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Age-dependent changes in the ratio of (R)- and (S)-2-butanol released by virgin females of Dasylepida ishigakiensis (Coleoptera: Scarabaeidae)

Published online by Cambridge University Press:  10 July 2012

N. Fujiwara-Tsujii ,
H. Yasui ,
S. Wakamura ,
F. Mochizuki  and
N. Arakaki
N. Fujiwara-Tsujii*
Affiliation:
Laboratory of Insect Interaction Research, National Institute of Agrobiological Sciences (NIAS), Ohwashi, Tsukuba, Ibaraki 305-0851, Japan
H. Yasui
Affiliation:
Laboratory of Insect Interaction Research, National Institute of Agrobiological Sciences (NIAS), Ohwashi, Tsukuba, Ibaraki 305-0851, Japan
S. Wakamura
Affiliation:
Laboratory of Insect Interaction Research, National Institute of Agrobiological Sciences (NIAS), Ohwashi, Tsukuba, Ibaraki 305-0851, Japan
F. Mochizuki
Affiliation:
Specialty Chemicals Research Center, Shin-Etsu Chemical Co., Ltd, Kubiki, Joetsu, Niigata 942-8601, Japan
N. Arakaki
Affiliation:
Okinawa Prefectural Agricultural Research Center (OPARC), Itoman, Okinawa 901-0336, Japan
*
*Author for correspondence Fax:+81-(0)29-838-6205 E-mail: [email protected]
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Abstract

The females of the white grub beetle, Dasylepida ishigakiensis, release two enantiomers of 2-butanol, (R)-2-butanol and (S)-2-butanol. The ratio describing the relative proportions of these two enantiomers (R/S ratio) has not yet been investigated. (R)-2-Butanol has been shown to attract males in laboratory and field experiments, whereas (S)-2-butanol tends to inhibit them. To determine the R/S ratio of the 2-butanol emitted by virgin females, we collected 2-butanol from young (53 days old), mature (63 days old) and old females (73 days old) using water, extracted with an SPME fibre and subsequently injected into GC-MS. The major component of the 2-butanol emitted by the young females was (R)-2-butanol, but as the females aged, the component ratio favoured (S)-2-butanol. Young females released an 80:20 mixture of (R)- and (S)-2-butanol, whereas old females released a 45:55 mixture. The EAG response of male antennae to a 50:50 ratio (racemic mixture) showed a similar dose-response curve to that of (R)-2-butanol. The male orientation responses to (R)-2-butanol decreased when the relative proportion of (S)-2-butanol increased. An inhibitory and/or masking effect of (S)-2-butanol on male orientation behaviour was also observed in the flight tunnel assay. These results suggest that males are more strongly attracted to young females than to old females. We also discuss the possibility of using 2-butanol isomers as a control or monitoring agent for this insect.

Keywords

age-dependent2-butanolDasylepida ishigakiensisR/S ratiosex attractant pheromone
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 102 , Issue 6 , December 2012 , pp. 730 - 736
Copyright
Copyright © Cambridge University Press 2012

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