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Malathion resistance conferred by a carboxylesterase in Anopheles culicifacies Giles (species B) (Diptera: Culicidae)

Published online by Cambridge University Press:  10 July 2009

C. A Malcolm  and
R. G. Boddington
C. A Malcolm
Affiliation:
London School of Hygiene and Tropical Medicine, Keppel Street, Gower Street, London, WC1E 7HT, UK
R. G. Boddington
Affiliation:
London School of Hygiene and Tropical Medicine, Keppel Street, Gower Street, London, WC1E 7HT, UK
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Abstract

A strain of Anopheles culicifaciesGiles (species B) originally collected from Aurangabad, Maharashtra State, India, showed highly specific resistance to malathion. Homogenates of one-day-old adults metabolized 14C-labelled malathion at a level comparable with strains of A. stephensi Liston and A. arabiensis Patton known to possess the malathion carboxylesterase resistance mechanism and at a higher rate (6.4 and 7.8 times, respectively) than malathion-susceptible strains of these species. The major metabolite obtained was malathion dicarboxylic acid, a carboxylesterase product, which suggests that this may be the principal resistance mechanism in this strain. Levels of non-specific esterase activity in larvae and adults were similar to those shown by both the susceptible and resistant strains of A. stephensi, implying that no quantitative increase in esterases had occurred. Microtitre plate assays on individual mosquitoes provided no evidence for the presence of the insensitive acetylcholinesterase resistance mechanism.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 79 , Issue 2 , June 1989 , pp. 193 - 199
Copyright
Copyright © Cambridge University Press 1989

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