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The effect of insecticide treatment and injection of sodium chloride and sucrose solutions on the water-salt homeostasis of lepidopterous larvae

Published online by Cambridge University Press:  10 July 2009

Yu.V. Natochin ,
R.G. Parnova ,
O.V. Sundukov  and
E.I. Shakhmatova
Yu.V. Natochin*
Affiliation:
Sechenov Institute of Evolutionary Physiology and Biochemistry, St. Petersburg, RU
R.G. Parnova
Affiliation:
Sechenov Institute of Evolutionary Physiology and Biochemistry, St. Petersburg, RU
O.V. Sundukov
Affiliation:
Sechenov Institute of Evolutionary Physiology and Biochemistry, St. Petersburg, RU
E.I. Shakhmatova
Affiliation:
Sechenov Institute of Evolutionary Physiology and Biochemistry, St. Petersburg, RU
*
Prof. Yu.V. Natochin, Sechenov Institute of Evolutionary Physiology and Biochemistry, Thorez pr.44, St. Petersburg 194223, RU.
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Abstract

The osmolality and ion concentrations in the haemolymph and the body of lepidopterous larvae were studied under different experimental conditions. It was shown that the haemolymph osmolality of Aglais urticae (Linnaeus) larvae returned to normal three hours after the injection of 0.4 M sucrose or 0.4 M sodium chloride, despite the fact that in the latter case, the level of sodium was very high (to 53.1 mM vs 4.5 mM in control). The injection of 0.2 M or 0.4 M sodium chloride did not induce significant changes in the concentration of ions, other than sodium, in haemolymph. Treatment of Mamestra brassicae (Linnaeus) larvae with insecticides (DDVP, lindane, decis) led to a fast loss of body weight (up to 30%). This was due to dehydration, which was accompanied by a significant decrease of potassium content in body tissue, but not in the haemolymph. Study of the haemolymph parameters of Lepidoptera and insects taken from their natural environment indicates that the lowest values of variation coefficient related to osmolality and to the concentration of the dominant cation of the haemolymph (sodium, potassium or magnesium). These data suggest effective regulation of osmolality and ion concentration in the insect haemolymph.

Type
Research Paper
Information
Bulletin of Entomological Research , Volume 82 , Issue 2 , June 1992 , pp. 227 - 233
Copyright
Copyright © Cambridge University Press 1992

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References

Bradley, T.J. (1985) The excretory system: structure and physiology. pp. 321–465 in Kerkut, G.A. & Gilbert, L.I. (Eds) Comprehensive insect Physiology, biochemistry and pharmacology. Vol. 4. Oxford, Pergamon Press.Google Scholar
Callec, J.J. (1985) Synaptic transmission in the central nervous system. pp. 139–179in Kerkut, G.A. & Gilbert, L.I. (Eds) Comprehensive insect physiology, biochemistry and pharmacology. Vol. 5. Oxford Pergamon Press.Google Scholar
Casida, J.E., Maddrell, S.H. (1971) Diuretic hormone release on poisoning Rhodnius with insecticide chemicals. Pesticide Biochemistry and Physiology 1, 71–83.CrossRefGoogle Scholar
Crossley, A.C. (1985) Nephrocytes and pericardial cells. pp. 487–515in Kerkut, G.A. & Gilbert, L.I. (Eds) Comprehensive insect physiology, biochemistry and pharmacology. Vol. 4. Oxford, Pergamon Press.Google Scholar
Doherty, J.D. (1985) Insecticides and ion transport. pp. 79–113in Kerkut, G.A. & Gilbert, L.I. (Eds) Comprehensive insect physiology, biochemistry and pharmacology. Vol. 12. Oxford, Pergamon Press.Google Scholar
Eldefrawi, A.T. (1985) Acetylcholinesterases and anticholinesterases. pp. 115–130in Kerkut, G.A. & Gilbert, L.I. (Eds) Comprehensive insect physiology, biochemistry and pharmacology. Vol. 12. Oxford, Pergamon Press.Google Scholar
Florkin, M., Jeuniaux, C. (1974) Hemolymph: composition. pp. 255–307in Rockstein, M. (Ed.) The physiology of Insecta. Vol. 5. London, Academic Press.CrossRefGoogle Scholar
Gerolt, P. (1983) Insecticides: their route of entry, mechanism of transport and mode of action. Biological Reviews of the Cambridge Philosophical Society 58, 233–274.CrossRefGoogle ScholarPubMed
Gourdoux, L., Lequellec, Y., Puiroux, J. & Moreau, R. (1989) Differential hormonal control of hemolymph carbohydrates in Pieris brassicae L. Comparative Biochemistry and Physiology 93, 543–548.CrossRefGoogle Scholar
Lund, A.E. (1984) Pyrethroid modification of sodium channel: current concepts. Pesticide Biochemistry and Physiology 22, 161–168.CrossRefGoogle Scholar
Mullins, D.E. (1985) Chemistry and physiology of the hemolymph. pp. 355–400in Kerkut, G.A. & Gilbert, L.I. (Eds) Comprehensive insect physiology, biochemistry and pharmacology. Vol. 3. Oxford, Pergamon Press.Google Scholar
Natochin, Yu.V. & Parnova, R.G. (1987) Osmolality and electrolyte concentration of hemolymph and the problem of ion and volume regulation of cells in higher insects. Comparative Biochemistry and Physiology 88A, 563–570.CrossRefGoogle Scholar
Natochin, Yu.V., Rjabov, S.I., Kajukov, I.G., Lavrova, E.A., Monin, Yu.G., Shakhmatova, E.I. & Emanuel, V.L. (1980) Indices of water-salt homeostasis and their variability. Fisiologia cheloveka 6, 647–650. [in Russian].Google Scholar
Natochin, Yu.V., Parnova, R.G., Sundukov, O.V. & Shakhmatova, E.I. (1989) The loss of electrolytes as a cause of insect dehydration under insecticide action. Doklady Academia Nauk SSSR 305, 752–754. [in Russian].Google Scholar
Phillips, J. (1981) Comparative physiology of insect renal function. American Journal of Physiology 241, R241–R257.Google ScholarPubMed
Pichon, Y., Guillet, J.C., Heilig, U. & Pelhate, M. (1985) Recent studies on the effects of DDT and pyrethroid insecticides on nervous activity in cockroaches. Pesticide Science 16, 627–640.CrossRefGoogle Scholar
Prosser, C.L. & Brown, F.A., (1962) Comparative animal physiology. 688 pp. Philadelphia, W.B. Saunders Company.Google Scholar