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In vitro rearing of Glossina palpalis palpalis (Robineau-Desvoidy) (Diptera: Glossinidae) on guinea-pig blood
Published online by Cambridge University Press: 10 July 2009
Abstract
The productivity of Glossina palpalis palpalis (R.-D.) reared in vitro via silicone rubber membranes on fresh guinea-pig blood equalled the productivity of flies fed in vivo on guinea-pigs. Over 50 days, the number of puparia per initial female was 3·0 in both cases. Puparial weights were greater than 31 mg throughout the four reproductive cycles. While fresh blood was an excellent diet for G. p. palpalis, freeze-dried guinea-pig blood was unsuitable; survival, reproduction and puparial weights were all low for flies fed on freeze-dried blood. The evidence lends support to the theory that there is no inherent nutritional deficiency caused by feeding on blood through a membrane.
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