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Changes occurred in the testes and DNA pattern of males wax moth (Galleria mellonella) first generation as a result of irradiation of their parents

Published online by Cambridge University Press:  15 December 2016

Salwa Abdo Rizk ,
Ragaa Sayed Abdalla  and
Rehab Mahmoud Sayed
Salwa Abdo Rizk
Affiliation:
Natural Product Department, National Center of Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt
Ragaa Sayed Abdalla
Affiliation:
Natural Product Department, National Center of Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt
Rehab Mahmoud Sayed*
Affiliation:
Natural Product Department, National Center of Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt
*
*Author for correspondence Phone: 01156662204 Fax: (+202) 22749298 E-mail: [email protected]
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Abstract

Nowadays, the sterile insect technique is broadly used as a pest control measure. Therefore, the present study was conducted to investigate the alteration occurred in testes and DNA pattern as an effect of inherited sterility. Full grown pupae of the wax moth, Galleria mellonella were irradiated with 80 and 160 Gy of γ irradiation. The size of the testes was decreased by increasing of γ irradiation dose. Also, the size of the testes was decreased in F1 males comparing with the size of the testes of both the parents and the untreated control. The effects of γ rays on the DNA patterns of adult male parents and F1 males showed alterations among the controls, the treated parents and F1 individuals. Exposure to radiation caused very frequently the appearance of some extra bands and the deficiency of others in the arbitrary random amplified polymorphic DNA-polymerase chain reaction amplification patterns of the irradiated insects.

Keywords

wax mothmale reproductive systemRAPD-PCRγ radiation
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 107 , Issue 4 , August 2017 , pp. 493 - 498
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Copyright
Copyright © Cambridge University Press 2016 

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