapasue. Annual Review of Entomology 7, 57–80.CrossRefGoogle Scholar
Hodek, I., & Michaud, J.P. (2008) Why is Coccinella septempunctata so successful (a point-of-view). European Journal of Entomology 105, 1–12.CrossRefGoogle Scholar
Jafri, M.S., Dudycha, S.J. & O'Rourke, B. (2001) Cardiac energy metabolism: models of cellular respiration. Annual Review of Biomedical Engineering 3, 57–81.CrossRefGoogle ScholarPubMed
Jaouannet, M.L., Rodriguez, P.A., Thorpe, P., Lenoir, C.J.G., MacLeod, R., Escudero-Martinez, C. & Bos, J.I.B. (2014) Plant immunity in plant–aphid interactions. Frontiers in Plant Science 5, 1–10.CrossRefGoogle ScholarPubMed
King, A.M. & MacRae, T.H. (2015) Insect heat shock proteins during stress and diapause. Annual Review of Entomology 60, 59–75.CrossRefGoogle ScholarPubMed
Koštál, V. (2006) Eco-physiological phases of insect diapause. Journal of Insect Physiology 52, 113–127.CrossRefGoogle ScholarPubMed
Lehane, M.J. (1997) Peritrophic matrix structure and function. Annual Review of Entomology 42, 525–550.CrossRefGoogle ScholarPubMed
Lees, A.D. (1956) The physiology and biochemistry of diapause. Annual Review of Entomology 1, 1–16.CrossRefGoogle Scholar
Li, A.Q., Popova-Butler, A., Dean, D.H. & Denlinger, D.L. (2007) Proteomics of the flesh fly brain reveals an abundance of upregulated heat shock proteins during pupal diapause. Journal of Insect Physiology 53, 385–391.CrossRefGoogle ScholarPubMed
Lindblad, B., Lindstedt, S. & Steen, G. (1977) On the enzymic defects in hereditary tyrosinemia. Proceedings of the National Academy of Sciences 74, 4641–4645.CrossRefGoogle ScholarPubMed
Lum, P.Y. & Chino, H. (1990) Primary role of adipokinetic hormone in the formation of low density lipophorin in locusts. Journal of Lipid Research 31, 2039–2044.CrossRefGoogle ScholarPubMed
Mayer, M.P. (2010) Gymnastics of molecular chaperons. Molecular Cell 39, 321–331.CrossRefGoogle Scholar
Peter, B., Bogan, K.L. & Brenner, C. (2007) NAD+ metabolism in health and disease. Trends in Biochemical Sciences 32, 12–19.Google Scholar
Puinean, A.M., Foster, S.P., Oliphant, L., Denholm, I., Field, L.M., Millar, N.S., Williamson, M.S. & Bass, C. (2010) Amplification of a cytochrome P450 gene is associated with resistance to neonicotinoid insecticides in the aphid Myzus persicae . Plos Genetics 6, 1–11.CrossRefGoogle ScholarPubMed
Renfree, M.B. & Shaw, G. (2000) Diapause. Annual Review of Entomology 62, 353–375.Google ScholarPubMed
Rinehart, J.P., Li, A.Q., Yocum, G.D., Robich, R.M., Hayward, S.A.L. & Denlinger, D.L. (2007) Up-regulation of heat shock proteins is essential for cold survival during insect diapause. Proceedings of the National Academy of Sciences 104, 11130–11137.CrossRefGoogle ScholarPubMed
Sakurai, H., Goto, K. & Takeda, S. (1983) Emergence of the ladybird beetle, Coccinella septempunctata bruckii Mulsant in the field. Research Bulletin Faculty of Agriculture Gifu University 48, 37–45.Google Scholar
Schwessinger, B., Bart, R., Krasileva, K.V. & Coaker, G. (2015) Focus issue on plant immunity: from model systems to crop species. Frontiers in Plant Science 6, 1–3.CrossRefGoogle ScholarPubMed
