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Evaluation of resistance, cross-resistance and synergism of abamectin and teflubenzuron in a multi-resistant field population of Plutella xylostella (Lepidoptera: Plutellidae)

Published online by Cambridge University Press:  10 July 2009

M. Iqbal  and
D.J. Wright
M. Iqbal
Affiliation:
Department of Biology, Imperial College at Silwood Park, Ascot, Berkshire, SL5 7PY, UK
D.J. Wright*
Affiliation:
Department of Biology, Imperial College at Silwood Park, Ascot, Berkshire, SL5 7PY, UK
*
* Author for correspondence.
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Abstract

The efficacy of abamectin (AgrimecR) and teflubenzuron (NomoltR) was assessed by leaf-dip bioassay against larvae of the diamondback moth, Plutella xylostella Linnaeus from a population (SERD3) collected originally in lowland Malaysia in December 1994. Evidence for resistance to both abamectin and teflubenzuron was found in the F7 generation (LC50 ratio of 60 and 24 respectively compared with a laboratory, insecticide-susceptible strain). Selection of sub-populations of SERD3 (F7–F9) with abamectin and teflubenzuron increased the LC50 ratio to 220 and 360 respectively and estimates of realized heritability [h2] were high (c. 0.8 and 0.9) for both compounds. There was no cross-resistance between these compounds in the abamectin and teflubenzuron-selected sub-populations but some indication of negatively-correlated resistance. Topical application of the synergists piperonyl butoxide, S,S,S-tributylphosphorotrithioate and maleic acid diethyl ester to the laboratory strain had no significant effect on the toxicity of abamectin or teflubenzuron in subsequent leaf-dip assays. In contrast, pre-treatment with piperonyl butoxide and S,S,S-tributylphosphorotrithioate significantly increased the toxicity of abamectin (c. 4- and 3-fold) and teflubenzuron (c. 7- and 19-fold) in the abamectin and teflubenzuron-selected sub-populations of SERD3, suggesting that microsomal monoxygenases and/or esterases may be involved in resistance. Pre-treatment with maleic acid diethyl ester only increased the toxicity of abamectin by c. 2-fold and had no significant effect on the toxicity of teflubenzuron, providing limited evidence for the involvement of glutathione-S-transferases in resistance to the former compound alone.

Type
Research Article
Information
Bulletin of Entomological Research , Volume 87 , Issue 5 , October 1997 , pp. 481 - 486
Copyright
Copyright © Cambridge University Press 1997

