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Molecular and morphological variability within the Aphidius colemani group with redescription of Aphidius platensis Brethes (Hymenoptera: Braconidae: Aphidiinae)

Published online by Cambridge University Press:  12 May 2014

Ž. Tomanović ,
A. Petrović ,
M. Mitrović ,
N.G. Kavallieratos ,
P. Starý ,
E. Rakhshani ,
M. Rakhshanipour ,
A. Popović ,
A.H. Shukshuk  and
A. Ivanović
Ž. Tomanović
Affiliation:
Faculty of Biology, Institute of Zoology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia
A. Petrović*
Affiliation:
Faculty of Biology, Institute of Zoology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia
M. Mitrović
Affiliation:
Department of Plant Pests, Institute for Plant Protection and Environment, Banatska 33, 11080 Zemun, Serbia
N.G. Kavallieratos
Affiliation:
Laboratory of Agricultural Entomology, Department of Entomology and Agricultural Zoology, Benaki Phytopathological Institute, 8 Stefanou Delta str., Kifissia, 14561, Attica, Greece
P. Starý
Affiliation:
Laboratory of Aphidology, Institute of Entomology, Biology Centre of the Czech Academy of Sciences, Branišovská 31, 37005 České Budějovice, Czech Republic
E. Rakhshani
Affiliation:
Department of Plant Protection, College of Agriculture, Zabol University, P.O.Box: 998615-538, Zabol, I.R. Iran
M. Rakhshanipour
Affiliation:
Faculty of Basic Science, University of Zabol, P.O.Box: 998615-538, Zabol, I.R.Iran
A. Popović
Affiliation:
Faculty of Biology, Institute of Zoology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia
A.H. Shukshuk
Affiliation:
Elmergib University Faculty of Arts and Sciences, Zliten, Libya
A. Ivanović
Affiliation:
Faculty of Biology, Institute of Zoology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia
*
*Author for correspondence Phone: +381 11 2187 266 Fax: +381 11 2638 500 E-mail: [email protected]
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Abstract

We have identified the following three taxa related to the Aphidius colemani species group, which are important biological control agents: Aphidius colemani, Aphidius transcaspicus and Aphidius platensis. Using partial sequences of the mitochondrial cytochrome oxidase subunit I (mtCOI) gene and geometric morphometric analysis of the forewing shape, we have explored the genetic structure and morphological variability of the A. colemani group from different aphid host/plant associations covering a wide distribution area. The topology of the maximum parsimony and maximum likelihood trees were identical with 98–100% bootstrap support, clustering A. colemani, A. platensis and A. transcaspicus into separate species. The distances among the taxa ranged from 2.2 to 4.7%, which is a common rate for the between-species divergence within the subfamily Aphidiinae. Differences in the shape of the forewing investigated within the biotypes of A. colemani group are congruent with their genetic diversification. Both A. platensis and A. colemani share a common host range pattern, and it would be interesting to estimate and compare the role of these two species in future biological control strategies against aphids of economic importance. Our results indicate that ‘genetic screening’ is a reliable approach for identification within the A. colemani group. The high variation in the wing shape among species, including a significant divergence in the wing shape among specimens that emerged from different hosts, makes the forewing shape and wing venation less reliable for species determination. Aphidius platensis is diagnostified and redescribed, and the key for the A. colemani group is presented.

Keywords

aphid parasitoidsAphidiusmtCOI barcodinggeometric morphometrics
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 104 , Issue 5 , October 2014 , pp. 552 - 565
Copyright
Copyright © Cambridge University Press 2014 

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