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A biochemical and toxicological study of the role of insensitive acetylcholinesterase in organophosphorus resistant Bemisia tabaci (Homoptera: Aleyrodidae) from Israel

Published online by Cambridge University Press:  10 July 2009

Frank J. Byrne ,
Matthew Cahill ,
Ian Denholm  and
Alan L. Devonshire
Frank J. Byrne*
Affiliation:
Department of Biological & Ecological Chemistry, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, UK.
Matthew Cahill
Affiliation:
Department of Biological & Ecological Chemistry, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, UK.
Ian Denholm
Affiliation:
Department of Biological & Ecological Chemistry, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, UK.
Alan L. Devonshire
Affiliation:
Department of Biological & Ecological Chemistry, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, UK.
*
Department of Biological and Ecological Chemistry, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Herts AL5 2JQ, UK.
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Abstract

Two acetylcholinesterase (AChE) variants, differing in sensitivity to inhibition by the organophosphorus (OP) insecticide paraoxon were identified in a population of Bemisia tabaci (Gennadius) from cotton in Israel using a single insect kinetic microplate assay. Two strains were established, homogeneous for one or other of the two variants, by isolating mated females from the field population onto individual cotton leaves, and testing a proportion of their female offspring to identify their AChE genotype. Polyacrylamide gel electrophoresis of their I-naphthyl butyrate hydrolyzing esterases showed that all insects contained esterase E0 14, which is indicative of B-type whiteflies, although the staining intensity of this band differed. Resistance to the OPs monocrotophos, profenofos and chlorpyrifos in leaf dip bioassays was consistent with the presence of the insensitive AChE. The data also indicated that separate mechanisms conferred resistance to the two pyrethroids cypermethrin and bifenthrin. The former, when used in a mixture with profenofos, was no more toxic than when the OP was used alone, and resistance to the mixture was largely dependent on the presence of the insensitive AChE.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 84 , Issue 2 , June 1994 , pp. 179 - 184
Copyright
Copyright © Cambridge University Press 1994

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