Hostname: page-component-cd9895bd7-8ctnn Total loading time: 0 Render date: 2024-12-31T17:11:07.407Z Has data issue: false hasContentIssue false
  • English
  • Français

F2 screen for resistance to a Bacillus thuringiensis-maize hybrid in the sugarcane borer (Lepidoptera: Crambidae)

Published online by Cambridge University Press:  04 October 2007

F.N. Huang ,
B.R. Leonard  and
D.A. Andow
F.N. Huang*
Affiliation:
Department of Entomology, 404 Life Sciences Building, Louisiana State University AgCenter, Baton Rouge, Louisiana70803, USA
B.R. Leonard
Affiliation:
Macon Ridge Research Station, Louisiana State University AgCenter, WinnsboroLA 71295, USA
D.A. Andow
Affiliation:
Department of Entomology, University of Minnesota, St. Paul, MN55108, USA
*
*Fax: +1225 578 1634 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

A novel F2 screening technique was developed for detecting resistance in sugarcane borer, Diatraea saccharalis (F.), to transgenic Bacillus thuringiensis (Bt)-maize expressing the Cry1Ab insecticidal protein. The F2 screening method involved (i) collecting larvae from maize fields; (ii) establishing two-parent families; (iii) screening F2 neonates for survival on Bt-maize leaf tissues; and (iv) confirming resistance on commercial Bt-maize plants. With the F2 screening method, 213 iso-line families of D. saccharalis were established from field collections in northeast Louisiana, USA and were screened for Bt resistance. One family was confirmed to carry a major Bt resistance allele(s). In a laboratory bioassay, larval mortality of the Bt-resistant D. saccharalis on Bt-maize leaf tissues was significantly lower than that of a Bt-susceptible strain. This Bt-resistant D. saccharalis population is the first corn stalk borer species that has completed larval development on commercial Bt-maize. The F2 screening protocol developed in this study could be modified for detecting Bt resistance alleles in other similar corn stalk borers, such as the European corn borer, Ostrinia nubilalis (Hübner), and the southwestern corn borer, D. grandiosella Dyar.

