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Aphicidal activity of imidacloprid against a tobacco feeding strain of Myzus persicae (Homoptera: Aphididae) from Japan closely related to Myzus nicotianae and highly resistant to carbamates and organophosphates

Published online by Cambridge University Press:  10 July 2009

Ralf Nauen ,
Jürgen Strobel ,
Klaus Tietjen ,
Yuichi Otsu ,
Christoph Erdelen  and
Alfred Elbert
Ralf Nauen*
Affiliation:
Bayer AG, Agrochemicals Division, Research Insecticides, Institut für Tierische Schädlinge, Leverkusen, Germany
Jürgen Strobel
Affiliation:
University of Stuttgart-Hohenheim, Institute of Zoophysiology, Stuttgart, Germany
Klaus Tietjen
Affiliation:
Bayer AG, Agrochemicals Division, Research Biotechnology, Biochemistry, Leverkusen, Germany
Yuichi Otsu
Affiliation:
Nihon Bayer Agrochem K. K., Yuki Research Center, Ibaraki, Japan
Christoph Erdelen
Affiliation:
Bayer AG, Agrochemicals Division, Research Insecticides, Institut für Tierische Schädlinge, Leverkusen, Germany
Alfred Elbert
Affiliation:
Bayer AG, Agrochemicals Division, Research Insecticides, Institut für Tierische Schädlinge, Leverkusen, Germany
*
Ralf Nauen, Bayer AG, Agrochemicals Division, Research Insecticides, Institut für Tierische Schädlinge, Geb. 6220, D-51368 Leverkusen, Germany.
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Abstract

We investigated the resistance potential of a red-coloured Japanese strain (JR) of a tobacco feeding form of Myzus persicae (Sulzer) of the M. persicae species complex closely related to the tobacco aphid Myzus nicotianae Blackman. Bioassays were performed with a range of insecticides, imidacloprid, nicotine and cartap, thought to act on nicotinic acetylcholine receptors in vivo, as well as with two conventional insecticides, pirimicarb and oxydemeton-methyl, acting on acetylchol-inesterase (AChE). Compared to a susceptible strain, JR showed high resistance to pirimicarb and oxydemeton-methyl, but was far less resistant to nicotine, cartap and imidacloprid. Imidacloprid was, among the insecticides tested, the most active compound in contact and ingestion bioassays. Compared to the susceptible strain, JR showed four-to seven-fold resistance to imidacloprid depending on the type of bioassay. Resistance factors for other insecticides tested in an oral ingestion bioassay were: cartap five-fold, nicotine nine-fold, oxydemeton-methyl 107-fold and pirimicarb > 385-fold. JR showed high carboxylesterase activity. Polyacrylamide gel electrophoresis indicated esterase FE4 as the major carboxylesterase. As for most M. persicae strains and some Greek strains of M. nicotianae, JR was monomorphic for glutamate oxalacetate transaminase. Studies with pirimicarb showed a marked insensitivity of AChE to inhibition by this chemical, whilst such insensitivity could not be detected with the organophosphate insecticide oxydemeton-methyl. Receptor binding assays with [3H]-imidacloprid in aphid homogenates revealed I50-values of 0.4 to 0.8 nM and no statistical difference between the JR and susceptible strain.

Type
Research Article
Information
Bulletin of Entomological Research , Volume 86 , Issue 2 , April 1996 , pp. 165 - 171
Copyright
Copyright © Cambridge University Press 1996

