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A PCR-based approach to distinguish important Diadegma species (Hymenoptera: Ichneumonidae) associated with diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae)

Published online by Cambridge University Press:  09 March 2007

B. Wagener ,
A. Reineke ,
B. Löhr  and
C.P.W. Zebitz
B. Wagener*
Affiliation:
International Centre of Insect Physiology and Ecology, PO Box 30772, 00100 GPO Nairobi, Kenya University of Hohenheim, Institute of Phytomedicine, Department of Entomology, D-70593 Stuttgart, Germany
A. Reineke
Affiliation:
University of Hohenheim, Institute of Phytomedicine, Department of Entomology, D-70593 Stuttgart, Germany
B. Löhr
Affiliation:
International Centre of Insect Physiology and Ecology, PO Box 30772, 00100 GPO Nairobi, Kenya
C.P.W. Zebitz
Affiliation:
University of Hohenheim, Institute of Phytomedicine, Department of Entomology, D-70593 Stuttgart, Germany
*
*Fax: +254 20 860110 Email: [email protected]
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Abstract

The diamondback moth, Plutella xylostella (Linnaeus) has a cosmopolitan distribution and is one of the major pests on cruciferous plants. Biological control, especially with species of the genus Diadegma, has been successfully employed in several parts of the world, mainly in South East Asia. The taxonomy of this genus based on classical morphological characters is still unclear and misidentifications are reported. In the present study seven Diadegma species associated with P. xylostella were separated using polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) analyses. The second internal transcribed spacer (ITS2) of the ribosomal DNA (rDNA) was successfully amplified in all 167 individuals and digested using 11 different restriction enzymes. One restriction enzyme (CfoI) showed different restriction profiles in all species and also between two population samples of D. mollipla (Holmgren) from eastern and southern Africa. In addition, a new Diadegma species associated with P. xylostella from Ethiopia was discovered.

Type
Review Article
Information
Bulletin of Entomological Research , Volume 94 , Issue 5 , October 2004 , pp. 465 - 471
Copyright
Copyright © Cambridge University Press 2004

