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Induced resistance against the Asian citrus psyllid, Diaphorina citri, by β-aminobutyric acid in citrus

Published online by Cambridge University Press:  16 April 2013

Siddharth Tiwari ,
Wendy L. Meyer  and
Lukasz L. Stelinski
Siddharth Tiwari
Affiliation:
Entomology and Nematology Department, Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, USA
Wendy L. Meyer
Affiliation:
Entomology and Nematology Department, Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, USA
Lukasz L. Stelinski*
Affiliation:
Entomology and Nematology Department, Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, USA
*
*Author for correspondence Phone: +1 863 956 8851 Fax: +1 863 956 4631 E-mail: [email protected]
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Abstract

β-Aminobutyric acid (BABA) is known to induce resistance to microbial pathogens, nematodes and insects in several host plant/pest systems. The present study was undertaken to determine whether a similar effect of BABA occurred against the Asian citrus psyllid, Diaphorina citri Kuwayama, in citrus. A 25 mM drench application of BABA significantly reduced the number of eggs/plant as compared with a water control, whereas 200 and 100 mM applications of BABA reduced the numbers of nymphs/plant and adults/plants, respectively. A 5 mM foliar application of BABA significantly reduced the number of adults but not eggs or nymphs when compared with a water control treatment. In addition, leaf-dip bioassays using various concentrations (25–500 mM) of BABA indicated no direct toxic effect on 2nd and 5th instar nymphs or adult D. citri. BABA-treated plants were characterized by significantly lower levels of iron, magnesium, phosphorus, sodium, sulfur and zinc as compared with control plants. The expression level of the PR-2 gene (β-1,3-glucanase) in BABA-treated plants that were also damaged by D. citri adult feeding was significantly higher than in plants exposed to BABA, D. citri feeding alone or control plants. Our results indicate the potential for using BABA as a systemic acquired resistance management tool for D. citri.

Keywords

BABAcitrus greeninggene expressionimidaclopridinduced resistancepathogenesis-related proteinsPR-2 genesystemic acquired resistance
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 103 , Issue 5 , October 2013 , pp. 592 - 600
Copyright
Copyright © Cambridge University Press 2013 

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