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References

Abbott, W.S. (1925) A method for computing the effectiveness of an insecticide. Journal of Economic Entomology 18, 265–267.CrossRefGoogle Scholar
Aitken, M., Anderson, B., Francis, B. & Hinde, J. (1989) Statistical modelling in GLIM. Oxford, Clarendon Press.Google Scholar
Apperson, C.S. & Georghiou, G.P. (1975) Mechanisms of resistance to organophosphorus insecticides in Culex tarsalis. Journal of Economic Entomology 68, 153–157.CrossRefGoogle ScholarPubMed
Casida, J.E. (1970) Mixed-function oxidase involvement in the biochemistry of insecticide synergists. Journal of Agriculture and Food Chemistry 18, 753–772.CrossRefGoogle ScholarPubMed
Clark, J.M., Scott, J.G., Campos, F. & Bloomquist, J.R. (1995) Resistance to avermectins – extent, mechanisms and management implications. Annual Reviews in Entomology 40, 1–30.CrossRefGoogle ScholarPubMed
Croft, B.A. (1990) Arthropod biological control agents and pesticides. 723 pp. New York, John Wiley & Sons.Google Scholar
El Saidy, M.F., Auda, M. & Degheele, D. (1989) Detoxification mechanisms of diflubenzuron and teflubenzuron in the larvae of Spodoptera littoralis (Boisd.). Pesticide Biochemistry and Physiology 43, 1777–1784.Google Scholar
Falconer, D.S. (1989) Introduction to quantitative genetics, 3rd edn.438 pp. New York, Longman.Google Scholar
Fauziah, I. (1990) Studies on resistance to acylurea compounds in Plutella xylostella L. (Lepidoptera: Yponomeutidae). PhD thesis. 259 pp. University of London.Google Scholar
Fauziah, I. & Wright, D.J. (1992) Synergism of teflubenzuron and chlorfluazuron in an acylurea-resistant field strain of Plutella xylostella L. (Lepidoptera: Yponomeutidae). Pesticide Science 34, 221–226.Google Scholar
Furlong, M.J. & Wright, D.J. (1994) Examination of stability of restistance and cross-resistance patterns to acylurea insect growth regulators in field populations of the diamondback moth, Plutella xylostella, from Malaysia. Pesticide Science 42, 315–326.Google Scholar
Gazit, Y., Ishaaya, I. & Perry, A.S. (1989) Detoxification and synergism of diflubenzuron and chlorfluazuron in the red flour beetle Tribolium castaneum. Pesticide Biochemistry and Physiology 34, 103–110.CrossRefGoogle Scholar
Gunning, R.V., Moores, G.D. & Devonshire, A.L. (1996) Esterases and esfenvalerate resistance in Australian Helicoverpa armigera (Hübner) Lepidoptera, Noctuidae. Pesticide Biochemistry and Physiology 54, 12–23.CrossRefGoogle Scholar
Iqbal, M. & Wright, D.J. (1996) Host resistance to insecticides can confer protection to endo-larval parasitoids. Bulletin of Entomological Research 86, 721–723.CrossRefGoogle Scholar
Iqbal, M., Verkerk, R.H.J., Furlong, M.J., Ong, P-C., Syed, A.R. & Wright, D.J. (1996) Evidence for resistance to Bacillus thuringiensis (Bt) subsp. kurstaki HD-1, Bt subsp. aizawai and abamectin in field populations of the diamondback moth from Malaysia. Pesticide Science 48, 89–97.3.0.CO;2-B>CrossRefGoogle Scholar
Ishaaya, I. & Klein, M. (1990) Response of susceptible laboratory and resistant field strains of Spodoptera littoralis (Lepidoptera: Noctuidae) to teflubenzuron. Journal of Economic Entomology 83, 59–62.CrossRefGoogle Scholar
Lasota, J.A., Shelton, A.M., Bolognese, J.A. & Dybas, R.A. (1996) Toxicity of avermectins to diamondback moth (Lepidoptera: Plutellidae) populations – implications for susceptibility monitoring. Journal of Economic Entomology 89, 33–38.CrossRefGoogle Scholar
McCullagh, P. & Nelder, J.A. (1983) Generalised linear models. London, Chapman & Hall.Google Scholar
Perng, F.S., Yao, M.C. & Sun, C.N. (1988) Susceptibility of diamondback moth (Lepidoptera: Plutellidae) resistant to conventional insecticides to chitin synthesis inhibitors. Journal of Economic Entomology 80, 29–31.CrossRefGoogle Scholar
Rugg, D., Sales, N., Kotze, A.C. & Levot, G.W. (1995) Susceptibility to ivermectin of pyrethroid-resistant Lucilia cuprina (Wiedman) (Diptera: Calliphoridae). Journal of the Australian Entomological Society 34, 69–70.CrossRefGoogle Scholar
Sun, C.N. (1992) Insecticide resistance in diamondback moth. pp. 419–426in Talekar, N.S. (Ed.) Proceedings of the II International Workshop on Management of Diamondback Moth and Other Crucifer Pests. Shanhua, Tainan, 1992. Asian Vegetable Research and Development Center.Google Scholar
Syed, A.R. (1992) Insecticide resistance in diamondback moth in Malaysia. pp. 437–442. in Talekar, N.S. (Ed.) Proceedings of the II International Workshop on Management of Diamondback Moth and Other Crucifer Pests. Shanhua, Tainan, 1992. Asian Vegetable Research and Development Center.Google Scholar
Tabashnik, B.E. (1992) Resistance risk assessment: realized heritability of resistance to Bacillus thuringiensis in diamondback moth (Lepidoptera: Plutellidae), tobacco budworm (Lepidoptera: Noctuidae) and Colorado potato beetle (Coleoptera: Chrysomelidae). Journal of Economic Entomology 85, 1551–1559.CrossRefGoogle Scholar
Van Laecke, K. & Degheele, D. (1991a) Detoxification of diflubenzuron and teflubenzuron in the larvae of the beet armyworm (Spodoptera exigua) (Lepidoptera: Noctuidae). Pesticide Biochemistry and Physiology 40, 181–190.Google Scholar
Van Laecke, K. & Degheele, D. (1991b) Synergism of diflubenzuron and teflubenzuron in larvae of beet armyworm (Lepidoptera: Noctuidae). Journal of Economic Entomology 84, 785–789.CrossRefGoogle Scholar
Verkerk, R.H.J. & Wright, D.J. (1996a) Multitrophic interactions and management of the diamondback moth: a review. Bulletin of Entomological Research 86, 205–216.Google Scholar
Verkerk, R.H.J. & Wright, D.J. (1996b) Field-based studies with the diamondback moth tritrophic system and implications for pest management in the Cameron Highlands, Malaysia. International Journal of Pest Management 43, 27–33.CrossRefGoogle Scholar
Wright, D.J. & Verkerk, R.H.J. (1995) Integration of chemical and biological control systems: evaluation in a multitrophic context. Pesticide Science 44, 207–218.CrossRefGoogle Scholar
Zchorifein, E., Roush, R.T. & Sanderson, J.P. (1994) Potential for integration of biological and chemical control of greenhouse-whitefly (Homoptera: Aleyrodidae) using Encarsia formosa (Hymenoptera: Aphelinidae) and abamectin. Environmental Entomology 23, 1277–1282.Google Scholar