Keywords

Diatraea saccharalisBt-maizeresistance monitoringinsecticide resistance management
Type
Research Article
Information
Bulletin of Entomological Research , Volume 97 , Issue 5 , October 2007 , pp. 437 - 444
Copyright
Copyright © Cambridge University Press 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abbott, W.S. (1925) A method for computing the effectiveness of an insecticide. Journal of Economic Entomology 18, 265–267.CrossRefGoogle Scholar
Andow, D.A. & Alstad, D.N. (1998) F2 screen for rare resistance alleles. Journal of Economic Entomology 91, 572–578.CrossRefGoogle Scholar
Andow, D.A., Alstad, D.N., Pang, Y.H., Bolin, P.C. & Hutchison, W.D. (1998) Using an F2 screen to search for resistance alleles to Bacillus thuringiensis toxin in European corn borer (Lepidoptera: Crambidae). Journal of Economic Entomology 91, 579–584.CrossRefGoogle Scholar
Andow, D.A., Olson, D.M., Hellmich, R.L., Alstad, D.N. & Hutchison, W.D. (2000) Frequency of resistance to Bacillus thuringiensis toxin Cry1Ab in an Iowa population of European corn borer (Lepidoptera: Crambidae). Journal of Economic Entomology 93, 26–30.Google Scholar
Bentur, J.S., Andow, D.A., Cohen, M.B., Romena, A.M. & Gould, F. (2000) Frequency of alleles conferring resistance to a Bacillus thuringiensis toxin in a Philippine population of Scirpophaga incertulas (Lepidoptera: Pyralidae). Journal Economic Entomology 93, 1515–1521.CrossRefGoogle Scholar
Bolin, P.C., Hutchison, W.D., Andow, D.A. & Ostlie, K.R. (1998) Monitoring for European corn borer (Lepidoptera: Crambidae) resistance to Bacillus thuringiensis: logistical considerations when sampling larvae. Journal of Agricultural Entomology 15, 231–238.Google Scholar
Bourguet, D., Chaufaux, J., Séguin, M., Buisson, C., Hinton, J.L., Stodola, T.J., Porter, P., Cronholm, G., Buschman, L.L. & Andow, D.A. (2003) Frequency of alleles conferring resistance to Bt maize in French and US corn belt popu-lations of the European corn borer, Ostrinia nubilalis. Theoretical and Applied Genetics 106, 1225–1233.CrossRefGoogle Scholar
Burd, A.D., Gould, F., Bradley, J.R., Vanduyn, J.W. & Moar, W.J. (2003) Estimated frequency of non-recessive Bt resistance genes in bollworm, Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) in eastern North Carolina. Journal Economic Entomology 96, 137–142.CrossRefGoogle Scholar
Butt, B.A. & Cantu, E. (1962) Sex determination of Lepidopterous pupae. USDA, Agricultural Research Service Report 33–75, 1–7.Google Scholar
Castro, B.A., Riley, T.J., Leonard, B.R. & Baldwin, J. (2004) Borers galore: Emerging pest in Louisiana corn, grain sorghum and rice. Louisiana Agriculture 47, 4–6.Google Scholar
Cochran, W.G.& Cox, G.M. (1950) Experimental Designs. 451 pp. New York, John Wiley & Sons, Inc.Google Scholar
Farinós, G.P., Poza, M., Hernández-Crespo, P., Ortego, F. & Castañera, P. (2004) Resistance monitoring of field populations of the corn borers Sesamia nonagrioides and Ostrinia nubilalis after 5 years of Bt maize cultivation in Spain. Entomologia Experimentalis et Applicata 110, 23–30.Google Scholar
Génissel, A., Augustin, S., Courtin, C., Pilate, G., Lorme, P. & Bourguet, D. (2003) Initial frequency of alleles conferring resistance to Bacillus thuringiensis poplar in a field population of Chrysomela tremulae. Proceedings of the Royal Society of London. Series B, Biological Sciences 270, 791–797.CrossRefGoogle Scholar
Gould, F. (1998) Sustainability of transgenic insecticidal cultivars: integrating pest genetics and ecology. Annual Review of Entomology 43, 701–726.CrossRefGoogle ScholarPubMed
Huang, F. (2006) Detection and monitoring of insect resistance to Bt crops. Insect Science 13, 73–90.Google Scholar
Huang, F., Leonard, B.R. & Gable, R.H. (2006) Comparative susceptibility of European corn borer, southwestern corn borer, and sugarcane borer (Lepidoptera: Crambidae) to Cry1ab protein in a commercial Bt-corn hybrid. Journal of Economic Entomology 99, 194–202.Google Scholar
Huang, F., Leonard, B.R., Cook, D.R., Lee, D.R., Andow, D.A., Baldwin, J.L., Tindall, K.V. & Wu, X. (2007) Frequency of alleles conferring resistance to Bacillus thuringiensis maize in Louisiana populations of southwestern corn (Lepidoptera: Crambidae). Entomologia Experimentalis et Applicata 122, 53–58.CrossRefGoogle Scholar
