Hostname: page-component-78c5997874-94fs2 Total loading time: 0 Render date: 2024-11-03T02:23:20.319Z Has data issue: false hasContentIssue false
  • English
  • Français

DNA barcoding and real-time PCR detection of Bactrocera xanthodes (Tephritidae: Diptera) complex

Published online by Cambridge University Press:  06 May 2018

D. Li ,
D.W. Waite ,
D.N. Gunawardana ,
B. McCarthy ,
D. Anderson ,
A. Flynn  and
S. George
D. Li*
Affiliation:
Plant Health and Environment Laboratory, Ministry for Primary Industries, P.O. Box 2095, Auckland 1140, New Zealand
D.W. Waite
Affiliation:
Plant Health and Environment Laboratory, Ministry for Primary Industries, P.O. Box 2095, Auckland 1140, New Zealand Australian Centre for Ecogenomics (ACE), School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia 4072, Australia
D.N. Gunawardana
Affiliation:
Plant Health and Environment Laboratory, Ministry for Primary Industries, P.O. Box 2095, Auckland 1140, New Zealand
B. McCarthy
Affiliation:
Plant Health and Environment Laboratory, Ministry for Primary Industries, PO Box 14018, Christchurch 8544, New Zealand.
D. Anderson
Affiliation:
Plant Health and Environment Laboratory, Ministry for Primary Industries, PO Box 14018, Christchurch 8544, New Zealand.
A. Flynn
Affiliation:
Plant Health and Environment Laboratory, Ministry for Primary Industries, P.O. Box 2095, Auckland 1140, New Zealand
S. George
Affiliation:
Plant Health and Environment Laboratory, Ministry for Primary Industries, P.O. Box 2095, Auckland 1140, New Zealand
*
*Author for correspondence Phone: +64 9 9098622 Fax: +64 9 909 5739 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Immature fruit fly stages of the family Tephritidae are commonly intercepted on breadfruit from Pacific countries at the New Zealand border but are unable to be identified to the species level using morphological characters. Subsequent molecular identification showed that they belong to Bactrocera xanthodes, which is part of a species complex that includes Bactrocera paraxanthodes, Bactrocera neoxanthodes and an undescribed species. To establish a more reliable molecular identification system for B. xanthodes, a reference database of DNA barcode sequences for the 5’-fragment of COI gene region was constructed for B. xanthodes from Fiji, Samoa and Tonga. To better understand the species complex, B. neoxanthodes from Vanuatu and B. paraxanthodes from New Caledonia were also barcoded. Using the results of this analysis, real-time TaqMan polymerase chain reaction (PCR) assays for the detection of B. xanthodes complex and for the three individual species of the complex were developed and validated. The assay showed high specificity for the target species, with no cross-reaction observed for closely related organisms. Each of the real-time PCR assays is sensitive, detecting the target sequences at concentrations as low as ten copies µl−1 and can be used as either singleplex or multiplex formats. This real-time PCR assay for B. xanthodes has been successfully applied at the borders in New Zealand, leading to the rapid identification of intercepted Tephritidae eggs and larvae. The developed assays will be useful biosecurity tools for rapid detection of species in the B. xanthodes complex worldwide.

