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The Anopheles maculipennis complex (Diptera: Culicidae) in Iran: molecular characterization and recognition of a new species

Published online by Cambridge University Press:  09 March 2007

M.M. Sedaghat ,
Y.-M. Linton ,
M.A. Oshaghi ,
H. Vatandoost  and
R.E. Harbach
M.M. Sedaghat
Affiliation:
Department of Medical Entomology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Department of Entomology and Biomedical Sciences Theme, The Natural History Museum, Cromwell Road, London, SW7 5BD, UK
Y.-M. Linton
Affiliation:
Department of Entomology and Biomedical Sciences Theme, The Natural History Museum, Cromwell Road, London, SW7 5BD, UK
M.A. Oshaghi
Affiliation:
Department of Medical Entomology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
H. Vatandoost
Affiliation:
Department of Medical Entomology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
R.E. Harbach*
Affiliation:
Department of Entomology and Biomedical Sciences Theme, The Natural History Museum, Cromwell Road, London, SW7 5BD, UK
*
*Fax: (0)20 7942 5229 E-mail: [email protected]
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Abstract

Mosquitoes of the Anopheles maculipennis complex were collected in nine provinces of Iran (Esfahan, Fars, Gilan, Golestan, Kohkiluyeh va Boyerahmad, Mazandaran, Tehran, Azarbaijan-e Gharbi and Zanjan) between June 1983 and September 2002. The nuclear rDNA ITS2 sequences of 86 specimens were compared with those of seven species of the complex available in GenBank. Three genetically distinct species of the complex were distinguished: A. maculipennis Meigen, A. sacharovi Favre and a previously unrecognized species. The last species is most similar to, but clearly distinct from, A. martinius Shingarev and A. sacharovi. The taxonomy of A. martinius and A. sacharovi is critically reviewed, and justification is provided for formally recognizing the third species as Anopheles persiensissp.n. The new species is the first culicid to be characterized and named principally on the basis of DNA evidence. Anopheles persiensis was collected only in the northern Caspian Sea littoral provinces of Gilan and Mazandaran, and it seems likely that this species could be responsible for malaria transmission in this region that was previously attributed to A. maculipennis. A species-specific RFLP–PCR assay based on ITS2 sequences was developed to facilitate further studies of the three species in Iran.

Type
Research Article
Information
Bulletin of Entomological Research , Volume 93 , Issue 6 , December 2003 , pp. 527 - 535
Copyright
Copyright © Cambridge University Press 2003

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