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Organophosphorus resistance in the sheep blowfly, Lucilia cuprina (Wiedemann) (Diptera: Calliphoridae): a genetic study incorporating synergists

Published online by Cambridge University Press:  10 July 2009

P. B. Hughes
P. B. Hughes
Affiliation:
Biological and Chemical Research Institute, Department of Agriculture, New South Wales, P.M.B. 10, P.O., Rydalmere 2116, Australia
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Abstract

Pre-treatment of females of Lucilia cuprina (Wied.) with the synergist piperonyl butoxide increased the resistance factors of an organophosphorus-resistant strain Q to four organophosphates in comparison to the susceptible strain LS. However, pre-treatment with S,S,S-tributylphosphorothrithioate (TBPT) reduced the resistance factors of Q to three organophosphorus insecticides. As TBPT is an esterase inhibitor, it is suggested that esterases contribute to resistance in the Q strain. Resistance to diazinon in the Q strain is due to a single, partially-dominant gene on chromosome 4. By the use of the backcross and discriminating dose technique, resistance to parathion, fenitrothion, chlorfenvinphos and fenitro-oxon (dimethyl 3-methyl-4-nitrophenyl phosphate) (the latter two were synergised by piperonyl butoxide to obtain a genetically discriminating dose) was also shown to be controlled by a single, partially-dominant gene on chromosome 4. It is concluded that this gene confers resistance to organophosphorus compounds in L. cuprina, probably by providing an esterase with enhanced ability to degrade organophosphates.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 72 , Issue 4 , December 1982 , pp. 573 - 582
Copyright
Copyright © Cambridge University Press 1982

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