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References

Azidah, A.A., Fitton, M.G. & Quicke, D.L.J. (2000) Identification of Diadegma species (Hymenoptera: Ichneumonidae, Campopleginae) attacking the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae). Bulletin of Entomological Research 90, 375–389.CrossRefGoogle ScholarPubMed
Baruffi, L., Damiani, G., Guglielmino, C.R., Bandi, C., Malacrida, A.R. & Gasperi, G. (1995) Polymorphism within and between populations of Ceratitis capitata: comparison between RAPD and multilocus enzyme electrophoresis data. Heredity 74, 425–437.CrossRefGoogle ScholarPubMed
Brown, L., Olfert, O., Soroka, J., Mason, P. & Dosdall, L. (2002) Diamondback moth biocontrol activities in Canada. pp. 144–146 in Kirk, A.A. & Bordat, D. (Eds) Improving biocontrol of Plutella xylostella. Proceedings of the international symposium21–24 October 2002Montpellier, France.Google Scholar
Caterino, M.S., Cho, C. & Sperling, F.A.H. (2000) The current state of insect molecular systematics: a thriving tower of Babel. Annual Review of Entomology 45, 1–54.CrossRefGoogle ScholarPubMed
Chu, Y.-I. (1986) The migration of diamondback moth. (Eds) Diamondback moth management: pp. 77–81 in Talekar, N.S., Griggs, T.D. 11–15 March, 1985, proceedings of the first international workshop. Tainan, Taiwan. Shanhua Asian Vegetable Research and Development CenterGoogle Scholar
Fitton, M. & Walker, A. (1992) Hymenopterous parasitoids associated with diamondback moth: a taxonomic dilemma. pp. 225–232. Talekar, N.S. (Eds). 10–14 December, 1990, Diamondback moth and other crucifer pests: proceedings of the second international workshop. Tainan, Taiwan. Taipei Asian Vegetable Research and Development CenterGoogle Scholar
Gallego, D. & Galian, J. (2001) The internal transcribed spacers (ITS1 and ITS2) of the rDNA differentiate the bark beetle forest pests Tomicus destruens and T. piniperda. Insect Molecular Biology 10, 415–420.CrossRefGoogle ScholarPubMed
Hackett, B.J., Gimnig, J., Guelbeogo, W., Costantini, C., Koekemoer, L.L., Coetzee, M. & Collins, F.H. (2000) Ribosomal DNA internal transcribed spacer (ITS2) sequences differentiate Anopheles funestus and An. rivulorum, and uncover a cryptic taxon. Insect Molecular Biology 9, 369–374.CrossRefGoogle Scholar
Hardy, J.E. (1938) Plutella maculipennis Curt., its natural and biological control in England. Bulletin of Entomological Research 29, 343–372.CrossRefGoogle Scholar
Hillis, D.M. & Dixon, M.T. (1991) Ribosomal DNA: molecular evolution and phylogenetic inference. Quarterly Review of Biology 66, 411–447.CrossRefGoogle ScholarPubMed
Johnson, M.W., Mitchell, W.C., Robin, M.R., Cushing, N.L. & Rethwisch, M.D. (1988) Parasitization of the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), in Hawaii. Proceedings of the Entomological Society of Hawaii 28, 197–203.Google Scholar
Kfir, R. (1998) Origin of the diamondback moth (Lepidoptera: Plutellidae). Annals of the Entomological Society of America 91, 164–167.CrossRefGoogle Scholar
Knapp, M., Wagener, B. & Navajas, M. (2003) Molecular discrimination between the spider mite Tetranychus evansi Baker & Pritchard, an important pest of tomatoes in southern Africa, and the closely related species T. urticae Koch (Acarina: Tetranychidae). African Entomology 11, 300–304.Google Scholar
Lim, G.-S. (1986) Biological control of diamondback moth. pp. 159–171 in Talekar, N.S., Griggs, T.D.Diamondback moth management: proceedings of the first international workshop11–15 March, 1985Tainan, Taiwan.Shanhua, Asian Vegetable Research and Development Center.Google Scholar
Liu, S.-S., Wang, X.-G., Guo, S.-J., He, J.-H., Shi, Z.-J. (2000) Seasonal abundance of the parasitoid complex associated with diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae) in Hangzhou, China. Bulletin of Entomological Research 90, 221–231.CrossRefGoogle ScholarPubMed
Mustata, G. (1992) Role of parasitoid complex in limiting the population of diamondback moth in Moldavia, Romania. pp. 203–211 in Talekar, N.S.Diamondback moth and other crucifer pests: proceedings of the second international workshop. 10–14 December, 1990, Tainan, Taiwan. Taipei Asian Vegetable Research and Development CenterGoogle Scholar
Navajas, M., Lagnel, J., Gutierrez, J. & Boursot, P. (1998) Species-wide homogeneity of nuclear ITS2 sequences in the spider mite Tetranychus urticae contrasts with extensive mitochondrial COI polymorphism. Heredity 80, 742–752.CrossRefGoogle ScholarPubMed
Prinsloo, G., Chen, Y., Giles, K.L. & Greenstone, M.H. (2002) Release and recovery in South Africa of the exotic aphid parasitoid Aphelinus hordei verified by the polymerase chain reaction. BioControl 47, 127–136.CrossRefGoogle Scholar
Saiki, R.K., Gelfand, D.H., Stoffel, S., Scharf, S.J., Higuchi, R., Horn, G.T., Mullis, K.B. & Erlich, H.A. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239, 487–491.CrossRefGoogle ScholarPubMed
Syed, A.R. (1992) Insecticide resistance in the diamondback moth in Malaysia. pp. 437–442 in Talekar, N.S. (Eds) Diamondback moth and other crucifer pests: proceedings of the second international workshop. 10–14 December, 1990, Tainan, Taiwan. Taipei Asian Vegetable Research and Development Center.Google Scholar
Talekar, N.S. & Shelton, A.M. (1993) Biology, ecology, and management of the diamondback moth. Annual Review of Entomology 38, 275–301.CrossRefGoogle Scholar
Toda, S. & Komazaki, S. (2002) Identification of thrips species (Thysanoptera: Thripidae) on Japanese fruit trees by polymerase chain reaction and restriction fragment length polymorphism of the ribosomal ITS2 region. Bulletin of Entomological Research 92, 359–363.CrossRefGoogle ScholarPubMed
Verkerk, R.H.J. & Wright, D.J. (1996) Multitrophic interactions and management of diamondback moth: a review. Bulletin of Entomological Research 86, 205–216.CrossRefGoogle Scholar