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Identification and phenology of Hyalesthes obsoletus (Hemiptera: Auchenorrhyncha: Cixiidae) nymphal instars

Published online by Cambridge University Press:  24 February 2012

E. Cargnus ,
F. Pavan ,
N. Mori  and
M. Martini
E. Cargnus
Affiliation:
Department of Agricultural and Environmental Sciences, University of Udine, Via delle Scienze 206, 33100 Udine, Italy
F. Pavan*
Affiliation:
Department of Agricultural and Environmental Sciences, University of Udine, Via delle Scienze 206, 33100 Udine, Italy
N. Mori
Affiliation:
Department of Environmental Agronomy and Crop Sciences, University of Padua, Viale dell'Università 16, 35020 Legnaro (PD), Italy
M. Martini
Affiliation:
Department of Agricultural and Environmental Sciences, University of Udine, Via delle Scienze 206, 33100 Udine, Italy
*
*Author for correspondence Fax: 39 0432 558501 E-mail: [email protected]
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Abstract

Urtica dioica and Convolvulus arvensis are the main host plants of Hyalesthes obsoletus and play an important role in the epidemiology of Bois noir of grapevines. The earliest survey, which was carried out to compare the phenology of nymphal instars on U. dioica and C. arvensis, had highlighted some problems in the identification of the instars. Therefore, the correct identification of nymphs to species and instar level became a preliminary aim of this research. Adults and nymphs attributable to H. obsoletus were collected during 2008–2010 in three flatland vineyard habitats of northern Italy on U. dioica, C. arvensis and Artemisia verlotorum. Nymphs and morphologically identified adults of H. obsoletus were submitted to molecular identification. Morphometric and morphological studies were carried out on nymphs collected in the field or obtained in laboratory rearings. Molecular methods not only confirmed the identity of adults, but also allowed the assignment of the nymphs to this species. Morphometric and morphological characteristics (e.g. body and head-thoracic lengths, number of thoracic pits) showed the existence of five nymphal instars. Morphometric differences between newly hatched and older first-instar nymphs were observed. A key to distinguish the five instars was proposed. Evident differences between H. obsoletus nymphs studied here and elsewhere were identified. According to differences in adult-flight period, an earlier phenology of nymphs on C. arvensis than on U. dioica was observed. In particular, the typical overwintering instar was the second on U. dioica and the third on C. arvensis.

Keywords

Hyalesthes obsoletusnymphsmorphologymolecular identificationphenologyhost plantBois noir
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 102 , Issue 5 , October 2012 , pp. 504 - 514
Copyright
Copyright © Cambridge University Press 2012