Keywords

B. xanthodesB. neoxanthodesB. paraxanthodesbiosecuritydiagnosticsinterceptions
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 109 , Issue 1 , February 2019 , pp. 102 - 110
Check for updates
Copyright
Copyright © Cambridge University Press 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Allwood, A., Tumukon, T., Tau, D. & Kassim, A. (1997) Fruit fly fauna in Vanuatu. pp. 70–80 in Pacific, Allwood, A. & Drew, R.A.I. (Eds) Management of Fruit Flies in the a Regional Symposium, Nadi, Fiji. 28–31 October 1996. ACIAR Proceedings No. 76. Melbourne, Brown, Prior and Anderson.Google Scholar
Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. (1990) Basic local alignment search tool. Journal of Molecular Biology 215, 403–410.Google Scholar
Armstrong, K. F. & Ball, S. L. (2005) DNA barcodes for biosecurity: invasive species identification. Philosophical Transactions of the Royal Society B: Biological Sciences 360, 1813–1823.Google Scholar
Armstrong, K. F., Cameron, C. M. & Frampton, E. R. (1997) Fruit fly (Diptera: Tephritidae) species identification: a rapid molecular diagnostic technique for quarantine application. Bulletin of Entomological Research 87, 111–118.Google Scholar
Asokan, R., Rebijith, K. B., Singh, S. K., Sidhu, A. S., Siddharthan, S., Karanth, P. K., Ellango, R. & Ramamurthy, V. V. (2011) Molecular identification and phylogeny of Bactrocera species (Diptera: Tephritidae). Florida Entomologist 94, 1026–1035.Google Scholar
Blacket, M. J., Semeraro, L. & Malipatil, M. B. (2012) Barcoding Queensland Fruit Flies (Bactrocera tryoni): impediments and improvements. Molecular Ecology Resources 12, 428–436. doi: 10.1111/j.1755-0998.2012.03124.x.Google Scholar
Bustin, S. A., Benes, V., Garson, J. A., Hellemans, J., Huggett, J., Kubista, M., Mueller, R., Nolan, T., Pfaffl, M. W., Shipley, G. L., Vandesompele, J. & Wittwer, C. T. (2009) The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clinical Chemistry 55, 611–622.Google Scholar
Chua, T. H., Chong, Y. V. & Lim, S. H. (2010) Species determination of Malaysian Bactrocera pests using PCR-RFLP analyses (Diptera: Tephritidae). Pest Management Science 66, 379–384. doi: 10.1002/ps.1886.Google Scholar
Cowley, J. M., Baker, R. T., Englberger, K. G. & Lang, T. G. (1991) Methyl Bromide Fumigation of Tongan Watermelons against Bactrocera xanthodes (Diptera: Tephritidae) and Analysis of Quarantine Security. Journal of Economic Entomology 84, 1763–1767.Google Scholar
Dhami, M. K., Gunawardana, D. N., Voice, D. & Kumarasinghe, L. (2016) A real-time PCR toolbox for accurate identification of invasive fruit fly species. Journal of Applied Entomology 140, 536–552.Google Scholar
Drew, R. A. & Romig, M. C. (2001) The fruit fly fauna (Diptera: Tephritidae: Dacinae) of Bougainville, the Solomon Islands and Vanuatu. Austral Entomology 40,113–150.Google Scholar
Drew, R. A. I. & Hancock, D. L. (1995) New Species, Subgenus and Records of Bactrocera Macquart from the South Pacific (Diptera: Tephritidae: Dacinae). Australian Journal of Entomology 34, 7–12.Google Scholar
Drew, R. A. I., AlIwood, A. J. & Tau, D. (1997) Bactrocera paraxanthodes Drew and Hancock – an example of how host records and attractant responses contribute to taxonomic research. pp. 131–133 in Allwood, A. & Drew, R.A.I. (Eds) Management of Fruit Flies in the Pacific. A Regional Symposium, Nadi, Fiji. 28–31 October 1996. ACIAR Proceedings No. 76. Melbourne, Brown, Prior and Anderson.Google Scholar
Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology Biotechnology 3, 294–299.Google Scholar
Hebert, P. D. N., Ratnasingham, S. & deWaard, J. R. (2003) Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings of the Royal Society B: Biological Sciences 270, S96–S99.Google Scholar