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References

Abdel-Aal, Y.A.I., Lampert, E.P., Roe, R.M. & Semtner, P.J. (1992) Diagnostic esterases and insecticide resistance in the tobacco aphid, Myzus nicotianae Blackman (Homoptera:Aphididae). Pesticide Biochemistry and Physiology 43, 123–133.CrossRefGoogle Scholar
Bai, D., Lummis, S.C.R., Leicht, W., Breer, H & Sattelle, D.B. (1991) Actions of imidacloprid and a related nitromethylene on cholinergic receptors of an identified insect motor neurone. Pesticide Science 33, 197–204.CrossRefGoogle Scholar
Blackman, R.L. (1987) Morphological discrimination of a tobacco-feeding form from Myzus persicae (Sulzer) (Hemiptera:Aphididae) and a key to New World Myzus (Nectarosiphon) species. Bulletin of Entomological Research 77, 713–730.CrossRefGoogle Scholar
Blackman, R.L. & Spence, J.M. (1992) Electrophoretic distinction between the peach–potato aphid, Myzus persicae, and the tobacco aphid, M. nicotianae (Homoptera: Aphididae). Bulletin of Entomological Research 82, 161–165.CrossRefGoogle Scholar
Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–253.CrossRefGoogle ScholarPubMed
Devonshire, A.L. (1989) Insecticide resistance in Myzus persicae: From field to gene and back again. Pesticide Science 26, 375–382.CrossRefGoogle Scholar
Devonshire, A.L. & Moores, G.D. (1982) A carboxylesterase with broad substrate specificity causes organophosphorus, carbamate and pyrethroid resistance in peach–potato aphids(Myzus persicae). Pesticide Biochemistry and Physiology 18, 235–246.CrossRefGoogle Scholar
Devonshire, A.L., Moores, G.D. & Chiang, C.-L. (1983) The biochemistry of insecticide resistance in the peach–potato aphid, Myzus persicae. pp. 191–196in Miyamoto, J. (Eds) IUPAC pesticide chemistry, human welfare and the environment, IUPAC Congress Volume 3, Oxford, Pergamon.Google Scholar
Dewar, A.M., Read, L.A., Hallsworth, P.B. & Smith, H.G. (1992)Effect of imidacloprid on transmission of viruses by aphids in sugar beet. Proceedings of the Brighton Crop Protection Conference – Pests and Diseases 1992, 563–568.Google Scholar
Elbert, A., Becker, B., Hartwig, J. & Erdelen, C. (1991) Imidacloprid –a new systemic insecticide. Pflanzenschutz Nachrichten Bayer 44, 113–136.Google Scholar
Elbert, A., Nauen, R., Cahill, M., Devonshire, A., Sone, S. & Steffens, R. (in press) Resistance management for nicotinyl insecticides using imidacloprid as an example. Pflanzenschutz Nachrichten Bayer 49.Google Scholar
Ellmann, G.L., Courtney, K.D., Anders, V. Jr., & Featherstone, R.M. (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology 7, 88–95.CrossRefGoogle Scholar
FAO (1979) Recommended methods for the detection and measurement of resistance of agricultural pests to pesticides: method for adult aphids – FAO method No. 17. FAO Plant Protection Bulletin 18, 6.Google Scholar
Georghiou, G.P. (1990) Overview of insecticide resistance. pp. 18–41in Green, M.B., LeBaron, H.M. & Moberg, W.K. (Eds) Managing resistance to agrochemicals from fundamental research to practical strategies. Washington DC, American Chemical Society.CrossRefGoogle Scholar
Harlow, C.D., Southern, P.S. & Lampert, E.P. (1991) Geographic distribution, carboxylesterase activity, and chromosome configuration of two color form of the tobacco aphid (Homoptera: Aphididae) in North Carolina. Journal of Economic Entomology 84, 1175–1179.CrossRefGoogle Scholar
Leicht, W. (1993) Imidacloprid – a chloronicotinyl insecticide. Pesticide Outlook 4, 17–24.Google Scholar