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References

Alma, A., Arnò, C., Arzone, A. & Vidano, C. (1988) New biological reports on Auchenorrhyncha in vineyards. pp. 509–516in Vidano, C. & Arzone, A. (Eds) Proceedings of 6th Auchenorrhyncha Meeting. Turin, Italy, 7–11 September 1987.Google Scholar
Alma, A., Soldi, G., Tedeschi, R. & Marzachì, C. (2002) Ruolo di Hyalesthes obsoletus Signoret (Homoptera, Cixiidae) nella trasmissione del legno nero della vite in Italia. Petria 12, 411–412.Google Scholar
Bertin, S., Picciau, L., Àcs, Z., Alma, A. & Bosco, D. (2010a) Molecular differentiation of four Reptalus species (Hemiptera: Cixiidae). Bulletin of Entomological Research 100, 551–558.CrossRefGoogle ScholarPubMed
Bertin, S., Picciau, L., Àcs, Z., Alma, A. & Bosco, D. (2010b) Molecular identification of the Hyalesthes species (Hemiptera: Cixiidae) occurring in vineyard agroecosystems. Annals of Applied Biology 157, 435–445.CrossRefGoogle Scholar
Botti, S., Paltrinieri, S., Mori, N., Milanesi, L., Bondavalli, R. & Bertaccini, A. (2005) Variabilità molecolare di citoplasmi 16SrXII in vigneti delle province di Modena e Reggio Emilia. Petria 15, 121–124.Google Scholar
Bourgoin, T. & Deiss, V. (1994) Sensory plate organs of the antenna in the Meenoplidae-Kinnaridae group (Hemiptera: Fulgoromorpha). International Journal of Insect Morphology and Embryology 23, 159–168.CrossRefGoogle Scholar
Brčak, J. (1979) Leafhopper and planthopper vectors of plant disease agents in central and southern Europe. pp. 97–146in Maramorosch, K. & Harris, K.F. (Eds) Leafhopper Vectors and Plant Disease Agents. London, UK, Academic Press.CrossRefGoogle Scholar
Bressan, A., Turata, R., Maixner, M., Spiazzi, S., Boudon-Padieu, E. & Girolami, V. (2007) Vector activity of Hyalesthes obsoletus living on nettles and transmitting a stolbur phytoplasma to grapevines: a case study. Annals of Applied Biology 150, 331–339.CrossRefGoogle Scholar
Chen, S. & Yang, C.T. (1995) The metatarsi of the Fulgoroidea (Homoptera: Auchenorrhyncha). Chinese Journal of Entomology 15, 257–269.Google Scholar
Cumber, R.A. (1952) Studies on Oliarus atkinsoni Myers (Hem. Cixiidae), vector of the “yellow-leaf” desease of Phornium tenax Forst. II.: The nymphal instars and seasonal changes in the composition of nymphal populations. New Zealand Journal of Science Technology 34, 160–165.Google Scholar
Daly, H.W. (1985) Insect morphometrics. Annual Review of Entomology 30, 415–438.CrossRefGoogle Scholar
Dyar, H.G. (1890) The number of molts of lepidopterous larvae. Psyche 5 (175–176), 420–422.CrossRefGoogle Scholar
Forte, V., Angelini, E., Maixner, M. & Borgo, M. (2010) Preliminary results on population dynamics and host plants of Hyalesthes obsoletus in North-Eastern Italy. Vitis 49, 39–42.Google Scholar
Güclü, S. & Ozbek, H. (1988) Some studies on the biology of Hyalesthes obsoletus Signoret (Homoptera: Cixiidae) in the conditions of Erzurum. Turkiye Entomoloji Dergisi 12, 103–111 (in Turkish).Google Scholar
Holzinger, W.E., Kammerlander, I. & Nickel, H. (2003) The Auchenorrhyncha of Central Europe – Die Zikaden Mitteleuropas Volume 1: Fulgoromoropha, Cicadomorpha excl. Cicadellidae. Leiden, The Netherlands, Brill.CrossRefGoogle Scholar
Kessler, S. & Kehrli, P. (2009) Host plant preferences of Hyalesthes obsoletus in vineyards of Western Switzerland. pp. 50–54in Kast, W.K., Stark-Urnau, M. & Bleyer, K. (Eds) Proeedings 1st European Bois noir Workshop. Weinsberg, Germany, 11–13 November 2008.Google Scholar
Kessler, S., Schaerer, S., Delabays, N., Turlings, T.C.J., Trivellone, V. & Kehrli, P. (2011) Host plant preferences of Hyalesthes obsoletus, the vector of the grapevine yellows disease ‘bois noir’, in Switzerland. Entomologia Experimentalis et Applicata 139, 60–67.CrossRefGoogle Scholar
Johannesen, J., Lux, B., Michel, K., Seitz, A. & Maixner, M. (2008) Invasion biology and host specificity of the grapevine yellows disease vector Hyalesthes obsoletus in Europe. Entomologia Experimentalis et Applicata 126, 217–227.CrossRefGoogle Scholar
