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Testing mate recognition through reciprocal crosses of two native populations of the whitefly Bemisia tabaci (Gennadius) in Australia

Published online by Cambridge University Press:  23 October 2019

W. Wongnikong Open the ORCID record for W. Wongnikong [Opens in a new window] ,
S. L. van Brunschot Open the ORCID record for S. L. van Brunschot [Opens in a new window] ,
J. P. Hereward Open the ORCID record for J. P. Hereward [Opens in a new window] ,
P. J. De Barro  and
G. H. Walter Open the ORCID record for G. H. Walter [Opens in a new window]
W. Wongnikong*
Affiliation:
School of Biological Sciences, The University of Queensland, Brisbane, Queensland4072, Australia
S. L. van Brunschot
Affiliation:
School of Biological Sciences, The University of Queensland, Brisbane, Queensland4072, Australia Agriculture, Health & Environment Department, Natural Resources Institute, University of Greenwich, Medway Campus, Central Avenue, Chatham Maritime ME4 4TB, UK
J. P. Hereward
Affiliation:
School of Biological Sciences, The University of Queensland, Brisbane, Queensland4072, Australia
P. J. De Barro
Affiliation:
CSIRO Health & Biosecurity, GPO Box 2583, Brisbane QLD 4001, Australia
G. H. Walter
Affiliation:
School of Biological Sciences, The University of Queensland, Brisbane, Queensland4072, Australia
*
Author for correspondence: W. Wongnikong, E-mail: [email protected]
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Abstract

Bemisia tabaci (Gennadius) represents a relatively large cryptic species complex. Australia has at least two native populations of B. tabaci sensu lato and these were first found on different host plants in different parts of Australia. The species status of these populations has not been resolved, although their mitochondrial sequences differ by 3.82–4.20%. We addressed the question of whether these AUSI and AUSII B. tabaci populations are distinct species. We used reciprocal cross-mating tests to establish whether the insects from these different populations recognize one another as potential mating partners. The results show that the two native Australian populations of B. tabaci have a mating sequence with four phases, each of which is described. Not all pairs in the control crosses mated and the frequency of mating differed across them. Some pairs in the AUSI-M × AUSII-F did mate (15%) and did produce female progeny, but the frequency was extremely low relative to controls. Microsatellite genotyping of the female progeny produced in the crosses showed these matings were successful. None of the AUSII-M × AUSI-F crosses mated although some of the males did search for females. These results demonstrate the critical role of the mate recognition process and the need to assess this directly in cross-mating tests if the species status of different populations is to be tested realistically. In short, AUSI and AUSII B. tabaci populations are distinct species because the individual males and females do not recognize individuals of the alternative population as potential mating partners.

Keywords

Bemisia tabacimate recognitionmicrosatellite genotypingreciprocal crossesspecies concepts
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 110 , Issue 3 , June 2020 , pp. 328 - 339
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Copyright
Copyright © Cambridge University Press 2019

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