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Integrating DNA barcodes and morphology for species delimitation in the Corynoneura group (Diptera: Chironomidae: Orthocladiinae)

Published online by Cambridge University Press:  10 October 2013

F.L. Silva  and
S. Wiedenbrug
F.L. Silva*
Affiliation:
Laboratory of Aquatic Entomology, Department of Hydrobiology, Federal University of São Carlos, P.O. Box 676, 13565-905, São Carlos, SP, Brazil
S. Wiedenbrug
Affiliation:
Museum of Zoology, University of São Paulo, Av. Nazaré, 481, 04263-000, São Paulo, SP, Brazil
*
*Author for correspondence E-mail: [email protected]
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Abstract

In this study, we use DNA barcodes for species delimitation to solve taxonomic conflicts in 86 specimens of 14 species belonging to the Corynoneura group (Diptera: Chironomidae: Orthocladiinae), from the Atlantic Forest, Brazil. Molecular analysis of cytochrome c-oxidase subunit I (COI) gene sequences supported 14 cohesive species groups, of which two similar groups were subsequently associated with morphological variation at the pupal stage. Eleven species previously described based on morphological criteria were linked to DNA markers. Furthermore, there is the possibility that there may be cryptic species within the Corynoneura group, since one group of species presented internal grouping, although no morphological divergence was observed. Our results support DNA-barcoding as an excellent tool for species delimitation in groups where taxonomy by means of morphology is difficult or even impossible.

Keywords

DNA-barcodingDNA markersOrthocladiinaeNeotropicalBrazil
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 104 , Issue 1 , February 2014 , pp. 65 - 78
Copyright
Copyright © Cambridge University Press 2013 

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