James, C. (2004) Global status of commercialized biotech/GM crops: 2004. ISAAA Briefs (Ithaca, NY, ISAAA) 32, 1–12.Google Scholar
Li, G., Wu, K., Gould, F., Feng, H., He, Y. & Guo, Y. (2004) Frequency of Bt resistance genes in Hellicoverpa armigera populations from the yellow river cotton-farming region of China. Entomologia Expermentale Applicta 112, 135–143.Google Scholar
Marçon, P.C.R.G., Young, L.J., Steffey, K.L. & Siegfried, B.D. (1999) Baseline susceptibility of European corn borer (Lepidoptera: Crambidae) to Bacillus thuringiensis toxins. Journal Economic Entomology 92, 279–285.Google Scholar
Marçon, P.C.R.G., Siegfried, B.D., Spencer, T. & Hutchison, W.D. (2000) Development of diagnostic concentrations for monitoring Bacillus thuringiensis resistance in European corn borer (Lepidoptera: Crambidae). Journal of Economic Entomology 93, 925–930.CrossRefGoogle ScholarPubMed
NASS (National Agricultural Statistics Service) (2005) Acreage. USDA, Washington, DC. http://usda.mannlib.cornell.edu/reports/nassr/field/pcp-bba/acrg0605.pdfGoogle Scholar
National Research Council (2002) Environmental Effects of Transgenic Plants: The Scope and Adequacy of Regulation. 320 pp. Washington, DC, National Academy.Google Scholar
Ostlie, K.R., Hutchison, W.D. & Hellmich, R.L. (1997) Bt-corn & European corn borer, Long term success through resistance management. North Central Region Extension Publication (St. Paul, MN, University of Minnesota) 602, 18.Google Scholar
Plapp, F.W. Jr, Campanhola, C., Bagwell, R.D. & McCutcheon, B.F. (1990) Management of pyrethroid-resistant tobacco budworms on cotton in the United States. pp. 237–261in Roush, R.T. & Tabashnik, B.E. (Eds) Pesticide Resistance in Arthropods. New York, NJ, Chapman & Hall.Google Scholar
Reed, J.P. & Halliday, W.R. (2001) Establishment of Cry9C susceptibility baselines for European corn borer and southwestern corn borer (Lepidoptera: Crambidae). Journal of Economic Entomology 94, 397–402.CrossRefGoogle ScholarPubMed
Ritchie, S.W., Hanway, J.J., Benson, G.O. & Herman, J.C. (1993) How a corn plant develops. Iowa State University Cooperative Extension Service. Special Report 48. http://maize.agron.iastate.edu/corngrows.html.Google Scholar
Roush, R.T. & Miller, G.L. (1986) Considerations for design of insecticide resistance monitoring program. Journal of Economic Entomology 79, 293–298.CrossRefGoogle Scholar
SAS Institute (1999) SAS/STAT User Guide, Version 8. Cary, NC, SAS Institute.Google Scholar
Stodola, T.J. & Andow, D.A. (2004) F2 screen variations and associated statistics. Journal of Economic Entomology 97, 1756–1764.Google Scholar
Stodola, T.J., Andow, D.A., Hyden, A.R., Hinton, J.L., Roark, J.J., Buschman, L.L., Porter, P. & Cronholm, G.B. (2006) Frequency of resistance to Bacillus thuringiensis toxin Cry1Ab in Southern US corn belt population of European corn borer (Lepidoptera: Crambidae). Journal of Economic Entomology 99, 502–507.CrossRefGoogle Scholar
Tabashnik, B.E., Patin, A.L., Dennehy, T.J., Liu, Y.B., Carrière, Y., Sims, M.A. & Antilla, L. (2000) Frequency of resistance to Bacillus thuringiensis in field populations of pink bollworm. Proceedings of National Academy Sciences, USA 97, 12980–12984.CrossRefGoogle ScholarPubMed
Tabashnik, B.E., Carriere, Y., Dennehy, T.J., Morin, S., Sisterson, M.S., Roush, R.T., Shelton, A.M. & Zhao, J.Z. (2003) Insect resistance to transgenic Bt crops: lessons from the laboratory and field. Journal of Economic Entomology 96, 1031–1038.Google Scholar
Trisyono, Y.A. & Chippendale, G.M. (2002) Susceptibility of field-collected populations of the southwestern corn borer, Diatraea grandiosella, to Bacillus thuringiensis. Pest Management Science 58, 1022–1028.Google Scholar
Wu, K., Guo, Y., Lv, N., Greenplate, J.T. & Deaton, R. (2002) Resistance monitoring of Helicoverpa armigera (Lepidoptera: Noctuide) to Bacillus thuringiensis insecticidal protein in China. Journal of Economic Entomology 95, 826–831.CrossRefGoogle Scholar
Zar, J.H. (1984) Biostatistical Analysis. 2nd edn. 718 pp. Englewood Cliffs, NJ, Prentice Hall.Google Scholar
Zhao, J.Z., Li, Y.H., Collins, H.L. & Shelton, A.M. (2002) Examination of the F2 screen for rare resistance alleles to Bacillus thuringiensis toxins in the diamondback moth (Lepidoptera: Plutellidae). Journal of Economic Entomology 95, 14–21.CrossRefGoogle ScholarPubMed