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A method for the rapid recognition of nulliparous and parous females of haematophagous Diptera

Published online by Cambridge University Press:  10 July 2009

T.Q. Hoc
T.Q. Hoc*
Affiliation:
Lehrstuhl für Spezielle Zoologie und Parasitologie, Ruhr-Universität Bochum, Germany
*
Faculty of Biology, The State University of Hanoi, 90 Nguyen Trai Road, Thvong Dinh, Dong Da, Hanoi, Vietnam.
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Abstract

Based on the fact that in haematophagous Diptera, the basal bodies, groups of six to nine specialized epithelial cells in the calyx wall within the ovariolar sheath, become granular after ovulation, a method for rapidly determining parity of the females has been developed. After supravitally staining intact ovaries with fresh neutral red solution in physiological saline (1:3000–1:5000) for one to three minutes, granular basal bodies in parous females can be clearly recognized under a stereo-microscope or a compound microscope with a raised condensor. The advantages of the method are its speed, accuracy and simplicity.

Type
Research Article
Information
Bulletin of Entomological Research , Volume 86 , Issue 2 , April 1996 , pp. 137 - 141
Copyright
Copyright © Cambridge University Press 1996

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