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Differentiation of anopheles culicifacies Giles (Diptera: Culicidae) sibling species by analysis of cuticular components

Published online by Cambridge University Press:  10 July 2009

P. J. M. Milligan ,
A. Phillips ,
D. H. Molyneux ,
S. K. Subbarao  and
G. B. White
P. J. M. Milligan
Affiliation:
Department of Biological Sciences, University of Salford, Salford, M5 4WT, UK
A. Phillips
Affiliation:
Department of Biological Sciences, University of Salford, Salford, M5 4WT, UK
D. H. Molyneux
Affiliation:
Department of Biological Sciences, University of Salford, Salford, M5 4WT, UK
S. K. Subbarao
Affiliation:
Malaria Research Centre, Indian Council for Medical Research, 22 Sham Nath Marg, Delhi-1100054, India
G. B. White
Affiliation:
Department of Entomology, London School of Hygiene & Tropical Medicine, London, WC1E 7HT, UK
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Abstract

Cuticular wax was extracted from adult females of Anopheles culicifacies Giles and analysed by gas liquid chromatography. The flies were taken from pure cage stocks of three sibling species: 56 individuals of species A, 43 of species B and 51 of species C. The three cytospecies were found to be significantly different in their cuticular hydrocarbon composition by a multivariate analysis of variance. Using discriminant analysis, each cytospecies was characterized by the amounts of C25-C33 hydrocarbons extracted from the cuticle, allowing criteria to be established for the identification of flies. By these criteria, specimens from stocks of known identity were allocated to the correct group with a high success rate. These results may reflect ecological differences among the sibling species.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 76 , Issue 3 , September 1986 , pp. 529 - 537
Copyright
Copyright © Cambridge University Press 1986

