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Proteins from eggs of the spittlebug Mahanarva spectabilis (Hemiptera: Cercopidae) reveal clues about its diapause regulation

Published online by Cambridge University Press:  27 August 2021

Nayara B. Saraiva ,
Alexander M. Auad ,
Edvaldo Barros ,
Flaviane S. Coutinho ,
Jorge F. Pereira Open the ORCID record for Jorge F. Pereira [Opens in a new window] ,
Rafael A. Barros ,
Humberto J. O. Ramos  and
Maria G. A. Oliveira
Nayara B. Saraiva
Affiliation:
Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, CEP 36570-900, Viçosa, MG, Brazil
Alexander M. Auad
Affiliation:
Embrapa Gado de Leite, CEP 36038-330, Juiz de Fora, MG, Brazil
Edvaldo Barros
Affiliation:
Núcleo de Análise de Biomoléculas, Universidade Federal de Viçosa, CEP 36570-900, Viçosa, MG, Brazil
Flaviane S. Coutinho
Affiliation:
Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, CEP 36570-900, Viçosa, MG, Brazil
Jorge F. Pereira*
Affiliation:
Embrapa Gado de Leite, CEP 36038-330, Juiz de Fora, MG, Brazil
Rafael A. Barros
Affiliation:
Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, CEP 36570-900, Viçosa, MG, Brazil
Humberto J. O. Ramos
Affiliation:
Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, CEP 36570-900, Viçosa, MG, Brazil Núcleo de Análise de Biomoléculas, Universidade Federal de Viçosa, CEP 36570-900, Viçosa, MG, Brazil
Maria G. A. Oliveira
Affiliation:
Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, CEP 36570-900, Viçosa, MG, Brazil
*
Author for correspondence: Jorge F. Pereira, Email: [email protected]
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Abstract

Embryo development in eggs of the spittlebug Mahanarva spectabilis (Distant) (Hemiptera: Cercopidae) passes through four phases (known as S1 to S4) being stopped at S2 during diapause. Studies about the molecular basis of diapause in spittlebugs are nonexistent. Here, we analyzed proteins from non-diapausing (ND), diapausing (D) and post-diapausing (PD) eggs of the spittlebug M. spectabilis. In total, we identified 87 proteins where 12 were in common among the developmental and diapause phases and 19 remained as uncharacterized. Non-diapausing eggs (S2ND and S4ND) showed more proteins involved in information storage and processing than the diapausing ones (S2D). Eggs in post-diapausing (S4PD) had a higher number of proteins associated with metabolism than S2D. The network of protein interactions and metabolic processes allowed the identification of different sets of molecular interactions for each developmental and diapause phases. Two heat shock proteins (Hsp65 and Hsp70) along with two proteins associated with intracellular signaling (MAP4K and a serine/threonine-protein phosphatase) were found only in diapausing and/or post-diapausing eggs and are interesting targets to be explored in future experiments. These results shine a light on one key biological process for spittlebug survival and represent the first search for proteins linked to diapause in this important group of insects.

Keywords

EggdiapauseMahanarva spectabilispost-diapausespittlebug
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 112 , Issue 2 , April 2022 , pp. 219 - 227
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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