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Functional analysis of a NF-κB transcription factor in the immune defense of Oriental fruit fly, Bactrocera dorsalis Hendel (Diptera: Tephritidae)

Published online by Cambridge University Press:  22 November 2016

Z. Shi ,
H. Liang  and
Y. Hou
Z. Shi
Affiliation:
State Key Laboratory of Ecological Pest Control of Fujian-Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, 350002, China Fujian Provincial Key Laboratory of Insect Ecology, College of Plant Protection, Fujian Agriculture & Forestry University (FAFU), Fuzhou, China
H. Liang
Affiliation:
State Key Laboratory of Ecological Pest Control of Fujian-Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, 350002, China Fujian Provincial Key Laboratory of Insect Ecology, College of Plant Protection, Fujian Agriculture & Forestry University (FAFU), Fuzhou, China
Y. Hou*
Affiliation:
State Key Laboratory of Ecological Pest Control of Fujian-Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, 350002, China Fujian Provincial Key Laboratory of Insect Ecology, College of Plant Protection, Fujian Agriculture & Forestry University (FAFU), Fuzhou, China
*
*Author for correspondence Phone: +86-591-83768654 Fax: +86-591-83768654 E-mail: [email protected]
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Abstract

Although some novel antimicrobial peptides (AMP) have been successfully isolated from Bactrocera dorsalis Hendel, the mechanisms underlying the induction of these peptides are still elusive. The homolog of NF-κB transcription factor Relish, designated as BdRelish, was cloned from B. dorsalis. The full length cDNA of BdRelish is 3954 bp with an open reading frame that encodes 1013 amino acids. Similar to Drosophila Relish and the mammalian p100, it is a compound protein containing a conserved Rel homology domain, an IPT (Ig-like, plexins, transcription factors) domain and an IκB-like domain (four ankyrin repeats), the nuclear localization signal RKRRR is also detected at the residues 449–453, suggesting that it has homology to Relish and it is a member of the Rel family of transcription activator proteins. Reverse transcription quantitative polymerase chain reaction analysis reveals that BdRelish mRNAs are detected in different quantities from various tissues and the highest transcription level of BdRelish is determined in fat body. The injection challenge of Escherichia coli and Staphylococcus aureas significantly upregulated the expression of BdRelish. The injection of BdRelish dsRNA markedly reduced the expression of BdRelish and decreased the transcription magnitude of antimicrobial peptides. Individuals injected BdRelish dsRNA died at a significantly faster rate compared with the control groups. Therefore, BdRelish is vital for the transcription of AMPs to attack the invading bacteria.

Keywords

insect immunityRelishantimicrobial peptideBactrocera dorsalis
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 107 , Issue 2 , April 2017 , pp. 251 - 260
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Copyright
Copyright © Cambridge University Press 2016 

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