Hostname: page-component-78c5997874-j824f Total loading time: 0 Render date: 2024-11-19T17:39:51.006Z Has data issue: false hasContentIssue false
  • English
  • Français

Population structure and genetic diversity analysis in Gynaikothrips uzeli (Zimerman, 1909) (Thysanoptera: Phlaeothripidae) by RAPD markers

Published online by Cambridge University Press:  10 January 2012

R.O. Brito ,
R.F. Artoni ,
M.R. Vicari ,
V. Nogaroto ,
J.C. Silva Jr ,
R.R. Matiello  and
M.C. Almeida
R.O. Brito
Affiliation:
Universidade Estadual de Ponta Grossa, UEPG, Departamento de Biologia Estrutural, Molecular e Genética, Pós-graduação em Biologia Evolutiva, Av. Carlos Cavalcanti, n. 4748, 84030-900 Ponta Grossa, Paraná, Brazil
R.F. Artoni
Affiliation:
Universidade Estadual de Ponta Grossa, UEPG, Departamento de Biologia Estrutural, Molecular e Genética, Pós-graduação em Biologia Evolutiva, Av. Carlos Cavalcanti, n. 4748, 84030-900 Ponta Grossa, Paraná, Brazil
M.R. Vicari
Affiliation:
Universidade Estadual de Ponta Grossa, UEPG, Departamento de Biologia Estrutural, Molecular e Genética, Pós-graduação em Biologia Evolutiva, Av. Carlos Cavalcanti, n. 4748, 84030-900 Ponta Grossa, Paraná, Brazil
V. Nogaroto
Affiliation:
Universidade Estadual de Ponta Grossa, UEPG, Departamento de Biologia Estrutural, Molecular e Genética, Pós-graduação em Biologia Evolutiva, Av. Carlos Cavalcanti, n. 4748, 84030-900 Ponta Grossa, Paraná, Brazil
J.C. Silva Jr
Affiliation:
Universidade Estadual do Sudoeste da Bahia, UESB, Departamento de Ciências Biológicas, Av. José Moreira Sobrinho, s/n, 45200-000, Jequié, Bahia, Brazil
R.R. Matiello
Affiliation:
UEPG, Departamento de Fitotecnia e Fitossanidade, Pós-graduação em Biologia Evolutiva, Av. Carlos Cavalcanti, n. 4748, 84030-900 Ponta Grossa, Paraná, Brazil
M.C. Almeida*
Affiliation:
Universidade Estadual de Ponta Grossa, UEPG, Departamento de Biologia Estrutural, Molecular e Genética, Pós-graduação em Biologia Evolutiva, Av. Carlos Cavalcanti, n. 4748, 84030-900 Ponta Grossa, Paraná, Brazil
*
*Author for correspondence Fax: 55-42-3220-3102 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Thrips are small insects (0.5–3.0 mm) with distinct habits and life histories characterized by haplodiploid sex determination. In general, low levels of genetic diversity have been reported in haplodiploid insects, although most reports focus on the order Hymenoptera. Therefore, we used RAPD markers to evaluate the structure and both inter- and intra-population genetic variability of Gynaikothrips uzeli (Thysanoptera: Phlaeothripidae). Six populations, three from Paraná state, southern Brazil, and three from Bahia, northeastern Brazil, were studied. Similarly to other haplodiploid insects, the genetic diversity of G. uzeli was reduced. This result is putatively related to the haplodiploid sex determination system, which yields little genetic variation, and to ecological traits of the studied species, such as the low dispersal abilities and life mode in leaf galls. All individuals were homogeneously clustered in their respective collection sites, forming two main groups in which populations from similar environments were more closely related. The analyzed populations were highly structured, and the genetic variation was higher among than within populations.

