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Nuclear DNA content of the whitefly Bemisia tabaci (Aleyrodidae: Hemiptera) estimated by flow cytometry

Published online by Cambridge University Press:  09 March 2007

J.K. Brown ,
G.M. Lambert ,
M. Ghanim ,
H. Czosnek  and
D.W. Galbraith
J.K. Brown*
Affiliation:
Department of Plant Sciences, The University of Arizona, Tucson, AZ 85721, USA
G.M. Lambert
Affiliation:
Department of Plant Sciences, The University of Arizona, Tucson, AZ 85721, USA
M. Ghanim
Affiliation:
Department of Entomology, Agricultural Research Organization, Bet Dagan, Israel
H. Czosnek
Affiliation:
The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot 76100, Israel
D.W. Galbraith
Affiliation:
Department of Plant Sciences, The University of Arizona, Tucson, AZ 85721, USA
*
*Fax: ++1-520-621-8839 E-mail: [email protected]
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Abstract

The nuclear DNA content of the whitefly Bemisia tabaci (Gennnadius) was estimated using flow cytometry. Male and female nuclei were stained with propidium iodide and their DNA content was estimated using chicken red blood cells and Arabidopsis thaliana L. (Brassicaceae) as external standards. The estimated nuclear DNA content of male and female B. tabaci was 1.04 and 2.06 pg, respectively. These results corroborated previous reports based on chromosome counting, which showed that B. tabaci males are haploid and females are diploid. Conversion between DNA content and genome size (1 pg DNA = 980 Mbp) indicate that the haploid genome size of B. tabaci is 1020 Mbp, which is approximately five times the size of the genome of the fruitfly Drosophila melanogaster Meigen. These results provide an important baseline that will facilitate genomics-based research for the B. tabaci complex.

Keywords

Bemisia tabacinuclear DNAgenomeflow cytometryHemiptera
Type
Research Article
Information
Bulletin of Entomological Research , Volume 95 , Issue 4 , July 2005 , pp. 309 - 312
Copyright
Copyright © Cambridge University Press 2005

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