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Incidence of insecticide resistance alleles in sexually-reproducing populations of the peach–potato aphid Myzus persicae (Hemiptera: Aphididae) from southern France

Published online by Cambridge University Press:  09 March 2007

T. Guillemaud ,
A. Brun ,
N. Anthony ,
M.H. Sauge ,
R. Boll ,
R. Delorme ,
D. Fournier ,
L. Lapchin  and
F. Vanlerberghe-Masutti
T. Guillemaud*
Affiliation:
Equipe ‘Ecotoxicologie et Résistance aux Insecticides’, UMR 1112 INRA, 123 Boulevard du Cap, 06606 Antibes, France Equipe ‘Biologie et Gestion des Populations d'insectes’, UMR 1112 INRA, 06606 Antibes, France
A. Brun
Affiliation:
Equipe ‘Ecotoxicologie et Résistance aux Insecticides’, UMR 1112 INRA, 123 Boulevard du Cap, 06606 Antibes, France
N. Anthony
Affiliation:
The Biodiversity and Ecological Processes Research Group, School of Biosciences, Cardiff University, PO Box 915, Cardiff, CF10 3TL, UK
M.H. Sauge
Affiliation:
Equipe ‘Résistance des Plantes aux Insectes’, UMR INRA/UAPV Ecologie des Invertébrés, 84914 Avignon, France
R. Boll
Affiliation:
Equipe ‘Biologie et Gestion des Populations d'insectes’, UMR 1112 INRA, 06606 Antibes, France
R. Delorme
Affiliation:
Unité de Phytopharmacie et Médiateurs Chimiques INRA, Route de Saint-Cyr, 78026 Versailles Cedex, France
D. Fournier
Affiliation:
Groupe de Biochimie des Protéines, bat 4R3B1, LSPCMIB, Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse, France
L. Lapchin
Affiliation:
Equipe ‘Biologie et Gestion des Populations d'insectes’, UMR 1112 INRA, 06606 Antibes, France
F. Vanlerberghe-Masutti
Affiliation:
Equipe ‘Ecotoxicologie et Résistance aux Insecticides’, UMR 1112 INRA, 123 Boulevard du Cap, 06606 Antibes, France Equipe ‘Biologie et Gestion des Populations d'insectes’, UMR 1112 INRA, 06606 Antibes, France
*
*Fax: (33) 493 67 89 55 E-mail: [email protected]
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Abstract

Intensive chemical treatments have led to the development of a number of insecticide resistance mechanisms in the peach–potato aphid Myzus persicae (Sulzer). Some of these mechanisms are known to be associated with negative pleiotropic effects (resistance costs). Molecular and biochemical methods were used to determine the genotypes or phenotypes associated with four insecticide resistance mechanisms in single aphids from sexually-reproducing populations in southern France. The mechanisms considered were E4 and FE4 carboxylesterase overproduction, modified acetycholinesterase, and kdr and rdl resistance-associated mutations. A new method for determining individual kdr genotypes is presented. Almost all resistant individuals overproduced FE4 carboxylesterase, whereas modified acetylcholinesterase was rare. Both the kdr and rdl resistance mutations were present at high frequencies in French sexually-reproducing populations. The frequencies of insecticide resistance genes were compared before and after sexual reproduction in one peach orchard at Avignon to evaluate the potential impact of selection on the persistence of resistance alleles in the over-wintering phase. The frequencies of the kdr and rdl mutations varied significantly between autumn and spring sampling periods. The frequency of the kdr mutation increased, probably due to pyrethroid treatments at the end of the winter. Conversely, the frequency of the rdl mutation decreased significantly during winter, probably because of a fitness cost associated with this mutation.

Type
Review Article
Information
Bulletin of Entomological Research , Volume 93 , Issue 4 , July 2003 , pp. 289 - 297
Copyright
Copyright © Cambridge University Press 2003

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