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The dietary arachidonic acid improved growth and immunity of honey bee (Apis mellifera ligustica)

Published online by Cambridge University Press:  08 October 2021

Jing Yu Open the ORCID record for Jing Yu [Opens in a new window] ,
Weixing Zhang ,
Xuepeng Chi ,
Wenfeng Chen ,
Zhenfang Li ,
Ying Wang ,
Zhenguo Liu ,
Hongfang Wang Open the ORCID record for Hongfang Wang [Opens in a new window]  and
Baohua Xu
Jing Yu
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Tai'an, China
Weixing Zhang
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Tai'an, China
Xuepeng Chi
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Tai'an, China
Wenfeng Chen
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Tai'an, China
Zhenfang Li
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Tai'an, China
Ying Wang*
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Tai'an, China
Zhenguo Liu
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Tai'an, China
Hongfang Wang*
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Tai'an, China
Baohua Xu*
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Tai'an, China
*
Author for correspondence: Baohua Xu, Email: [email protected]; Hongfang Wang, Email: [email protected]
Author for correspondence: Baohua Xu, Email: [email protected]; Hongfang Wang, Email: [email protected]
Author for correspondence: Baohua Xu, Email: [email protected]; Hongfang Wang, Email: [email protected]
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Abstract

Honeybees cannot synthesize arachidonic acid (ARA) themselves, only obtain it from food. Most pollen is deficient or contains a small amount of ARA. The necessity of supplementary ARA in bees’ diet has not been studied. The objective of this study was to investigate the effects of dietary ARA levels on the growth and immunity of Apis mellifera ligustica. A total of 25 honeybee colonies were randomly assigned to five dietary groups which were fed basic diets supplemented with 0, 2, 4, 6, and 8% of ARA. The diet with 4% ARA improved the body weight of newly emerged worker bees compared with the control group. Supplement of ARA in honeybee diets changed the fatty acid composition of honeybee body. SFA and MUFA contents of bees’ body declined, and PUFA content rised in the ARA group. Compared with the control group, the supplement of ARA in honeybee diets increased the contents of ARA, C22:6n-3 (DHA) and C18:3n-6 in bees’ body significantly, but decreased the contents of C16:1 and C18:3n-3. The diet supplied with 4% ARA reduced the mortality rate of honeybee infected with Escherichia coli. The activity of immune enzymes (phenoloxidase, antitrypsin, and lysozyme) and the mRNA expression levels of immune genes (defensin-2, toll, myd88, and dorsal) were improved by ARA diets to varying degrees depending on the ARA levels, especially 4% ARA. These results suggested that dietary ARA could improve the growth, survival, and immune functions of honeybees. Supplement of ARA in bees’ diet would be valuable for the fitness of honeybees.

Keywords

Arachidonic acidfatty acidsgrowthhoney beeimmune functions
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 112 , Issue 2 , April 2022 , pp. 261 - 270
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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