Hostname: page-component-745bb68f8f-v2bm5 Total loading time: 0 Render date: 2025-01-09T23:16:01.717Z Has data issue: false hasContentIssue false
  • English
  • Français

Amplified fragment length polymorphism analysis of different geographic populations of the gypsy moth, Lymantria dispar (Lepidoptera: Lymantriidae)

Published online by Cambridge University Press:  09 March 2007

A. Reineke ,
P. Karlovsky  and
C.P.W. Zebitz
A. Reineke*
Affiliation:
University of Hohenheim, Institute of Phytomedicine, Department of Entomology, D-70593 Stuttgart, Germany
P. Karlovsky
Affiliation:
University of Hohenheim, Institute of Phytomedicine, Department of Entomology, D-70593 Stuttgart, Germany
C.P.W. Zebitz
Affiliation:
University of Hohenheim, Institute of Phytomedicine, Department of Entomology, D-70593 Stuttgart, Germany
*
*Fax: +49 711 4592408 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The gypsy moth, Lymantria dispar Linnaeus, is one of the most serious insect pests of palaearctic and nearctic hardwood forests. We used amplified fragment length polymorphism (AFLP) to detect genetic diversity within and among gypsy moth populations. Five AFLP primer combinations were used on 98 L. dispar samples from different parts of Europe, Asia and North America, detecting a total of 481 polymorphic and 58 monomorphic fragments. Genetic similarities based on these data were calculated and cluster analysis was performed to graphically display groupings between isolates. Lymantria dispar individuals from close geographical areas of Europe were mostly grouped together in cluster analysis resulting in the formation of subgroups corresponding to the origin of the samples. Supporting this observation, clustering of individuals from 22 neighbouring populations in southern Germany agreed well with the region they originated from. Thus, AFLP analysis revealed the existence of a certain degree of genetic variability between European gypsy moth populations that could be explained by the accumulation of polymorphisms resulting from both historical population bottlenecks and the adaptation to different environmental conditions. The results of this study therefore demonstrate that AFLP analysis is a sensitive technique for distinguishing genotypes from different geographic origins as well as from neighbouring local populations and provides sufficient molecular markers for future characterization of the gypsy moth genome.

Type
Review Article
Information
Bulletin of Entomological Research , Volume 89 , Issue 1 , January 1999 , pp. 79 - 88
Copyright
Copyright © Cambridge University Press 1999

