To permit detailed study of individual tsetse, either as insects or as the vectors of trypanosomiasis, a method has been devised which resulted in excellent survival and reproduction when tested on females or Glossina austeni Newst.

Each mated female was maintained in asmall cage comprising a stainless steel frame covered with non-absorbent black Terylene netting through which the larvae and faeces fell into a tray. Each cage was applied daily to the ear of a rabbit, and records were kept of the number of feeds, time taken to feed, numbers of larvae produced, lengths of interlarvel periods and weights of pupae.

The following conclusions are tentative as they are based on detailed studies of only eight females. Five of these produced larvae regularly at 8½- to 9-day intervals. Ninety-six interlarval periods were investigated. Although the mean interval between meals was two days, the flies tended to fast before larviposition and to engorge repeatedly afterwards.

Among nearly regular breeders, failure to larviposit did not seem to interrupt the normal feeding rhythum: the reproductive rhythum later re-integrated with the feeding ehythum. A tendency to deviate from the normal feeding pattern either by too frequent feeding after larviposition or by prologation of the fasting period before larviposition, may possible be related to pemature death, but flies which tend to feed on the day before larviposition centainly risk death from pupation in utero—a condition found in 5 per cent. of dead rabbit-fed flies; the complex of pressures which lead to this condition is described.

Limited data suggest that females which live to a great age enter a post-productive period when about 6½ month old; the feeding pattern breaks down completely, the fly fasting for periods of up to 11 days, or even longer in the immediate pre-death period. The maximum output of reasonably sized pupae is probably about 20 for G. austeni.

Pupal weight increased until the parent flies were about 90 days old, but there was much individual variation; thereafter the weight remained high, but among very old dlies it fell towards the end of reproductive life. Certain individuals tended to produce heavy pupae, a characteristic probable associated with the size of the fly and the total weight of blood taken up in each interlarval period—not with the frequency of feeding; a very infrequent feeder may, however, produce underweight pupae at irregular intervals.