Liu, M.-Y. & Casida, J.E. (1993) High affinity binding of [3H]-imidacloprid in the insect acetylcholine receptor. Pesticide Biochemistry and Physiology 46, 40–46.CrossRefGoogle Scholar
Liu, M.-Y., Latli, B. & Casida, J.E. (1994) Nitromethyleneimida-zolidine radioligand ([3H]NMI): high affinity and cooperative binding for house fly acetylcholine receptor. Pesticide Biochemistry and Physiology 50, 171–182.CrossRefGoogle Scholar
Loxdale, H.D., Castanera, P. & Brookes, C.P. (1983) Electrophoretic study of enzymes from cereal aphid populations. I.Electrophoretic techniques and staining systems for characterizing isoenzymes from six species of cereal aphids (Hemiptera:Aphididae). Bulletin of Entomological Research 73, 645–657.CrossRefGoogle Scholar
Mittler, T.E. (1988) Applications of artificial feeding techniques for aphids. pp. 145–171in Minks, A.K. & Harrewijn, P. (Eds) Aphids: their biology natural enemies and control, Volume 2B. Amsterdam, Elsevier.Google Scholar
Moores, G.D., Devine, G.J. & Devonshire, A.L. (1994 a) Insecticide-insensitive acetylcholinesterase can enhance esterase-based resistance in Myzus persicae and Myzus nicotianae. Pesticide Biochemistry and Physiology 49, 114–120.CrossRefGoogle Scholar
Moores, G.D., Devine, G.J. & Devonshire, A.L. (1994 b) Insecticide resistance due to insensitive acetylcholinesterase in Myzus persicae and Myzus nicotianae. Proceedings of the Brighton Crop Protection Conference – Pests and Diseases 1994, 413–418.Google Scholar
Muiiins, J.W. (1993) Imidacloprid: a new nitroguanidine insecticide. pp. 183–197in Duke, S.O., Menn, J.J. & Plimmer, J.R. (Eds) Pest control with enhanced environmental safety, Washington, DC, American Chemical Society.Google Scholar
Nauen, R. (1995) Behaviour modifying effects of low systemic concentrations of imidacloprid on Myzus persicae with special reference to an antifeeding response. Pesticide Science 44, 145–153.CrossRefGoogle Scholar
Nauen, R. & Elbert, A. (1994) Effect of imidacloprid on aphids after seed treatment of cotton in laboratory and greenhouse experiments. Pfanzenschutz Nachrichten Bayer 47, 181–216.Google Scholar
Oppenoorth, F.J. (1985) Biochemistry and genetics of insecticide resistance. pp. 731–773in Kerkut, G.A. & Gilbert, L.I. (Eds) Comprehensive insect physiology, biochemistry and pharmacology, Volume 12. New York, Pergamon.Google Scholar
Silver, A.R.J., Van Emden, H.F. & Battersby, M. (1995) A biochemical mechanism of resistance to pirimicarb in two glasshouse clones of Aphis gossypii. Pesticide Science 43, 21–29.CrossRefGoogle Scholar
Suzuki, K. & Hama, H. (1994) Acetylcholinesterase of the cotton aphid, Aphis gossypii Glover (Homoptera: Aphididae) I. Three clone-types possessing acetylcholinesterases of low and high sensitivity to pirimicarb, and the mixture. Applied Entomology and Zoology 29, 200–303.CrossRefGoogle Scholar
Sylvester, E.S. (1989) Viruses transmitted by aphids. pp. 65–88in Minks, A.K. & Harrewijn, P. (Eds) Aphids: their biology, natural enemies and control, Volume 2C. Amsterdam, Elsevier.Google Scholar
Wellings, P.W., Ward, S.A., Dixon, A.F.G. & Rabbinge, R. (1989) Crop loss assessment. pp. 49–64in Minks, A.K. & Harrewijn, P. (Eds) Aphids: their biology, natural enemies and control. Volume 2C, Amsterdam, Elsevier.Google Scholar
Yamamoto, I., Yabuta, G., Tomizawa, M., Saito, T., Miyamoto, T. & Kagabu, S. (1995) Molecular mechanism for selective toxicity of nicotinoids and neonicotinoids. Journal of Pesticide Science 20, 33–40.CrossRefGoogle Scholar