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Baranchikov, Y.N. (1989) Ecological basis of the evolution of host relationships in Eurasian gypsy moth populations. pp. 319–338in Wallner, W.E. & McManus, K.A. (Eds) Proceedings, Lymantriidae: a comparison of features of New and Old World tussock mothsUS Department of Agriculture, General Technical Report NE-123.Google Scholar
Bogdanowicz, S.M., Wallner, W.E., Bell, J., O'Dell, T.M. & Harrison, R.G. (1993) Asian gypsy moths (Lepidoptera: Lymantriidae) in North America: evidence from molecular data. Annals of the Entomological Society of America 86, 710–715.CrossRefGoogle Scholar
Bogenschütz, H. (1994) The gypsy moth situation in Germany. pp. 8–9 in Proceedings, US Department of Agriculture interagency gypsy moth research forumAnnapolis, Maryland18–21 January 1994 General Technical Report NE-188.Google Scholar
Cardé, R.T., Charlton, R.E., Wallner, W.E. & Baranchikov, Y.N. (1996) Pheromone-mediated diel activity rhythms of male Asian gypsy moths (Lepidoptera: Lymantriidae) in relation to female eclosion and temperature. Annals of the Entomological Society of America 89, 745–753.CrossRefGoogle Scholar
Chakraborty, R. & Nei, M. (1977) Bottleneck effects on average heterozygosity and genetic distance with the stepwise mutation model. Evolution 31, 347–356.CrossRefGoogle ScholarPubMed
Dice, L.R. (1945) Measures of the amount of ecological association between species. Ecology 26, 297–302.CrossRefGoogle Scholar
Forbush, E.H. & Fernald, C.H. (1896) The gypsy moth. Boston, Massachusetts, Wright and Potter.Google Scholar
Garner, K.J. & Slavicek, J.M. (1996) Identification and characterization of a RAPD-PCR marker for distinguishing Asian and North American gypsy moths. Insect Molecular Biology 5, 81–91.CrossRefGoogle ScholarPubMed
Goldschmidt, R. (1934) Lymantria. Bibliographia Genetica 11, 1–185.Google Scholar
Gossenauer-Marohn, H. (1995) Current status of gypsy moth (Lymantria dispar L.) in Germany. pp. 216–225in Hilburn, D., Johnson, K.R.T. & Mudge, A.D. (Eds) Proceedings, 1994 Annual Gypsy Moth ReviewOregon Department of AgricultureSalem, Oregon.Google Scholar
Harrison, R.G. & ODell, T.M. (1989) Mitochondrial DNA as a tracer of gypsy moths origins. pp. 265–273in Wallner, W.E. & McManus, K.A. (Eds) Proceedings, Lymantriidae: a comparison of features of New and Old World tussock mothsUS Department of Agriculture, General Technical Report NE-123.Google Scholar
Harrison, R.G., Wintermeyer, S.F. and O'Dell, T.M. (1983) Patterns of genetic variation within and among gypsy moth, Lymantria dispar (Lepidoptera: Lymantriidae), populations. Annals of the Entomological Society of America 76, 652–656.CrossRefGoogle Scholar
Janssen, P., Coopman, R., Huys, G., Swings, J., Bleeker, M., Vos, P., Zabeau, M. & Kersters, K. (1996) Evaluation of the DNA fingerprinting method AFLP as a new tool in bacterial taxonomy. Microbiology 142, 1881–1893.CrossRefGoogle ScholarPubMed
Keena, M.A. (1994) Identification of gypsy moth larval color forms. US Department of Agriculture, Forest Service Leaflet NE/NA-INF-123-94.Google Scholar
Keena, M.A. (1996) Comparison of the hatch of Lymantria dispar (Lepidoptera: Lymantriidae) eggs from Russia and the United States after exposure to different temperatures and durations of low temperature. Annals of the Entomological Society of America 89, 564–572.CrossRefGoogle Scholar
Kim, K.C. (1991) Immigrant arthropod pests. Crop Protection 10, 4–5.Google Scholar
Koshio, C. (1996) Pre-ovipositional behaviour of female gypsy moth, Lymantria dispar L. (Lepidoptera, Lymantriidae). Applied Entomology and Zoology 31, 1–10.CrossRefGoogle Scholar
Krider, H.M. & Shields, K.S. (1997) Meiosis in North American and Asian races of gypsy moth (Lepidoptera: Lymantriidae) and their hybrids. Annals of the Entomological Society of America 90, 223–229.CrossRefGoogle Scholar
Leberg, P.L. (1992) Effects of population bottlenecks on genetic diversity as measured by allozyme electrophoresis. Evolution 46, 477–494.CrossRefGoogle ScholarPubMed
Majer, D., Mithen, R., Lewis, B.G., Vos, P. & Oliver, R.P. (1996) The use of AFLP fingerprinting for the detection of genetic variation in fungi. Mycological Research 100, 1107–1111.CrossRefGoogle Scholar
Nagasawa, S. (1988) Number of larval instars of the gypsy moth in Japan (Lepidoptera: Lymantriidae). Applied Entomology and Zoology 23, 441–448.CrossRefGoogle Scholar
Nei, M. & Li, W.H. (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Acadamy of Sciences, USA 76, 5269–5273.CrossRefGoogle ScholarPubMed
Pfeifer, T.A., Humble, L.M., Ring, M. & Grigliatti, T.A. (1995) Characterization of gypsy moth populations and related species using a nuclear DNA marker. Canadian Entomologist 127, 49–58.CrossRefGoogle Scholar
Reineke, A. (1998) Differenzierung von verschiedenen Populationen des Schwammspinners, Lymantria dispar L. (Lepidoptera, Lymantriidae), mit Hilfe molekularer Marker. PhD thesis University of Hohenheim. Stuttgart, Grauer.Google Scholar
Reineke, A. & Zebitz, C.P.W. (1998) Flight ability of gypsy moth females (Lymantria dispar L.) (Lep., Lymantriidae): a behavioural feature characterizing moths from Asia? Journal of Applied Entomology 122, 307–310.CrossRefGoogle Scholar
Reineke, A., Karlovsky, P. & Zebitz, C.P.W. (1998) Preparation and purification of DNA from insects for AFLP-analysis. Insect Molecular Biology 7, 95–99.CrossRefGoogle ScholarPubMed
Schaefer, P.W., Weseloh, R.M., Sun, X., Wallner, W.E. & Yan, J. (1984) Gypsy moth, Lymantria (=Ocneria) dispar (L.) (Lepidoptera: Lymantriidae), in the People's Republic of China. Environmental Entomology 13, 1535–1541.CrossRefGoogle Scholar
Schedl, K.E. (1936) Der Schwammspinner in Eurasien, Afrika und Neuengland. Monographien zur Angewandten Entomologie 12. Berlin, Parey.Google Scholar
Schreiber, D.E., Garner, K.J. & Slavicek, J.M. (1997) Identification of three randomly amplified polymorphic DNA-polymerase chain reaction markers for distinguishing Asian and North American gypsy moths (Lepidoptera: Lymantriidae). Annals of the Entomological Society of America 90, 667–674.CrossRefGoogle Scholar
Sharma, S.K., Knox, M.R. & Ellis, T.H.N. (1996) AFLP analysis of the diversity and phylogeny of Lens and its comparison with RAPD analysis. Theoretical and Applied Genetics 93, 751–758.CrossRefGoogle ScholarPubMed
Sneath, P.H.A. & Sokal, R.R. (1973) Numerical taxonomy. San Francisco, California, Freeman and Company.Google Scholar
Spuler, A. (1908) Die Schmetterlinge Europas. Vol 1. Stuttgart, E.SchweizerbartscheVerlagsbuchhandlung.Google Scholar
Taylor, R.A.J. & Reling, D. (1986) Density/height profile and long-range dispersal of first-instar gypsy moth (Lepidoptera: Lymantriidae). Environmental Entomology 15, 431–435.CrossRefGoogle Scholar
Vos, P., Hogers, R., Bleeker, M., Reijans, M., Van De Lee, T., Hornes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M. & Zabeau, M. (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research 23, 4407–4414.CrossRefGoogle ScholarPubMed
Wallner, W.E., Grinberg, P.S. & Walton, G.S. (1994) Differentiation between gypsy moth (Lepidoptera: Lymantriidae) populations by spectral color discrimination of head capsules. Environmental Entomology 23, 659–664.CrossRefGoogle Scholar
Wallner, W.E., Humble, L.M., Levin, R.E., Baranchikov, Y.N. and Cardé, R.T. (1995) Response of adult lymantriid moths to illumination devices in the Russian Far East. Journal of Economic Entomology 88, 337–342.CrossRefGoogle Scholar