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Laboratory colonization of the tsetse fly Glossina pallidipes Austen (Diptera: Glossinidae) using an in vitro feeding method

Published online by Cambridge University Press:  10 July 2009

P. A. Langley
P. A. Langley
Affiliation:
Overseas Development Administration/University of Bristol, Tsetse Research Laboratory, Langford, Bristol, BS18 7DU, UK
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Abstract

Attempts to establish a laboratory colony of Glossina pallidipes Austen from adults and puparia of Uganda origin (Lugala) sent from a colony previously established in Amsterdam have been highly successful. Flies are maintained at 25 ± 0·5°C, L:D 12:12 and 85% RH. They are fed six days per week on whole defibrinated pig blood collected aseptically and irradiated at 1·5 kGy before storage at 4°C. The blood is presented through silicon rubber membranes at 37°C. Virgin female flies are mated when 7–9 days old with equal numbers of 9–12-day-old males in a 30 × 30 × 10-cm cage covered with white Terylene netting and illuminated with a fluorescent light from above, providing 300–500 lux on the cage bottom. The sexes are left together for 40–48 h, during which time they are offered food twice. After separation of the sexes, 30 females are housed in a cage 15 cm in diameter and two males are added. Flies are kept for 90 days before discarding. The establishment of this colony is discussed in terms of the mating behaviour of the species, highlighting differences in the laboratory between G. pallidipes from Uganda and Zimbabwe and between G. pallidipes and G. morsitans morsitans Westwood.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 79 , Issue 3 , September 1989 , pp. 429 - 435
Copyright
Copyright © Cambridge University Press 1989

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References

Foster, W. A. (1976). Male sexual maturation of the tsetse flies Glossina morsitans Westwood and G. austeni Newstead (Dipt., Glossinidae) in relation to blood feeding.—Bull. ent. Res. 66, 389–399.CrossRefGoogle Scholar
Gillott, C. & Langley, P. A. (1981). The control of receptivity and ovulation in the tsetse fly, Glossina morsitans.—Physiol. Entomol. 6, 269–281.CrossRefGoogle Scholar
Glasgow, J. P. (1963). The distribution and abundance of tsetse.—241 pp. Oxford, Pergamon. (Int. Ser. Monogr. Pure Appl. Biol. Div. Zool. no. 20).Google Scholar
Hargrove, J. W. (1980). The effect of model size and ox odour on the alighting response of Glossina morsitans Westwood and G. pallidipes Austen (Diptera: Glossinidae).—Bull. ent. Res. 70, 229–234.CrossRefGoogle Scholar
Hargrove, J. W. (1988). Tsetse: the limits to population growth.—Med. Vet. Entomol. 2, 203–217.CrossRefGoogle ScholarPubMed
Harley, J. M. B. (1966). Studies on age and trypanosome infection rate in females of Glossina pallidipes Aust., G. palpalis fuscipes Newst. and G. brevipalpis Newst. in Uganda.—Bull. ent. Res. 57, 23–37.CrossRefGoogle Scholar
Harley, J. M. B. & Cockings, K. L. (1968). A colony of G. pallidipes.—Rep. E. Afr. Trypan. Res. Org. 1967, 35–36.Google Scholar
Jaenson, T. G. T. (1978). Reproductive biology of the tsetse Glossina pallidipes Austen (Diptera, Glossinidae) with special reference to mating behaviour.—Ph.D. thesis, Univ. Uppsala, Sweden.Google Scholar
Jaenson, T. G. T. (1980). Mating behaviour of females of Glossina pallidipes Austen (Diptera: Glossinidae).—Bull. ent. Res. 70, 49–60.CrossRefGoogle Scholar
Jaenson, T. G. T. & Takken, W. (1980). Rearing of Glossina pallidipes using membrane-feeding technology.—Entomologia exp. appl. 27, 102–104.CrossRefGoogle Scholar
Jordan, A. M. (1972). The inseminating potential of male Glossina austeni Newst. and G. morsitans morsitans Westw. (Dipt., Glossinidae).—Bull. ent. Res. 62, 319–325.CrossRefGoogle Scholar
Langley, P. A., Maudlin, I. & Leedham, M. P. (1984). Genetic and behavioural differences between Glossina pallidipes from Uganda and Zimbabwe.—Entomologia exp. appl. 35, 55–60.CrossRefGoogle Scholar
Leegwater-Van Der Linden, M. E. (1980). Recent advances in the rearing of Glossina pallidipes Austen.—pp. 413–423 in Isotope and radiation research on animal diseases and their vectors. Proceedings of the International Symposium on the Use of Isotopes for Research and Control of Vectors of Animal Diseases, Host Pathogen Relationships and the Environmental Impact of Control Procedures, jointly organized by the International Atomic Energy Agency and the Food and Agriculture Organization of the United Nations, and held in Vienna, 7–11 05 1979.—468 pp. Vienna, IAEA.Google Scholar
Leegwater-Van Der Linden, M. E. (1981). Effects of a four-day-a-week feeding regimen versus daily feeding on the reproduction of Glossina pallidipes.—Entomologia exp. appl. 29, 169–176.CrossRefGoogle Scholar
Leegwater-Van Der Linden, M. E. & Tiggelman, E. P. M. (1984). Multiple mating and inseminating potential of Glossina pallidipes.—Entomologia exp. appl. 35, 283–294.CrossRefGoogle Scholar
Mews, A. R., Langley, P. A., Pimley, R. W. & Flood, M. E. (1977). Large-scale rearing of tsetse flies (Glossina spp.) in the absence of a living host.—Bull. ent. Res. 67, 119–128.CrossRefGoogle Scholar
Nash, T. A. M., Jordan, A. M. & Trewern, M. A. (1971). Mass rearing of tsetse flies (Glossina spp.): recent advances.—pp. 99–110 in Sterility principle for insect control or eradication. Proceedings of a Symposium on the Sterility Principle for Insect Control or Eradication, jointly organized by the IAEA and FAO and held in Athens, 14 pp. Vienna, IAEA.Google Scholar
Ochieng, R. S., Otieno, L. H. & Banda, H. K. (1987). Performance of the tsetse fly Glossina pallidipes reared under simple laboratory conditions.—Entomologia exp. appl. 45, 265–270.CrossRefGoogle Scholar
Rogers, A. & Kenyanjui, E. N. F. (1972). The maintenance of a colony of Glossina pallidipes Austen.—Ann. trop. Med. Parasit. 66, 267–280.CrossRefGoogle ScholarPubMed
Saunders, D. S. (1962). Age determination for female tsetse flies and the age compositions of samples of Glossina pallidipes Aust., G. palpalis fuscipes Newst. and G. brevipalpis Newst.—Bull. ent. Res. 53, 579–595.CrossRefGoogle Scholar
Saunders, D. S. & Dodd, C. W. H. (1972). Mating, insemination and ovulation in the tsetse fly, Glossina morsitans.—J. Insect. Physiol. 18, 187–198.CrossRefGoogle Scholar
Taylor, P. (1979). The construction of a life-table for Glossina morsitans morsitans Westwood (Diptera: Glossinidae) from seasonal age-measurements of a wild population.—Bull. ent. Res. 69, 553–560.CrossRefGoogle Scholar
Vale, G. A. (1974). The responses of tsetse flies (Diptera, Glossinidae) to mobile and stationary baits.—Bull. ent. Res. 64, 545–588.CrossRefGoogle Scholar
Vale, G. A. & Hargrove, J. W. (1979). A method of studying the efficiency of traps for tsetse flies (Diptera: Glossinidae) and other insects.—Bull. ent. Res. 69, 183–193.CrossRefGoogle Scholar