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References

Akoh, J. I., Beidas, M. F. & White, G. B. (1984). Cytotaxonomic evidence for the malaria vector species A of the Anopheles culicifacies complex being endemic in Arabia.—Trans. R. Soc. trop. Med. Hyg. 78, 698.Google Scholar
Box, G. E. P. & Cox, D. R. (1964). An analysis of transformations.—Jl R. statist. Soc. (B) 26, 211–252.Google Scholar
Carlson, D. A. (1984). Chemical taxonomy: analysis of cuticular hydrocarbons for identification of Simulium, Anopheles and Glossina species.—pp. 131–150 in Newton, B. N. & Michal, F. (Eds.). New approaches to the identification of parasites and their vectors. Proceedings of a Symposium on Application of Biochemical and Molecular Biology Techniques to Problems of Parasite and Vector Identification. Geneva, 8–10 November 1982.—466 pp. Basle, Schwabe. (UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases. Tropical Research Series no. 5).Google Scholar
Carlson, D. A. & Service, M. W. (1979). Differentiation between species of the Anopheles gambiae Giles complex (Diptera: Culicidae) by analysis of cuticular hydrocarbons.—Ann. trop. Med. Parasit. 73, 589–592.CrossRefGoogle ScholarPubMed
Carlson, D. A. & Service, M. W. (1980). Identification of mosquitoes of Anopheles gambiae species complex A and B by analysis of cuticular components.—Science, N.Y. 207, 1089–1091.CrossRefGoogle Scholar
Carlson, D. A. & Walsh, J. F. (1981). Identification of two West African black flies (Diptera: Simuliidae) of the Simulium damnosum species complex by analysis of cuticular paraffins.—Acta trop. 38, 235–239.Google ScholarPubMed
Green, C. A. & Miles, S. J. (1980). Chromosomal evidence for sibling species of the malaria vector Anopheles (Cellia) culicifacies Giles.—J. trop. Med. Hyg. 83, 75–78.Google ScholarPubMed
Hamilton, R. J. & Service, M. W. (1983). Value of cuticular and internal hydrocarbons for the identification of larvae of Anopheles gambiae Giles, Anopheles arabiensis Patton and Anopheles melas Theobald.—Ann. trop. Med. Parasit. 77, 203–210.CrossRefGoogle ScholarPubMed
Kendall, M., Stuart, A. & Ord, J. K. (1983). The advanced theory of statistics. Vol. III: design and analysis, and time series.—4th edn, 780 pp. London, Charles Griffin.Google Scholar
Lane, R., Phillips, A., Molyneux, D. H., Procter, G. & Ward, R. D. (1985). Chemical analysis of the abdominal glands of two forms of Lutzomyia longipalpis: site of a possible sex pheromone?—Ann. trop. Med. Parasit. 79, 225–229.CrossRefGoogle ScholarPubMed
Mahmood, F., Sakai, R. K. & Akhtar, K. (1984). Vector incrimination studies and observations on species A and B of the taxon Anopheles culicifacies in Pakistan.—Trans. R. Soc. trop. Med. Hyg. 78, 607–616.CrossRefGoogle Scholar
Morrison, D. F. (1976). Multivariate statistical methods.—2nd ed, 416 pp. New York, Wiley.Google Scholar
Newton, B. N. & Michal, F. (1984). New approaches to the identification of parasites and their vectors. Proceedings of a Symposium on Application of Biochemical and Molecular Biology Techniques to Problems of Parasite and Vector Identification. Geneva, 8–10 11 1982.—466 pp. Basle, Schwabe. (UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases. Tropical Research Series no. 5).Google Scholar
Phillips, A., Walsh, J. F., Garms, R., Molyneux, D. H., Milligan, P. & Ibrahim, G. (1985). Identification of adults of the Simulium damnosum complex using hydrocarbon analysis.—Tropical Medicine & Parasitology 36, 97–101.Google ScholarPubMed
Reuben, R., Mani, T. R. & Tewari, S. C. (1984). Feeding behaviour, age structure and vectorial capacity of Anopheles culicifacies Giles along the river Thenpennai (Tamil Nadu).—Indian J. med. Res. 80, 23–29.Google ScholarPubMed
Sabesan, S., Jambulingam, P., Krishnamoorthy, K., Vijayan, V. A., Gunasekaran, K., Rajendran, G., Chandrahas, R. K. & Rajagopalan, P. K. (1984). Natural infection and vectorial capacity of Anopheles culicifacies Giles in Rameswaram Island (Tamil Nadu).—Indian J. med. Res. 80, 43–46.Google ScholarPubMed
Subbarao, S. K. (1984). Biological species in malaria vectors of India.—pp. 77–83 in Sharma, V. P. (Ed.). Indo-UK workshop on malaria. Proceedings of the workshop held at the Indian Council of Medical Research, New Delhi, 11 14–19, 1983.—276 pp. Delhi, Malaria Research Centre.Google Scholar
Subbarao, S. K., Adak, T. & Sharma, V. P. (1980). Anopheles culicifacies: sibling species distribution and vector incrimination studies.—J. Commun. Dis. 12, 102–104.Google ScholarPubMed
Subbarao, S. K., Vasantha, K., Adak, T. & Sharma, V. P. (1983). Anopheles culicifacies complex: evidence for a new sibling species, species C.—Ann. ent. Soc. Am. 76, 985–988.CrossRefGoogle Scholar
Suguna, S. G., Tewari, S. C., Mani, T. R., Hiriyan, J. & Reuben, R. (1983). Anopheles culicifacies species complex in Thenpennaiyar riverine tract, Tamil Nadu.—Indian J. med. Res. 77, 455–459.Google ScholarPubMed
Tewari, S. C., Mani, T. R., Saguna, S. G. & Reuben, R. (1984). Host selection patterns in anophelines in riverine villages of Tamil Nadu.—Indian J. Med. Res. 80, 18–22.Google ScholarPubMed
Townson, H., Post, R. & Phillips, A. (in press). Biochemical approaches to blackfly taxonomy.—in Kim, E. C. (Ed.). Proceedings of the International Conference on Ecology and Population Management of Blackflies.Google Scholar
Vasantha, K., Subbarao, S. K., Adak, T. & Sharma, V. P. (1982). Karyotypic variations in Anopheles culicifacies complex.—Indian J. Malar. 19, 27–32.Google Scholar
Vasantha, K., Subbarao, S. K., Adak, T. & Sharma, V. P. (1983). Anopheles culicifacies: mitotic karyotype of species C.—Indian J. Malar. 20, 161–162.Google Scholar
White, G. B. (1974). Anopheles gambiae complex and disease transmission in Africa.—Trans. R. Soc. trop. Med. Hyg. 68, 278–301.CrossRefGoogle ScholarPubMed
White, G. B. (1978). Systematic reappraisal of the Anopheles maculipennis complex.—Mosq. Syst. 10, 13–44.Google Scholar
White, G. B. (1985). Anopheles bwambae sp. n., a malaria vector in the Semliki Valley, Uganda, and its relationships with other sibling species of the An. gambiae complex (Diptera: Culicidae).—Syst. Entomol. 10, 501–522.CrossRefGoogle Scholar
Adak, T.Subbaroa, S. K. & Sharma, V. P. (1983). Inheritance pattern of vermilion-eye in Anopheles culicifacies species A.—Indian J. Malar. 20, 59–61.Google Scholar