Langer, M., Darimont, H. & Maixner, M. (2003) Control of phytoplasma vectors in organic viticulture. IOBC/wprs Bulletin 26(8), 197–202.Google Scholar
Lessio, F., Tedeschi, R. & Alma, A. (2007) Population dynamics, host plants and infection rate with stolbur phytoplasma of Hyalesthes obsoletus Signoret in north-western Italy. Journal of Plant Pathology 89, 97–102.Google Scholar
Maixner, M. (1994) Transmission of German grapevine yellows (Vergilbungskrankheit) by the planthopper Hyalesthes obsoletus (Auchenorrhyncha: Cixiidae). Vitis 33, 103–104.Google Scholar
Maixner, M. (2007) Biology of Hyalesthes obsoletus and approaches to control this soilborne vector of Bois noir disease. IOBC/wprs Bulletin 30(7), 3–9.Google Scholar
Maixner, M. (2010) Phytoplasma epidemiological systems with multiple plant hosts. pp. 213–232in Weintraub, P.G. & Jones, P.G. (Eds) Phytoplasmas: Genomens, Plant Hosts and Vectors. Wallingford, UK, CABI Publishing.Google Scholar
Maixner, M. & Langer, M. (2006) Prediction of the flight of Hyalesthes obsoletus, vector of stolbur phytoplasma, using temperature sums. IOBC/wprs Bulletin 29(11), 161–166.Google Scholar
Mori, N., Pavan, F., Bacchiavini, M., Reggiani, N., Bonomi, F. & Bertaccini, A. (2008a) Fenologia di Hyalesthes obsoletus Signoret su convolvolo e ortica. Petria 18, 229–231.Google Scholar
Mori, N., Pavan, F., Bondavalli, R., Reggiani, N., Paltrinieri, S. & Bertaccini, A. (2008b) Factors affecting the spread of “Bois Noir” disease in north Italy vineyards. Vitis 47, 65– 72.Google Scholar
Musil, M. (1956) Příspěvek k poznání vzrůstových stupňů žilnatky vironosné (Hyalesthes obsoletus Sign.). Zoologické Listy 19, 17–22.Google Scholar
Myers, J.G. (1929) Observations on the biology of two remarkable cixiid plant-hoppers (Homoptera) from Cuba. Psyche 36, 283–292.CrossRefGoogle Scholar
Palermo, S., Elekes, M., Botti, S., Ember, I., Alma, A., Orosz, A., Bertaccini, A. & Kölber, M. (2004) Presence of stolbur phytoplasma in Cixiidae in Hungarian vineyards. Vitis 43, 201–203.Google Scholar
Patwary, M.U., Kenchington, E.L., Bird, C.J. & Zouros, E. (1994) The use of random amplified polymorphic DNA (RAPD) markers in genetic studies of the sea scallop Placopecten magellanicus (Gmelin, 1791). Journal of Shellfish Research 13, 547–553.Google Scholar
Riedle-Bauer, M., Tiefenbrunner, W., Otreba, J., Hanak, K., Schildberger, B. & Regner, F. (2006) Epidemiological observations on Bois Noir in Austrian vineyards. Mitteilungen Klosterneuburg, Rebe und Wein, Obstbau und Früchteverwertung 56, 177–181.Google Scholar
Simon, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H. & Flook, P. (1994) Evolution, weighting and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Annals of the Entomological Society of America 87, 651–701.CrossRefGoogle Scholar
Sforza, R., Bourgoin, T., Wilson, S.W. & Boudon-Padieu, E. (1999) Field observation, laboratory rearing and description of immatures of the planthopper Hyalesthes obsoletus (Hemiptera: Cixiidae). European Journal of Entomology 96, 409–418.Google Scholar
Shih, H.T. & Yang, C.T. (1996) The antennal second projection of Cixiidae (Homoptera: Fulgoroidea). Chinese Journal of Entomology 16, 279–285.Google Scholar
Trivellone, V., Pinzauti, F. & Bagnoli, B. (2005) Reptalus quinquecostatus (Dufour) (Auchenorrhyncha Cixiidae) as a possible vector of Stolbur-phytoplasma in a vineyard in Tuscany. Redia 88, 103–108.Google Scholar
Wilson, S.W. & Tsai, J.H. (1982) Descriptions of the immature stages of Myndus crudus (Homoptera: Fulgoroidea: Cixiidae). Journal of the New York Entomological Society 90, 166–175.Google Scholar
Wilson, S.W., Tsai, J.H. & Thompson, C.R. (1983) Description of the nymphal instars of Oecleus borealis (Homoptera: Fulgoroidea: Cixiidae). Journal of the New York Entomological Society 91, 418–423.Google Scholar