Simelane, D.O., Steinkraus, D.C. & Kring, T.J. (2008) Predation rate and development of Coccinella septempunctata L. influenced by Neozygites fresenii-infected cotton aphid prey. Biological Control 44, 128–135.CrossRefGoogle Scholar
St-Louis, M. & Tanguay, R.M. (1997) Mutations in the fmarylacetoacetate hydrolase gene causing hereditary trosinemia type I: overview. Human mutation 9, 291–299.3.0.CO;2-9>CrossRefGoogle ScholarPubMed
Surholt, B., Van, D.J.M., Goldberg, J. & van de Horst, D.J. (1992) Compositional analysis of high- and low-density lipophorin of Acherontia atropos and Locusta migratoria . Biological Chemistry 373, 13–20.Google ScholarPubMed
Tauber, M.J. & Tauber, C.A. (1973) Quantitative response to daylength during diapause in insects. Nature 5414, 296–297.CrossRefGoogle Scholar
Tauber, M.J. & Tauber, C.A. (1976) Insect seasonality: diapause maintenance, termination, and postdiapause development. Annual Review of Entomology 21, 81–107.CrossRefGoogle Scholar
Terra, W.R. (2001) The origin and functions of the insect peritrophic membrane and peritrophic gel. Archives of Insect Biochemistry and Physiology 47, 47–61.CrossRefGoogle ScholarPubMed
True, J.R. (2003) Insect melanism: the molecules matter. Trends in Ecology & Evolution 18, 640–647.CrossRefGoogle Scholar
van Lanteren, J.C. (1988) Biological and integrated pest control in greenhouses. Annual Review of Entomology 33, 239–369.CrossRefGoogle Scholar
van Lenteren, J.C. (2012) The state of commercial augmentative biological control: plenty of natural enemies, but a frustrating lack of uptake. BioControl 57, 1–20.CrossRefGoogle Scholar
Wandinger, S.K., Richter, K. & Buchner, J. (2008) The Hsp90 chaperone machinery. Journal of Biological Chemistry 283, 18473–18477.CrossRefGoogle ScholarPubMed
Wang, W. (2012) Effects of Temperature and Photoperiod on Regulation of Diapause and Post-Diapause Biology in Coccinella septempunctata. Beijing: Chinese Academy of Agricultural Sciences.Google Scholar
Wang, W., Vignani, R., Scali, M. & Cresti, M. (2006) A universal and rapid protocol for protein extraction from recalcitrant plant tissues for proteomic analysis. Electrophoresis 27, 2782–2786.CrossRefGoogle ScholarPubMed
Wang, W., Zhang, L.S., Chen, H.Y., Wang, J., Zhang, J. & Liu, Y. (2013) Effects of temperature and light on diapause induction in lady beetle Coccinella septempunctata in Beijing, China. Chinese Journal of Biological Control 1, 24–30.Google Scholar
Wittkopp, P.J., Carrll, S.B. & Kopp, A. (2003) Evolution in black and white: genetic control of pigment patterns in Drosophila. Trends in Genetics 19, 495–504.CrossRefGoogle ScholarPubMed
Ying, W. (2008) NAD /NADH and NADP /NADPH in cellular functions and cell death: regulation and biological consequences. Antioxidants & Redox Signaling 10, 179–206.CrossRefGoogle ScholarPubMed
Zhang, L.S. (2009) Application of diapause and dormancy in insect culturing. pp. 54–71 in Zeng, F.R. & Chen, H.Y. (Eds) The System Engineering for Rearing Insect Natural Enemies. Beijng, Chinese Agricultural Science and Technology Press.Google Scholar
Zhang, L.S. (2014) The propagation and application of ladybird. pp. 45–87 in Zhang, L.S., Chen, H.Y. & Li, B.P. (Eds) Mass-Rearing and Utilization of Insect Natural Enemies. Beijng, Chinese Agricultural Science and Technology Press.Google Scholar