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Molecular characterisation and developmental expression analysis of the 5-HT7 receptor in Chrysopa formosa

Published online by Cambridge University Press:  18 March 2025

Fei Li Open the ORCID record for Fei Li [Opens in a new window] ,
Longrui Wang ,
Yujia Li ,
Xuyang Tian ,
Jiayue Wang ,
Zhongjian Shen ,
Junjie Zhang ,
Lisheng Zhang  and
Yuyan Li Open the ORCID record for Yuyan Li [Opens in a new window]
Fei Li
Affiliation:
Engineering Research Center of Natural Enemies, Jilin Province International Cooperation Key Laboratory for Biological Control of Agricultural Pests, Institute of Biological Control, Jilin Agricultural University, Changchun, PR China State Key Laboratory for Biology of Plant Diseases and Insect Pests, Key Laboratory of Natural Enemy Insects, Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China
Longrui Wang
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Key Laboratory of Natural Enemy Insects, Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China
Yujia Li
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Key Laboratory of Natural Enemy Insects, Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China
Xuyang Tian
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Key Laboratory of Natural Enemy Insects, Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China
Jiayue Wang
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Key Laboratory of Natural Enemy Insects, Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China
Zhongjian Shen
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Key Laboratory of Natural Enemy Insects, Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China
Junjie Zhang*
Affiliation:
Engineering Research Center of Natural Enemies, Jilin Province International Cooperation Key Laboratory for Biological Control of Agricultural Pests, Institute of Biological Control, Jilin Agricultural University, Changchun, PR China
Lisheng Zhang
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Key Laboratory of Natural Enemy Insects, Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China Key Laboratory of Animal Biosafety Risk Prevention and Control (North) of Ministry of Agriculture and Rural Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, PR China
Yuyan Li*
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Key Laboratory of Natural Enemy Insects, Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China
*
Corresponding author: Yuyan Li; Email: [email protected]; Junjie Zhang; Email: [email protected]
Corresponding author: Yuyan Li; Email: [email protected]; Junjie Zhang; Email: [email protected]
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Abstract

Serotonin (5-hydroxytryptamine, 5-HT) is a key monoamine neurotransmitter in insects, which regulates neural functions and influences various developmental and physiological processes by binding to its receptors. In this study, we investigate the molecular characteristics, phylogenetic relationships, and expression patterns of the 5-HT7 receptor (Cf5-HT7) in Chrysopa formosa, with a focus on its potential involvement in developmental and diapause regulation. The Cf5-HT7 gene was identified and cloned from the C. formosa transcriptome, revealing an open reading frame of 1788 bp encoding a 596 amino acid protein. Sequence analysis confirmed that Cf5-HT7 is a typical class A G protein-coupled receptor, characterised by seven transmembrane domains and several post-translational modifications, including palmitoylation and N-glycosylation sites. Phylogenetic analysis revealed that Cf5-HT7 is most closely related to the 5-HT7 receptor from Chrysoperla carnea, with high conservation of key motifs involved in ligand binding and receptor activation. Expression analysis across different developmental stages of C. formosa showed that Cf5-HT7 is highly expressed in the first instar larvae, with significant upregulation observed during the prepupal stage. Under diapause-inducing conditions, Cf5-HT7 expression is modulated in a stage-specific manner, showing a marked decrease at the onset of diapause, followed by a significant increase during the mid-to-late diapause maintenance phase. These findings suggest that it plays a pivotal role in regulating development and diapause processes in C. formosa, offering new insights into the molecular mechanisms governing insect life cycle transitions. This study lays the groundwork for future research into the functional roles of 5-HT7 receptors in insect physiology and their potential applications in manipulating diapause.

Keywords

5-HT7 receptordiapauseexpression patternlacewingserotonin
Type
Research Paper
Information
Bulletin of Entomological Research , First View , pp. 1 - 12
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Copyright
© The Author(s), 2025. Published by Cambridge University Press.

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