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Population structure and genetic diversity analysis in Gynaikothrips uzeli (Zimerman, 1909) (Thysanoptera: Phlaeothripidae) by RAPD markers

Published online by Cambridge University Press:  10 January 2012

R.O. Brito ,
R.F. Artoni ,
M.R. Vicari ,
V. Nogaroto ,
J.C. Silva Jr ,
R.R. Matiello  and
M.C. Almeida
R.O. Brito
Affiliation:
Universidade Estadual de Ponta Grossa, UEPG, Departamento de Biologia Estrutural, Molecular e Genética, Pós-graduação em Biologia Evolutiva, Av. Carlos Cavalcanti, n. 4748, 84030-900 Ponta Grossa, Paraná, Brazil
R.F. Artoni
Affiliation:
Universidade Estadual de Ponta Grossa, UEPG, Departamento de Biologia Estrutural, Molecular e Genética, Pós-graduação em Biologia Evolutiva, Av. Carlos Cavalcanti, n. 4748, 84030-900 Ponta Grossa, Paraná, Brazil
M.R. Vicari
Affiliation:
Universidade Estadual de Ponta Grossa, UEPG, Departamento de Biologia Estrutural, Molecular e Genética, Pós-graduação em Biologia Evolutiva, Av. Carlos Cavalcanti, n. 4748, 84030-900 Ponta Grossa, Paraná, Brazil
V. Nogaroto
Affiliation:
Universidade Estadual de Ponta Grossa, UEPG, Departamento de Biologia Estrutural, Molecular e Genética, Pós-graduação em Biologia Evolutiva, Av. Carlos Cavalcanti, n. 4748, 84030-900 Ponta Grossa, Paraná, Brazil
J.C. Silva Jr
Affiliation:
Universidade Estadual do Sudoeste da Bahia, UESB, Departamento de Ciências Biológicas, Av. José Moreira Sobrinho, s/n, 45200-000, Jequié, Bahia, Brazil
R.R. Matiello
Affiliation:
UEPG, Departamento de Fitotecnia e Fitossanidade, Pós-graduação em Biologia Evolutiva, Av. Carlos Cavalcanti, n. 4748, 84030-900 Ponta Grossa, Paraná, Brazil
M.C. Almeida*
Affiliation:
Universidade Estadual de Ponta Grossa, UEPG, Departamento de Biologia Estrutural, Molecular e Genética, Pós-graduação em Biologia Evolutiva, Av. Carlos Cavalcanti, n. 4748, 84030-900 Ponta Grossa, Paraná, Brazil
*
*Author for correspondence Fax: 55-42-3220-3102 E-mail: [email protected]
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Abstract

Thrips are small insects (0.5–3.0 mm) with distinct habits and life histories characterized by haplodiploid sex determination. In general, low levels of genetic diversity have been reported in haplodiploid insects, although most reports focus on the order Hymenoptera. Therefore, we used RAPD markers to evaluate the structure and both inter- and intra-population genetic variability of Gynaikothrips uzeli (Thysanoptera: Phlaeothripidae). Six populations, three from Paraná state, southern Brazil, and three from Bahia, northeastern Brazil, were studied. Similarly to other haplodiploid insects, the genetic diversity of G. uzeli was reduced. This result is putatively related to the haplodiploid sex determination system, which yields little genetic variation, and to ecological traits of the studied species, such as the low dispersal abilities and life mode in leaf galls. All individuals were homogeneously clustered in their respective collection sites, forming two main groups in which populations from similar environments were more closely related. The analyzed populations were highly structured, and the genetic variation was higher among than within populations.

Keywords

heterozygosityhaplodiploidythripsRAPDgenetic variabilitysimilarity
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 102 , Issue 3 , June 2012 , pp. 345 - 351
Copyright
Copyright © Cambridge University Press 2012

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