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Precocious sexual signalling and mating in Anastrepha fraterculus (Diptera: Tephritidae) sterile males achieved through juvenile hormone treatment and protein supplements

Published online by Cambridge University Press:  29 August 2012

M.C. Liendo ,
F. Devescovi ,
G.E. Bachmann ,
M.E. Utgés ,
S. Abraham ,
M.T. Vera ,
S.B. Lanzavecchia ,
J.P. Bouvet ,
P. Gómez-Cendra  and
J. Hendrichs
...Show all authors
M.C. Liendo
Affiliation:
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina
F. Devescovi
Affiliation:
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina
G.E. Bachmann
Affiliation:
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina
M.E. Utgés
Affiliation:
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (FCEyN, UBA), Argentina
S. Abraham
Affiliation:
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina Facultad de Agronomía y Zootecnia, Universidad Nacional de Tucumán (FAZ, UNT), Argentina
M.T. Vera
Affiliation:
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina Facultad de Agronomía y Zootecnia, Universidad Nacional de Tucumán (FAZ, UNT), Argentina
S.B. Lanzavecchia
Affiliation:
Instituto de Genética ‘E.A. Favret’, INTA, Los Reseros y Nicolás Repetto, 1712 Castelar, Buenos Aires, Argentina
J.P. Bouvet
Affiliation:
Estación Experimental Agropecuaria Concordia, INTA, Argentina
P. Gómez-Cendra
Affiliation:
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (FCEyN, UBA), Argentina
J. Hendrichs
Affiliation:
Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, Insect Pest Control Section, Vienna, Austria
P.E.A. Teal
Affiliation:
Center for Medical, Agricultural, and Veterinary Entomology, US Department of Agriculture – Agricultural Research Service, Florida, USA
J.L. Cladera
Affiliation:
Instituto de Genética ‘E.A. Favret’, INTA, Los Reseros y Nicolás Repetto, 1712 Castelar, Buenos Aires, Argentina
D.F. Segura*
Affiliation:
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina Instituto de Genética ‘E.A. Favret’, INTA, Los Reseros y Nicolás Repetto, 1712 Castelar, Buenos Aires, Argentina
*
*Author for correspondence Fax: +54 11 4450 0805 E-mail: [email protected]
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Abstract

Sexual maturation of Anastrepha fraterculus is a long process. Methoprene (a mimic of juvenile hormone) considerably reduces the time for sexual maturation in males. However, in other Anastrepha species, this effect depends on protein intake at the adult stage. Here, we evaluated the mating competitiveness of sterile laboratory males and females that were treated with methoprene (either the pupal or adult stage) and were kept under different regimes of adult food, which varied in the protein source and the sugar:protein ratio. Experiments were carried out under semi-natural conditions, where laboratory flies competed over copulations with sexually mature wild flies. Sterile, methoprene-treated males that reached sexual maturity earlier (six days old), displayed the same lekking behaviour, attractiveness to females and mating competitiveness as mature wild males. This effect depended on protein intake. Diets containing sugar and hydrolyzed yeast allowed sterile males to compete with wild males (even at a low concentration of protein), while brewer´s yeast failed to do so even at a higher concentration. Sugar only fed males were unable to achieve significant numbers of copulations. Methoprene did not increase the readiness to mate of six-day-old sterile females. Long pre-copulatory periods create an additional cost to the management of fruit fly pests through the sterile insect technique (SIT). Our findings suggest that methoprene treatment will increase SIT effectiveness against A. fraterculus when coupled with a diet fortified with protein. Additionally, methoprene acts as a physiological sexing method, allowing the release of mature males and immature females and hence increasing SIT efficiency.

Keywords

sterile insect techniquesexual maturationmating competitivenesslekking behaviourjuvenile hormonemethoprenenutrition
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 103 , Issue 1 , February 2013 , pp. 1 - 13
Copyright
Copyright © Cambridge University Press 2012

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