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CYP4CJ6-mediated resistance to two neonicotinoid insecticides in Sitobion miscanthi (Takahashi)

Published online by Cambridge University Press:  17 February 2022

Gui-Lei Hu ,
Liu-Yang Lu ,
Ya-She Li ,
Xu Su ,
Wen-Yang Dong ,
Bai-Zhong Zhang Open the ORCID record for Bai-Zhong Zhang [Opens in a new window] ,
Run-Qiang Liu ,
Ming-Wang Shi ,
Hong-Liang Wang  and
Xi-Ling Chen
Gui-Lei Hu
Affiliation:
College of Resources and Environment, Henan Engineering Research Center of Biological Pesticide & Fertilizer Development and Synergistic Application, Henan Institute of Science and Technology, Xinxiang 453003, P.R. China
Liu-Yang Lu
Affiliation:
College of Resources and Environment, Henan Engineering Research Center of Biological Pesticide & Fertilizer Development and Synergistic Application, Henan Institute of Science and Technology, Xinxiang 453003, P.R. China
Ya-She Li
Affiliation:
College of Resources and Environment, Henan Engineering Research Center of Biological Pesticide & Fertilizer Development and Synergistic Application, Henan Institute of Science and Technology, Xinxiang 453003, P.R. China
Xu Su
Affiliation:
College of Resources and Environment, Henan Engineering Research Center of Biological Pesticide & Fertilizer Development and Synergistic Application, Henan Institute of Science and Technology, Xinxiang 453003, P.R. China
Wen-Yang Dong
Affiliation:
Department of Entomology, China Agricultural University, Beijing 100193, P.R. China
Bai-Zhong Zhang*
Affiliation:
College of Resources and Environment, Henan Engineering Research Center of Biological Pesticide & Fertilizer Development and Synergistic Application, Henan Institute of Science and Technology, Xinxiang 453003, P.R. China
Run-Qiang Liu
Affiliation:
College of Resources and Environment, Henan Engineering Research Center of Biological Pesticide & Fertilizer Development and Synergistic Application, Henan Institute of Science and Technology, Xinxiang 453003, P.R. China
Ming-Wang Shi
Affiliation:
College of Resources and Environment, Henan Engineering Research Center of Biological Pesticide & Fertilizer Development and Synergistic Application, Henan Institute of Science and Technology, Xinxiang 453003, P.R. China
Hong-Liang Wang
Affiliation:
College of Resources and Environment, Henan Engineering Research Center of Biological Pesticide & Fertilizer Development and Synergistic Application, Henan Institute of Science and Technology, Xinxiang 453003, P.R. China
Xi-Ling Chen
Affiliation:
College of Resources and Environment, Henan Engineering Research Center of Biological Pesticide & Fertilizer Development and Synergistic Application, Henan Institute of Science and Technology, Xinxiang 453003, P.R. China
*
Author for correspondence: Bai-Zhong Zhang, Email: [email protected]
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Abstract

The wheat aphid Sitobion miscanthi (CWA) is an important harmful pest in wheat fields. Insecticide application is the main method to effectively control wheat aphids. However, CWA has developed resistance to some insecticides due to its extensive application, and understanding resistance mechanisms is crucial for the management of CWA. In our study, a new P450 gene, CYP4CJ6, was identified from CWA and showed a positive response to imidacloprid and thiamethoxam. Transcription of CYP4CJ6 was significantly induced by both imidacloprid and thiamethoxam, and overexpression of CYP4CJ6 in the imidacloprid-resistant strain was also observed. The sensitivity of CWA to these two insecticides was increased after the knockdown of CYP4CJ6. These results indicated that CYP4CJ6 could be associated with CWA resistance to imidacloprid and thiamethoxam. Subsequently, the posttranscriptional regulatory mechanism was assessed, and miR-316 was confirmed to participate in the posttranscriptional regulation of CYP4CJ6. These results are crucial for clarifying the roles of P450 in the resistance of CWA to insecticides.

Keywords

CYP4CJ6insecticidesmiR-316RNAiSitobion miscanthi
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 112 , Issue 5 , October 2022 , pp. 646 - 655
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Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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Footnotes

*

These authors contributed equally to this work.

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