Keywords

heterozygosityhaplodiploidythripsRAPDgenetic variabilitysimilarity
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 102 , Issue 3 , June 2012 , pp. 345 - 351
Copyright
Copyright © Cambridge University Press 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Baumann, R., Schubert, R., Heitland, W., Auger-Rozenberg, M.-A., Faivre-Rimpant, P. & Müller-Starck, G. (2003) Genetic diversity within and among populations of Diprion pini (Hym., Diprionidae) determined by random amplified polymorphic DNA-polymerase chain reaction of haploid males. Journal of Applied Entomology 127, 258–264.Google Scholar
Bayar, K., Törjék, O., Kiss, E., Gyulai, G. & Heszky, L. (2002) Intra- and interspecific molecular polymorphism of thrips species. Acta Biologica Hungarica 53, 317–324.CrossRefGoogle ScholarPubMed
Bertorelle, G., Bucchini, L., Pilastro, A. & Matessi, C. (1999) DNA fingerprinting data and the analysis of population genetic structure by comparing band-sharing patterns. Molecular Ecology 8, 1851–1866.CrossRefGoogle ScholarPubMed
Boato, A. & Battisti, A. (1996) High genetic variability despite haplodiploidy in primitive sawflies of the genus Cephalcia (Hymenoptera, Pampilidae). Experientia 52, 516–521.CrossRefGoogle Scholar
Boraschi, D. & Del Lama, M.A. (2004) Populational genetic structure and sociogenetic structure of cocoon masses of Digelasinus diversipes (Kirby, 1882) (Hymenoptera: Symphyta: Argidae). Genetic and Molecular Biology 27, 385–390.CrossRefGoogle Scholar
Brunner, P.C. & Frey, J.E. (2010) Habitat-specific population structure in native western flower thrips Frankliniella occidentalis (Insecta: Thysanoptera). Journal of Evolutionary Biology 23, 797–804.Google Scholar
Brunner, P.C., Fleming, C. & Frey, E. (2002) A molecular key for economically important thrips species (Thysanoptera: Thripidae) using direct sequencing and a PCR-RFLP-based approach. Agricultural and Forest Entomology 4, 127–138.Google Scholar
Callejas, C., Beitia, F.J., Gobbi, A., Velasco, A. & Ochando, M.D. (2005) The use of RAPD markers to detect genetic patterns in Aleurodicus disperses (Hemiptera: Aleyrodidae) populations from the Canary Islands. European Journal of Entomology 102, 289–291.Google Scholar
Crespi, B.J. (1991) Heterozygosity in the haplodiploid Thysanoptera. Evolution 45, 458–464.Google Scholar
Crespi, B.J. (1993) Sex allocation ratio selection in Thysanoptera. pp. 214–234 in Wrensch, D.L. & Ebbert, M.A. (Eds) Evolution and Diversity of Sex Ratio in Insects and Mites. New York, USA, Chapman Hall.CrossRefGoogle Scholar
Excoffier, L. & Lischer, H.E.L. (2010) Arlequin Suite ver 3.5: A New Series of Programs to Perform Population Genetics Analyses Under Linux and Windows. Molecular Ecology Resources 10, 564–567.CrossRefGoogle ScholarPubMed
Excoffier, L., Smouse, P.E. & Quattro, J.M. (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitocondrial DNA restriction data. Genetics 131, 479–491.Google Scholar
Fuchs, H., Gross, R., Stein, H. & Rottmann, O. (1998) Application of molecular genetic markers for the differentiation of bream (Abramis brama L.) populations from the rivers Main and Danube. Journal of Applied Ichthyology 14, 49–55.CrossRefGoogle Scholar
Hartl, D.L. (1971) Some aspects of natural selection in Arrhenotokous populations. American Zoologist 11, 305–325.Google Scholar
Hedrick, P.W. & Parker, J.D. (1997) Evolutionary genetics and genetic variation of Haplodiploids and X-linked genes. Annual Review of Ecology, Evolution, and Systematics 28, 55–83.Google Scholar
IBGE (2004) Mapa de climas do Brasil, 1a aproximação (1:5.000.000). Série mapas murais. Ministério do Meio Ambiente, Ministério do Planejamento, Orçamento e Gestão, IBGE, Diretoria de Geociências. Available online at http://www.ibge.gov.br/home/geociencias/default_prod.shtm#GEOG/ (accessed 3 September 2010).Google Scholar
