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PCR based monitoring of specific Drosophila (Diptera: Drosophilidae) cyclodiene resistance alleles in the presence and absence of selection

Published online by Cambridge University Press:  10 July 2009

K. Aronstein
Affiliation:
Department of Entomology, University of Wisconsin-Madison, USA
P. Ode
Affiliation:
Department of Entomology, University of Wisconsin-Madison, USA
R.H. ffrench-Constant*
Affiliation:
Department of Entomology, University of Wisconsin-Madison, USA
*
Dr R.H. ffrench-Constant, Department of Entomology, 237 Russell Laboratories, 1630 Linden Drive, University of Wisconsin-Madison, Madison, WI 53706, USA.

Abstract

Cyclodiene insecticide resistance persists in field populations of Drosophila spp. at a frequency of approximately 1% (0.01), despite the withdrawal of most cyclodiene type insecticides except endosulfan. However, we have previously documented that resistance-associated amino acid replacements in the gene Rdl, a γ-aminobutyric acid receptor, can significantly affect several channel functions of the integral chloride ionophore. We were therefore interested in investigating if different resistance-associated replacements confer significant fitness disadvantages and whether the use of endosulfan could be maintaining selection for cyclodiene resistance in the field. Using PCR amplification of specific alleles (PASA) within 3000 individual flies, we report that neither the alanine302 > serine (allele 1) replacement in Drosophila melanogaster Meigen nor the alanine302 > serine (allele 1) or alanine302 > glycine (allele 2) replacements in D. simulans Sturtevant showed any reduction in frequency in cage experiments run for one year in the laboratory in the absence of selection. Further, repeated applications of endosulfan selected significantly for cyclodiene resistance in the field. Thus the apparent absence of fitness cost, combined with the continued use of endosulfan, may maintain cyclodiene resistance at this relatively high frequency in field populations.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1995

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References

Aronstein, K., Ode, P. & ffrench-Constant, R.H. (1994) Direct comparison of PCR based monitoring for cyclodiene resistance in Drosophila populations with insecticide bioassay. Pesticide Biochemistry and Physiology 48, 229233.CrossRefGoogle Scholar
Croft, B.A. & Dunley, J. (1993) Habitat patterns and pesticide resistance. pp. 145162in Kim, K.C. & McPheron, B.A. (Eds), Evolution of insect pests. New York, Wiley and Sons.Google Scholar
ffrench-Constant, R.H. & Devonshire, A.L. (1986) The effect of aphid immigration on the rate of selection of insecticide resistance in Myzus persicae by different classes of insecticides. pp. 115125in Aspects of Applied Biology, 13. Crop protection of sugar beet and erop protection and quality of potatoes. Wellesbourne, AAB.Google Scholar
ffrench-Constant, R.H. & Devonshire, A.L. (1987) A multiple homogenizer for rapid sample preparation in immunoassays and electrophoresis. Biochemical Genetics 25, 493499.CrossRefGoogle ScholarPubMed
ffrench-Constant, R.H., Devonshire, A.L. & Clark, S.J. (1987) Differential rate of selection for resistance by carbamate, organophosphorus and combined pyrethroid and organophosphorus insecticides in Myzus persicae (Sulzer) (Hemiptera: Aphididae). Bulletin of Entomological Research 77, 227238.CrossRefGoogle Scholar
ffrench-Constant, R.H., Devonshire, A.L. & White, R.P. (1988) Spontaneous loss and reselection of resistance in extremely resistant Myzus persicae (Sulzer). Pesticide Biochemistry and Physiology 30, 110.CrossRefGoogle Scholar
ffrench-Constant, R.H., Rocheleau, T.A., Steichen, J.C. & Chalmers, A.E. (1993a) A point mutation in a Drosophila GABA receptor confers insecticide resistance. Nature 363, 449451.CrossRefGoogle Scholar
ffrench-Constant, R.H., Roush, R.T., Mortlock, D. & Dively, G.P. (1990) Isolation of dieldrin resistance from field populations of Drosophila melanogaster (Diptera: Drosophilidae). Journal of Economic Entomology 83, 17331737.CrossRefGoogle Scholar
ffrench-Constant, R.H., Steichen, J., Rocheleau, T.A., Aronstein, K. & Roush, R.T. (1993b) A single-amino acid substitution in a γ-aminobutyric acid subtype A receptor locus associated with cyclodiene insecticide resistance in Drosophila populations. Proceedings of the National Academy of Sciences 90, 19571961.CrossRefGoogle Scholar
ffrench-Constant, R.H., Steichen, J.C. & Ode, P. (1993c) Cyclodiene insecticide resistance in Drosophila melanogaster (Meigen) is associated with a temperature sensitive phenotype. Pesticide Biochemistry and Physiology 46, 7377.CrossRefGoogle Scholar
Georghiou, G.P. (1972) The evolution of resistance to pesticides. Annual Review of Ecology and Systematics 3, 133168.CrossRefGoogle Scholar
Georghiou, G.P. & Taylor, C.E. (1977) Genetic and biological influences in the evolution of insecticide resistance. Journal of Economic Entomology 70, 319323.CrossRefGoogle ScholarPubMed
McKenzie, J.A. (1990) Selection at the dieldrin resistance locus in overwintering populations of Lucilia cuprina (Widemann). Australian Journal of Zoology 38, 493501.CrossRefGoogle Scholar
Otsuka, M., Iversen, L.L., Hall, Z.W. & Kravitz, E.A. (1966) Release of gamma-aminobutyric acid from inhibitory nerves of lobster. Proceedings of the National Academy of Sciences U.S.A. 56, 11101115.CrossRefGoogle ScholarPubMed
Roush, R.T. & McKenzie, J.A. (1987) Ecological genetics of insecticide resistance. Annual Review of Entomology 32, 361380.CrossRefGoogle Scholar
Roush, R.T. & Plapp, F.W. (1982) Effects of insecticide resistance on biotic potential of the house fly (Diptera: Muscidae). Journal of Economic Entomology 75, 708713.CrossRefGoogle ScholarPubMed
Rowland, M. (1988) Management of γ-HCH/dieldrin resistance in mosquitoes-a strategy for all insects? pp. 495500in Proceedings of the British Insecticides and Fungicides Conference 1988, Brighton, British Crop Protection Council.Google Scholar
Steichen, J.C. & ffrench-Constant, R.H. (1994) Amplification of specific cyclodiene insecticide resistance alleles by the polymerase chain reaction. Pesticide Biochemistry and Physiology 48, 17.CrossRefGoogle Scholar
Thompson, M., Steichen, J.C. & ffrench-Constant, R.H. (1993) Conservation of cyclodiene insecticide resistance associated mutations in insects. Insect Molecular Biology 2, 149154.CrossRefGoogle ScholarPubMed
Usherwood, P.N.R. & Grundfest, H. (1965) Peripheral inhibition in skeletal muscle of insects. Journal of Neurophysiology 28, 497518.CrossRefGoogle ScholarPubMed
Whitehead, J.R., Roush, R.T. & Norment, B.R. (1985) Resistance stability and coadaption in diazinon-resistant house flies (Diptera: Muscidae). Journal of Economic Entomology 78, 2529.CrossRefGoogle Scholar
Zhang, H.-G., ffrench-Constant, R.H. & Jackson, M.B. (1994) A unique amino acid of the Drosophila GABA receptor with influence on drug sensitivity by two mechanisms. Journal of Physiology 479, 6575.CrossRefGoogle ScholarPubMed