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Multiplex PCR assay to discriminate four neighbouring species of the Calliptamus genus (Orthoptera: Acrididae) from France

Published online by Cambridge University Press:  27 May 2010

E. Blanchet ,
L. Blondin ,
P.A. Gagnaire ,
A. Foucart ,
J.M. Vassal  and
M. Lecoq
E. Blanchet*
Affiliation:
CIRAD Acridologie, Montpellier, F-34398France
L. Blondin
Affiliation:
CIRAD Acridologie, Montpellier, F-34398France
P.A. Gagnaire
Affiliation:
Institut des Sciences de l'Evolution, UMR 5554, Université Montpellier 2, cc065, place E. Bataillon, 34095Montpellier cedex 05, France
A. Foucart
Affiliation:
CIRAD Acridologie, Montpellier, F-34398France
J.M. Vassal
Affiliation:
CIRAD Acridologie, Montpellier, F-34398France
M. Lecoq
Affiliation:
CIRAD Acridologie, Montpellier, F-34398France
*
*Author for correspondence Fax: +33 (0)4 67 59 38 73 E-mail: [email protected]
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Abstract

Definition of the genus Calliptamus (Orthoptera: Acrididae) has generated many taxonomic debates. Even now, the existence of different geographical morphs hinders species determination, particularly as concerns females and larvae. Some of these species are observed in southern France and are recognized as potential pests. To circumvent problems of species identification in ecological surveys, we developed a single multiplex PCR method based on mitochondrial Cytochrome Oxydase I diagnostic polymorphisms to differentiate between the four species, Calliptamus italicus, C. wattenwylianus, C. siciliae and C. barbarus, in southern regions of France.

Keywords

Calliptamusspecies identificationmolecular diagnosticsmitochondrial Cytochrome Oxydase I
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 100 , Issue 6 , December 2010 , pp. 701 - 706
Copyright
Copyright © Cambridge University Press 2010

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