Heimoana, V., Thnupopo, F., Toleafoa, E. & Fakanaiki, C. (1997) Fruit Fly Fauna of Tonga, Western Samoa, American, Samoa and Niue. pp. 57–59 in Allwood, A. & Drew, R.A.I. (Eds) Management of Fruit Flies in the Pacific. A Regional Symposium, Nadi, Fiji. 28–31 October 1996. ACIAR Proceedings No. 76. Melbourne, Brown, Prior and Anderson.Google Scholar
Hoeben, P., Daniel, L. J., Ma, J. & Drew, R. A. I. (1996) The Bactrocera (Notodacus) xanthodes (Broun) species complex (Diptera: Tephritidae): comparison of 18S rRNA sequences from Fiji, Tonga and Vanuatu specimens suggest two distinct strains. Australian Journal of Entomology 35, 61–64.Google Scholar
Jamnongluk, W., Baimai, V. & Kittayapong, P. (2003) Molecular phylogeny of tephritid fruit flies in the Bactrocera tau complex using the mitochondrial COI sequences. Genome 46, 112–118. doi: 10.1139/g02-113.Google Scholar
Jiang, F., Li, Z. H., Deng, Y. L., Wu, J. J., Liu, R. S. & Buahom, N. (2013) Rapid diagnosis of the economically important fruit fly, Bactrocera correcta (Diptera: Tephritidae) based on a species-specific barcoding cytochrome oxidase I marker. Bulletin of Entomology Research 103, 363–371. doi: 10.1017/S0007485312000806.Google Scholar
Jiang, F. Q., Liang, J. L., Zhang, A. B. & Li, Z. H. (2014) Existence of species complex largely reduced barcoding success for invasive species of Tephritidae: a case study in Bactrocera spp. Molecular Ecology Resources 14, 1114–1128.Google Scholar
Kunprom, C. & Pramual, P. (2016) DNA barcode variability and host plant usage of fruit flies (Diptera: Tephritidae) in Thailand. Genome 59, 792–804. doi: 10.1139/gen-2015-0110.Google Scholar
Leblanc, L., Vueti, E. T., Drew, R. A. & Allwood, A. J. (2012) Host plant records for fruit flies (Diptera: Tephritidae: Dacini) in the Pacific Islands. Proceedings of the Hawaiian Entomological Society 44, 11–53.Google Scholar
Leblanc, L., San Jose, M., Barr, N. & Rubinoff, D. (2015) A phylogenetic assessment of the polyphyletic nature and intraspecific color polymorphism in the Bactrocera dorsalis complex (Diptera, Tephritidae). Zookeys 26, 339–367.Google Scholar
Li, D., Fan, Q.-H., Waite, D. W., Gunawardana, D., George, S. & Kumarasinghe, L. (2015) Development and Validation of a Real-Time PCR Assay for Rapid Detection of Two-Spotted Spider Mite, Tetranychus urticae (Acari: Tetranychidae). PLoS ONE 10(7), e0131887.Google Scholar
Meeyen, K., Sopaladawan, P. N. & Pramual, P. (2014) Population structure, population history and DNA barcoding of fruit fly Bactrocera latifrons (Hendel) (Diptera: Tephritidae). Entomological Science 17, 219–230. doi: 10.1111/ens.12049.Google Scholar
OligoAnalyzer (2012) PrimerQuest® program. 3.1. Retrieved 12 December, 2012. Available online at http://www.idtdna.com/Scitools.Google Scholar
Plant Health Australia (2016) The Australian Handbook for the Identification of Fruit Flies version 2.1. 1/1 Phipps Close Deakin ACT 2600, Plant Health Australia.Google Scholar
R Development Core Team (2011) R: A Language and Environment for Statistical Computing. Available online at http://www.R-project.org/.Google Scholar
Stephenson, B.P., Gill, G.S.C., Ran, J.L. & Wilson, J. A. (2003) Biosecurity approaches to surveillance and response for new plant pest species. New Zealand Plant Protection 56, 5–9.Google Scholar
Tora Vueti, E., Allwood, A., Leweniqila, L., Ralulu, L., Balawakula, A., Malau, A., Sales, F. & Peleti, K. (1997 a) Fruit fly fauna in Fiji, Tuvalu, Wallis and Futuna, Tokelau and Nauru. pp. 60–63 in Pacific, A. & Drew, R.A.I. (Eds) Management of Fruit Flies in the A Regional Symposium, Nadi, Fiji. 28–31 October 1996. ACIAR Proceedings No. 76. Melbourne, Brown, Prior and Anderson.Google Scholar
Tora Vueti, E., Ralulu, L., Walker, G., Allwood, A., Leweniqila, L. & Balawakula, A. (1997 b) Host availability – its impact on seasonal abundance of fruit flies. pp. 105–110 in Pacific, A. & Drew, R.A.I. (Eds) Management of Fruit Flies in the A Regional Symposium, Nadi, Fiji. 28–31 October 1996. ACIAR Proceedings No. 76. Melbourne, Brown, Prior and Anderson.Google Scholar