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Comparison of microplate esterase assays and immunoassay for identifying insecticide resistant variants of Myzus persicae (Homoptera: Aphididae)

Published online by Cambridge University Press:  10 July 2009

Alan L. Devonshire ,
Gregor J. Devine  and
Graham D. Moores
Alan L. Devonshire*
Affiliation:
AFRC, Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, UK
Gregor J. Devine
Affiliation:
AFRC, Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, UK
Graham D. Moores
Affiliation:
AFRC, Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, UK
*
Alan L. Devonshire, AFRC, Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden Herts, AL5 2JQ, UK
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Abstract

Insecticide resistant Myzus persicae (Sulzer) employ increased esterase activity to detoxify insecticides by hydrolysis and sequestration. The amount of esterase, and hence resistance, in individual aphids can be determined by measuring either overall naphthyl acetate hydrolysis in crude homogenates, or the specific enzyme responsible (E4 or FE4) after electrophoresis or immunological isolation. The ability of a total esterase assay, done in microplates, to discriminate between susceptible (S) aphids and resistant variants (R1, R2) with different amounts of E4/FE4, was compared with the resolving power of the more elaborate immunoassay technique. The immunoassay gave the better discrimination between variants, resolving them all with greater than 95% confidence, with particularly good separation of R1, from S. The microplate assay using crude homogenates, although a poorer discriminator, identified most of the very resistant (R2) aphids, and provided a robust and widely accessible method for broadly representing the resistance of field populations.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 82 , Issue 4 , December 1992 , pp. 459 - 463
Copyright
Copyright © Cambridge University Press 1992

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References

Blackman, R.L. (1988) Rearing and handling aphids. pp. 59–68 in: Aphids, their Biology, Natural Enemies and Control Vol. B. (Eds). Minks, A.K. & Harrewijn, P.Amsterdam, Elsevier Science Publishers.Google Scholar
Brown, T.M. & Brogdon, W.G. (1987) Improved detection of in secticide resistance through conventional and molecular techniques. Annual Review of Entomology 32, 145–162.Google Scholar
Devonshire, A.L. (1975) Studies of the carboxylesterases of Myzus persicae resistant and susceptible to organophosphorus insecticides. Proceedings 8th British Insecticide and Fungicide Conference 1, 67–73.Google Scholar
Devonshire, A.L. (1977) The properties of a carboxylesterase from the peach-potato aphid Myzus persicae (Sulz.), and its role in conferring insecticide resistance. Biochemical Journal 167, 675–683.CrossRefGoogle ScholarPubMed
Devonshire, A.L. (1989) Resistance of aphids to insecticides. pp. 123–139 in: Aphids, their biology, natural enemies and control Vol. B. (Eds) Minks, A.K. & Harrewijn, P., Amsterdam, Elsevier Science Publishers.Google Scholar
Devonshire, A.L. & Moores, G.D. (1982) A carboxylesterase with broad substrate specificity causes organophosphorus, carbamate and pyrethroid resistance in peach-potato aphids (Myzus persicae).Pesticide Biochemistry and Physiology 18, 235–246.Google Scholar
Devonshire, A.L., Moores, G.D. & ffrench-Constant, R.H. (1986) Detection of insecticide resistance by immunological estimation of carboxylesterase activity in Myzus persicae (Sulzer) and cross reaction of the antiserum with Phorodon humuli (Schrank) (Hemiptera: Aphididae). Bulletin of Entomological Research 76, 97–107.CrossRefGoogle Scholar
ffrench-Constant, R.H. & Devonshire, A.L. (1987) A multiple homogeniser for rapid sample preparation in immunoas says and electrophoresis. Biochemical Genetics 25, 493–499.Google Scholar
ffrench-Constant, R.H. & Devonshire, A.L. (1988) Monitoring frequencies of insecticide resistance in Myzus persicae (Sulzer) (Hemiptera: Aphididae) in England during 1985–1986 by immunoassay. Bulletin of Entomological Research 78, 163–171.Google Scholar
ffrench-Constant, R.H., Clark, S.J. & Devonshire, A.L. (1988) Effect of decline of insecticide residues on selection for insecticide resistance in Myzus persicae (Sulzer) (Hemiptera: Aphididae). Bulletin of Entomological Research 78, 19–29.Google Scholar
Field, L.M.Devonshire, A.L. & Forde, B.G. (1988) Molecular evidence that insecticide resistance in peach-potato aphids (Myzus persicae, (Sulz.)) results from amplification of an esterase gene. Biochemical Journal 251, 309–312.Google Scholar
Grant, D.F., Bender, D.M. & Hammock, B.D. (1989) Quantitative kinetic assays for glutathione S-transferase and general esterase in individual mosquitoes using an EIA reader. Insect Biochemistry 19, 741–751.Google Scholar
Needham, P.H. & Sawicki, R.M. (1971) Diagnosis of resistance to organophosphorus insecticides in Myzus persicae (Sulz.) Nature, London 230, 125–126.Google Scholar
Sawicki, R.M., Devonshire, A.L., Payne, R.W. & Petzing, S.M. (1980) Stability of insecticide resistance in the peach-potato aphid, Myzus persicae (Sulzer). Pesticide Science 11, 33–42.Google Scholar
Smith, S.D.J., Dewar, A.M. & Devonshire, A.L. (1990). Resistance of Myzus persicae to insecticides applied to sugar beet. Proceedings of IIRB 53rd Winter Congress, 379–398.Google Scholar
Wachendorff, U. & Klingauf, F. (1978) An esterase assay for the diagnosis of resistance in aphids. Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz 85, 218–227.Google Scholar