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A novel set of microsatellite markers for the European Grapevine Moth Lobesia botrana isolated using next-generation sequencing and their utility for genetic characterization of populations from Europe and the Middle East

Published online by Cambridge University Press:  08 April 2015

A. Reineke ,
H.A. Assaf ,
D. Kulanek ,
N. Mori ,
A. Pozzebon  and
C. Duso
A. Reineke*
Affiliation:
Department of Phytomedicine, Geisenheim University, D-65366 Geisenheim, Germany
H.A. Assaf
Affiliation:
Department of Phytomedicine, Geisenheim University, D-65366 Geisenheim, Germany Department of Agronomy, Food, Natural Resources, Animals and the Environment, University of Padova, 35020 Legnaro (Padova), Italy
D. Kulanek
Affiliation:
Department of Phytomedicine, Geisenheim University, D-65366 Geisenheim, Germany
N. Mori
Affiliation:
Department of Agronomy, Food, Natural Resources, Animals and the Environment, University of Padova, 35020 Legnaro (Padova), Italy
A. Pozzebon
Affiliation:
Department of Agronomy, Food, Natural Resources, Animals and the Environment, University of Padova, 35020 Legnaro (Padova), Italy
C. Duso
Affiliation:
Department of Agronomy, Food, Natural Resources, Animals and the Environment, University of Padova, 35020 Legnaro (Padova), Italy
*
*Author for correspondence E-mail: [email protected]
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Abstract

Using a high-throughput 454 pyrosequencing approach a novel set of microsatellite markers was developed for one of the key grapevine insect pests, the European grapevine moth Lobesia botrana (Lepidoptera: Tortricidae). 20 primer pairs flanking a microsatellite motif were designed based on the sequences obtained and were subsequently evaluated in a sample of 14 L. botrana populations from Europe and the Middle East. 11 markers showed stable and reproducible amplification patterns; however, one of the 11 markers was monomorphic in all L. botrana populations analysed. Estimated frequencies of null alleles of more than 20% were evident for two of the markers tested, but varied substantially depending on the respective L. botrana population. In 12 of the 14 L. botrana populations observed heterozygosities were lower to those expected under Hardy–Weinberg equilibrium, indicating a deficiency of heterozygotes in the respective populations. The overall FST value of 0.075 suggested a moderate but significant genetic differentiation between the L. botrana populations included in this study. In addition, a clear geographic structure was detected in the set of samples, evident through a significant isolation by distance and through results from structure analysis. In structure analysis, L. botrana populations were grouped in two clearly separated clusters according to their European (Spain, Italy, Germany) or Middle Eastern (Israel, Syria, Turkey) origin. This novel set of microsatellite markers can now be applied to study the evolutionary ecology of this species including host shifts and host adaptation as well as spread of individuals across worldwide viticulture.

Keywords

microsatellitesSSR markersLobesia botrana454 pyrosequencingpopulation genetics
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 105 , Issue 4 , August 2015 , pp. 408 - 416
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Copyright
Copyright © Cambridge University Press 2015 

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