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Genetic variation of Marchalina hellenica (Hemiptera: Margarodidae) sampled from different hosts and localities in Greece

Published online by Cambridge University Press:  09 March 2007

J.T. Margaritopoulos ,
N. Bacandritsos ,
A.N. Pekas ,
C. Stamatis ,
Z. Mamuris  and
J.A. Tsitsipis
J.T. Margaritopoulos*
Affiliation:
Department of Agriculture, Crop Production and Agricultural Environment, University of Thessaly, Fytokou Str.,38446 Nea Ionia, Magnesia, Greece
N. Bacandritsos
Affiliation:
Institute of Veterinary Research of Athens, Neapoleos 25, Agia Paraskevi, 15310 Athens, Greece
A.N. Pekas
Affiliation:
Department of Agriculture, Crop Production and Agricultural Environment, University of Thessaly, Fytokou Str.,38446 Nea Ionia, Magnesia, Greece
C. Stamatis
Affiliation:
Department of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26, Larissa 41221, Greece
Z. Mamuris
Affiliation:
Department of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26, Larissa 41221, Greece
J.A. Tsitsipis
Affiliation:
Department of Agriculture, Crop Production and Agricultural Environment, University of Thessaly, Fytokou Str.,38446 Nea Ionia, Magnesia, Greece
*
*Fax: +30 24210 93286 E-mail: [email protected]
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Abstract

Random amplified polymorphic DNA (RAPD) analysis was applied to 120 individuals of Marchalina hellenica (Gennadius) representing six populations collected in northern, central and southern mainland Greece. One population was sampled on one species of fir tree and the others on two species of pine trees. Four random decamer primers were used to evaluate genetic variation among the populations examined. The results revealed intra- and interpopulation polymorphism both related to host type and region of origin. Phylogenetic analysis based on genetic distances estimated by the RAPD frequencies revealed an important genetic differentiation in samples collected on fir trees in southern Greece and to a lesser extent in samples from pine trees in central and northern Greece. Furthermore, considerable subdivision and restricted gene flow among the populations examined were observed. The results are discussed in relation to the biology and geographical distribution of M. hellenica in Greece.

Type
Research Article
Information
Bulletin of Entomological Research , Volume 93 , Issue 5 , September 2003 , pp. 447 - 453
Copyright
Copyright © Cambridge University Press 2003

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