Jain, S.K., Neekhra, B., Pandey, D.K. & Jain, K. (2010) RAPD Markers System in Insect Study: A review. Indian Journal of Biotechnology 9, 7–12.Google Scholar
Kerr, W.E. (1976) Population genetics studies in bees. II. Sex-limited genes. Evolution 30, 94–99.Google Scholar
Kumm, S. & Moritz, G. (2008) First detection of Wolbachia in arrhenotokous populations of thrips species (Thysanoptera: Thripidae and Phlaeothripidae) and its role in reproduction. Environmental Entomology 37, 1422–1428.Google Scholar
Lewontin, R.C. (1972) The apportionment of the human diversity. Evolutionary Biology 6, 381–398.Google Scholar
Loxdale, H.D. & Lushai, G. (1998) Molecular markers in entomology (review). Bulletin of Entomological Research 88, 577–600.Google Scholar
Metcalf, R.A., Marlin, J.C. & Whitt, G.S. (1975) Low levels of genetic heterozygosity in Hymenoptera. Nature 257, 792–793.CrossRefGoogle ScholarPubMed
Mound, L.A. (2005) Thysanoptera: Diversity and interactions. Annual Review of Entomology 50, 247–269.Google Scholar
Mound, L.A. & Kibby, G. (1998) Thysanoptera: An Identification Guide. 2nd edn. Wallingford, UK, CAB International.Google Scholar
Mound, L.A. & Marullo, R. (1996) The Thrips of Central and South America: An Introduction (Insecta: Thysanoptera). Gainesville, FL, USA, Associated Publishers.Google Scholar
Mound, L.A. & Morris, D.C. (2007) The insect order Thysanoptera: classification versus systematics. Zootaxa 1668, 395–411.Google Scholar
Nei, M. (1973) Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences 70, 3321–3323.Google Scholar
Pamilo, P., Vepsalainen, K. & Rosengren, R. (1975) Low allozymic variability in Formica ants. Hereditas 80, 293–296.Google Scholar
Roberts, D.B. (1998) Drosophila: A Practical Approach. Oxford, UK, IRL Press at Oxford University Press.Google Scholar
Rohlf, F.J. (2000) NTSYS-pc: numerical taxonomy and multivariate analysis system, version 2.1. New York, USA, Exeter Software.Google Scholar
Rosenmeier, L. & Packer, L. (1993) A comparison of genetic variation in two sibling species pairs of haplodiploid insects. Biochemical Genetics 31, 185–200.CrossRefGoogle ScholarPubMed
Sneath, P.H. & Sokal, R.R. (1973) Numerical Taxonomy: The Principles and Practice of Numerical Classification. San Francisco, CA, USA, W.H. Freeman.Google Scholar
Snyder, T.P. (1974) Lack of allozymic variability in three bee species. Evolution 28, 687–689.Google Scholar
Sunnucks, P. (2000) Efficient genetic markers for population biology. Trends in Ecology and Evolution 15, 199–203.Google Scholar
Terry, I. (1997) Host selection, communication and reproductive behaviour. pp. 65–118 in Lewis, T. (Ed.) Thrips as Crop Pests. New York, USA, CAB International Oxon.Google Scholar
Van Valen, L. (1965) Morphological variation and width of ecological niche. American Naturalist 99, 377–390.Google Scholar
Waldschmidt, A.M., Marco-Junior, P., Barros, E.G. & Campos, L.A.O. (2000) Genetic analysis of Melipona quadrifasciata LEP (Hymenoptera: Apidae, Meliponinae) with RAPD markers. Brazilian Journal of Biology 62, 923–928.Google Scholar
Welsh, J. & Mcclelland, M. (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Research 18, 7213–7218.CrossRefGoogle ScholarPubMed
Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. & Tingey, S.V. (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18, 6531–6535.Google Scholar
Yapi, I. & Nelson, R.J. (1996) WinBoot: a program for performing bootstrap analysis of binary data to determine the confidence limits of UPGMA-based dendrograms. Manila, Philippines, IRRI.Google Scholar
Yeh, F.C., Yang, R.C. & Boyle, T. (1999) PopGene (version 1.32). Microsoft window-base software for population genetic analysis: a quick user's guide. Alberta, Canada, University of Alberta, Center for International Forestry Research.Google Scholar